Purified anti-MEK1 Phospho (Ser298) Antibody

Pricing & Availability
Clone
A16117B (See other available formats)
Regulatory Status
RUO
Other Names
MEK1, MAP2K1, CFC3, MKK1, MAPKK1, PRKMK1
Isotype
Mouse IgG1, κ
Ave. Rating
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Product Citations
publications
A16117B_Purified_MEK1_Phospho_Antibody_050719.png
Whole cell extracts (15 µg protein) from HeLa cells untreated (-) or treated (+) with 300 ng/mL nocodazole overnight were resolved on a 4-12% Bis-Tris gel, transferred to nitrocellulose and probed with 0.0625 µg/mL (1:8000 dilution) of purified anti-MEK1 Phospho (Ser298) Antibody, clone A16117B, for 2 hours at room temperature. Proteins were visualized by chemiluminescence detection using HRP goat anti-mouse IgG antibody (Cat. No. 405306) at a 1:3000 dilution. Equal MEK1 loading was confirmed by probing membranes with an anti-pan MEK1 antibody. Lane M: Molecular Weight marker.
  • A16117B_Purified_MEK1_Phospho_Antibody_050719.png
    Whole cell extracts (15 µg protein) from HeLa cells untreated (-) or treated (+) with 300 ng/mL nocodazole overnight were resolved on a 4-12% Bis-Tris gel, transferred to nitrocellulose and probed with 0.0625 µg/mL (1:8000 dilution) of purified anti-MEK1 Phospho (Ser298) Antibody, clone A16117B, for 2 hours at room temperature. Proteins were visualized by chemiluminescence detection using HRP goat anti-mouse IgG antibody (Cat. No. 405306) at a 1:3000 dilution. Equal MEK1 loading was confirmed by probing membranes with an anti-pan MEK1 antibody. Lane M: Molecular Weight marker.
  • A16117B_Purified_MEK1_Phospho_Antibody_2_012920
    Human peripheral blood lymphocytes were stimulated with (filled histogram) or without (open histogram) Cell Activation Cocktail (without Brefeldin A) for 15 minutes, then fixed with Fixation Buffer, permeabilized with True-Phos™ Perm Buffer, and intracellularly stained with anti-MEK1 Phospho (Ser298) Antibody (clone A16117B) Purified followed by Goat anti-mouse IgG (minimal x-reactivity) Antibody (clone Poly4053) PE.
  • A16117B_Purified_MEK1_Phospho_Antibody_3_012920
    HELA cells were stimulated with (filled histogram) or without (open histogram) Calyculin A for 15 minutes, then fixed with Fixation Buffer, permeabilized with True-Phos™ Perm Buffer, and intracellularly stained with anti-MEK1 Phospho (Ser298) Antibody (clone A16117B) Purified followed by Goat anti-mouse IgG (minimal x-reactivity) Antibody (clone Poly4053) PE.
Cat # Size Price Quantity Check Availability Save
610601 25 µg 152,00€
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610602 100 µg 360,00€
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Description

MEK1 and MEK2 (Map/Erk Kinase) are serine/threonine kinases in the MAPK/ERK signaling axis that play a critical role in cellular proliferation, differentiation, and migration. Phosphorylation at MEK1 (Ser298) by PAK1 enhances the ability of upstream Raf kinases to phosphorylate regulatory serines (Ser218 and Ser222) in the activation loop, thereby stimulating kinase activity.

Product Details
Technical Data Sheet (pdf)

Product Details

Reactivity
Human, Mouse
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Human MEK1 peptide phosphorylated at Ser298. Complete Freund’s adjuvant.
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/mL
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

WB - Quality tested
ICFC - Verified

Recommended Usage

Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 0.0625 - 1.0 µg per mL. For flow cytometry using our True-Phos™ Perm Buffer, the suggested use of this reagent is ≤ 0.125 µg per million cells in 100 µL volume. It is recommended that the reagent be titrated for optimal performance for each application.

RRID
AB_2810657 (BioLegend Cat. No. 610601)
AB_2810658 (BioLegend Cat. No. 610602)

Antigen Details

Structure
MEK1 is a 393 amino acid protein with a predicted molecular weight of 43 kD
Distribution

Ubiquitously expressed/cytosolic

Biology Area
Cell Biology, Cell Proliferation and Viability, Signal Transduction
Molecular Family
Phospho-Proteins, Protein Kinases/Phosphatase
Antigen References
  1. Song C, et al. 2017. Sci Rep. 7:46833.
  2. Jeong DE, et al. 2017. EMBO J. 36:1046.
  3. Kyriakakis E, et al. 2017. Cell Signal. 35:163.
  4. Narbonne P, et al. PLoS Genet. 13:e1006738.
  5. Wu H, et al. 2013. PLoS One. 8:e83737.
  6. Wang Z, et al. 2010. Oncogene. 29:3362.
  7. Moniz S, et al. 2007. Oncogene. 26:6071.
  8. Park ER, et al. 2007. Cell Signal. 7:1488.
Gene ID
5604 View all products for this Gene ID
UniProt
View information about MEK1 Phospho Ser298 on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 1    Revision Date: 05/07/2019

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

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