Purified anti-MMP8 Antibody

Pricing & Availability
Clone
1502022 (See other available formats)
Other Names
Matrix metallopeptidase 8, CLG1, MMP8, PMN-CL, Neutrophil Collagenase, MMP-8
Isotype
Mouse IgG2b, κ
Ave. Rating
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Product Citations
publications
1_1502022_PURE_MMP8_Antibody_1_WB_062816
  • 1_1502022_PURE_MMP8_Antibody_1_WB_062816
  • 2_1502022_PURE_MMP8_Antibody_2_WB_062816
    Western blot analysis of 50 ng recombinant human MMP8 untreated (lane 1), 50 ng MMP8 activated with 1mM p-aminophenylmercuric acetate (APMA) for 45 minutes (lane 2), 15 µg human Neutrophil (lane 3) and 15 µg mouse bone marrow (lane 4). Purified anti-MMP8 antibody (clone 1502022) was used, followed by HRP anti-mouse IgG antibody.
  • 3_1502022_PURE_MMP8_Antibody_IHC_010518
    IHC staining of purified anti‐MMP8 antibody (clone 1502022) on formalin‐fixed paraffin‐embedded normal human spleen. After antigen retrieval using sodium citrate, the tissue was incubated with the primary antibody at 0.5 µg/ml for 24 hours at 4°C. BioLegend’s Ultra Streptavidin (USA) HRP Detection Kit was used for detection followed by hematoxylin counterstaining.
Cat # Size Price Quantity Avail. Save
688002 100 µg 312€
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Description

MMP-8, also termed neutrophil collagenase, is a member of the matrix metalloproteinase family proteins (MMPs). Members of this family are zinc-containing enzymes that degrade the extracellular matrix (ECM) and connective tissue proteins. Substrates of MMP-8 include type I, II, and III triple-helical collagens, gelatin peptides, proteoglycans, fibronectin, aggrecan, substance P, serpins, β-casein, and angiotensin. MMP-8 is released by neutrophils upon IL-1β, IL-8, GM-CSF and TNF-α stimulation. Besides playing a role in phagocytosis, MMP-8 has been implicated in rheumatoid arthritis (RA) because of its high efficiency in infiltrating connective tissue and breaking down ECM. In fact, serum level of MMP-8 is a strong predictor of mortality in RA, as this is consistent with increased activation of neutrophils in RA. In addition, MMP-8 has been shown to play a crucial role in cardiovascular diseases by promoting atherosclerotic lesion formation. This is supported by the finding that MMP-8 knockout mice have less endothelial cells or plaque angiogenesis in the atherosclerotic plaques. In a clinical study, it was shown that the ratio of MMP-9/TIMP-1, as well as MMP-8 serum levels, could be a useful predictor of prognosis in patients with hepatocellular carcinomas.

Product Details
Technical Data Sheet (pdf)

Product Details

Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Human MMP-8, amino acids (Phe21-Gly467) (Accession# NP_002415.1), is expressed with a C-terminal His tag and a linker sequence in 293E cell lines.
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

WB - Quality tested
IHC-P - Validated

Recommended Usage

Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 0.25 - 1.0 µg per ml. For immunohistochemical staining on formalin-fixed paraffin-embedded tissue sections, the suggested use of this reagent is 0.5 - 5.0 µg per ml. It is recommended that the reagent be titrated for optimal performance for each application.

RRID
AB_2617008 (BioLegend Cat. No. 688002)

Antigen Details

Structure
467 amino acids, SDS-PAGE apparent molecular weight of Pro-MMP8 is 65-75 kD and Mature MMP8 is 55-60 kD. Consists of a prodomain that is cleaved upon activation, a catalytic domain, a hinge region and a carboxyl terminal domain with hemopexin-like repeats
Distribution

Neutrophils, macrophages, and monocytes.

Interaction
Collagen type I, II, and III, gelatin, proteoglycans, fibronectin, aggrecan, substance P, serpins, ß-casein, and angiotensin.
Cell Type
Neutrophils, Macrophages, Monocytes
Biology Area
Angiogenesis, Cell Adhesion, Cell Biology, Neuroinflammation, Neuroscience
Molecular Family
Enzymes and Regulators
Antigen References

1. Nagase H, Woessner JF Jr. 1999. J. Biol. Chem. 274:21491.
2. Gill SE, Parks WC. 2008. Int. J. Biochem. Cell Biol. 40:1334.
3. Mattey DL, et al. 2012. Arthritis Res. Ther. 14:R204.
4. Fang C, et al. 2013. Cardiovasc. Res. 99:146.
5. Lempinen M, et al. 2013. Ann. Med. 45:482.
6. Sommer K, et al. 2013. PLoS One 8:e73992.

Gene ID
4317 View all products for this Gene ID
UniProt
View information about MMP-8 on UniProt.org

Related FAQs

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Go To Top Version: 3    Revision Date: 07/01/2016

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