- M4110F03 (See other available formats)
- Regulatory Status
- Other Names
- CLG, CLGN, Interstitial collagenase, Fibroblast collagenase, MMP1
- Mouse IgG1, κ
- Ave. Rating
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- Product Citations
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Members of the matrix metalloproteinase family proteins (MMPs) are structurally related and zinc-containing enzymes that not only degrade the extracellular matrix (ECM) and connective tissue proteins under normal physiological setting but also during disease (e.g. arthritis and metastasis). The MMP family includes six different groups of enzymes: collagenases, gelatinases, stromelysins, transmembrane MMPs, matrilysins and others. MMPs are secreted as proenzymes that have to be cleaved in order to be activated. MMP1 consists of a pro-domain which is cleaved upon activation, a catalytic domain containing the zinc binding site, a short hinge region, and a carboxyl terminal (hemopexin-like repeats) domain. It is thought to play an important role in rheumatoid and osteo-arthritis pathogenesis as mediators of cartilage and bone destruction.
Substrates of MMP-1 include collagen I, II, III, VII, VIII, and X as well as casein, gelatin, alpha1 antitrypsin, myelin basic protein, L Selectin, pro-TNF, IL-1β, IGFBP3, IGFBP5, pro-MMP-2, and pro-MMP-9. In addition, chemokines are substrates for MMP-1. CCL2, CCL7, CCL8, and CCL13 are catalytically cleaved by MMP-1 to produce receptor antagonists. CXCL5 and CXCL12 are also substrates for MMP1. TIMPs inhibit MMPs in a 1:1 inhibitor to enzyme ratio through interaction of the N-terminal domain of the TIMP molecule with the active site of the MMP. MMP1 is overexpressed in invasive melanoma, colorectal, and esophageal cancers. Importantly, MMP-1 expression was shown to increase progressively with tumor stage.
- Verified Reactivity
- Antibody Type
- Host Species
- Recombinant human MMP-1.
- This antibody is provided in phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
- Affinity purified.
- 0.5 mg/ml
- Storage & Handling
- Upon receipt, store undiluted between 2°C and 8°C.
WB - Quality tested
- Recommended Usage
Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 1.0 - 1.25 µg per ml. It is recommended that the reagent be titrated for optimal performance for each application.
AB_2565606 (BioLegend Cat. No. 675702)
- Monomer, belongs to the peptidase M10A family. 469 amino acids, predicted molecular weight of 54 kD.
- Extracellular. MMP-1 is expressed by fibroblasts, keratinocytes, endothelial cells, monocytes, and macrophages.
- MMP1 cleaves collagen of types I, II, III, VII and X. MMP-1 is thought to play an important role in the pathogenesis of rheumatoid and osteo-arthritis as mediators of cartilage and bone destruction. It is also involved in angiogenesis, tissue remodeling and cancer metastasis. MMP-1 regulates chemokine activity. MMP1 is inhibited by TIMPs, Î±2-macroglobulin (inhibitor of MMPs in bodily fluids). TNF-alpha; down-regulates TIMPs and enhances the expression of MMPs.
- MCP1, MCP3, MCP4, TIMP-1, MMP7, Collagen type II α1, IBP-3, Integrin α2, CD44, CD147, Brevican, Chymase 1, Aggrecan 1.
- Biology Area
- Angiogenesis, Cell Adhesion, Cell Biology, Neuroinflammation, Neuroscience
- Molecular Family
- Enzymes and Regulators
- Antigen References
1. Nagase H and Woessner JF Jr. 1999. J. Biol. Chem. 274:21491.
2. Boire A, et al. 2005. Cell 120:303.
3. Kader AK, et al. 2007. Clin. Cancer Res. 13:2614.
4. Gill SE and Parks WC. 2008. Int. J. Biochem. Cell Biol. 40:1334.
5. Mazor R, et al. 2013. J. Biol. Chem. 288:598.
6. Sommer K, et al. 2013. PLos One 8:e73992.
- Gene ID
- 1029 View all products for this Gene ID
- View information about MMP-1 on UniProt.org
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