- Clone
- 8C6 (See other available formats)
- Other Names
- LIM and SH3 domain protein 1, MLN 50, metastatic lymph node gene 50 protein
- Previously
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Covance Catalog# MMS-426P
- Isotype
- Mouse IgG1
- Ave. Rating
- Submit a Review
- Product Citations
- publications
-
Western blot analysis of cell lysates from Daudi (Human), Raw264.7 (Mouse) and UMR106 (Rat) cells using LASP1 Mouse primary antibody (clone 8C6, 1:1000 dilution) and HRP Goat anti-Mouse secondary antibody (Cat. No. 405306, 1:3000 dilution). Direct-Blot™ HRP anti-β-actin (Cat. No. 643807) was used as a loading control (1:8000 dilution). -
Total cell lysates (15 µg total protein) from HEL, A431, A549 and NIH/3T3 cells were resolved by 4-12% Bis-Tris gel electrophoresis, transferred to a nitrocellulose membrane, and probed with 0.5 µg/mL (1:1000 dilution) of Purified anti-LASP1 Antibody, clone 8C6, overnight at 4°C. Proteins were visualized by chemiluminescence detection using HRP goat anti-mouse IgG Antibody (Cat. No. 405306) at a 1:3000 dilution. Direct-Blot™ HRP anti-GAPDH Antibody (Cat. No. 607904) was used as a loading control at a 1:50000 dilution (lower). Lane M: Molecular Weight marker. Cell lysates were loaded in order of increasing LASP1 mRNA expression levels (source: Human Protein Atlas). -
Immunofluorescence of HepG2 cells with (A) mouse IgG1, κ isotype control (Negative, Cat. No. 401402) or (B-D) LASP1 antibody (Clone 8C6). Alexa Fluor® 488 (Green) Goat anti-Mouse IgG (Cat. No. 405319) was used as secondary antibody. Nuclei were counterstained with DAPI (Blue, Cat. No. 422801). The image was captured with a 60X objective using KEYENCE BZ-X700 fluorescence microscope. Exposure time (Seconds) for (A-D) is 1/15. Concentrations for (A, B) is 10 µg/ml, (C) is 4 µg/ml and (D) is 2 µg/ml.
| Cat # | Size | Price | Quantity Avail. | Save | ||
|---|---|---|---|---|---|---|
| 909301 | 100 µL | 344€ | ||||
Lasp-1 (LIM and SH3 domain protein 1) is an actin-binding phosphoprotein expressed in a wide range of cell types rich in F-actin. Lasp-1 is believed to play a role in the reorganization of the actin cytoskeleton, and localizes to sites of cytoskeletal assembly in response to signalling inputs. Lasp-1 binding partners, in addition to actin, include the endocytic proteins dynamin 2, Hip1r and clathrin, and proteins involved in focal adhesion including zyxin and krp1. The functions of Lasp-1 protein are not well defined, but evidence suggests roles in cell migration, tumor formation, and membrane remodeling in the regulation of ion transport.
Product DetailsProduct Details
- Reactivity
- Human, Mouse, Rat, Rabbit
- Antibody Type
- Monoclonal
- Host Species
- Mouse
- Immunogen
- Clone 8C6 was raised against a KLH-coupled peptide from a conserved region of LASP-1.
- Preparation
- The antibody was purified by affinity chromatography.
- Concentration
- 1 mg/ml
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C. Please note the storage condition for this antibody has been changed from -20°C to between 2°C and 8°C. You can also check your vial or your CoA to find the most accurate storage condition for this antibody.
- Application
-
IHC - Quality tested
WB, ICC - Validated - Recommended Usage
-
Each lot of this antibody is quality control tested by immunohistochemistry. For immunohistochemistry, a dilution range of 1:100 - 1:200 is suggested. For Western blotting, the suggested use of this reagent is 0.5 - 1.0 µg per ml (1:1000 - 1:2000 dilution). For immunocytochemistry, a concentration of 10.0 μg/ml (1:100 dilution) is recommended. It is recommended that the reagent be titrated for optimal performance for each application.
- Application Notes
-
This antibody is effective in immunoblotting (WB) and immunohistochemistry (ICC).
Predicted MW = 37 kD; Positive Control: MDCK cell lysate -
Application References
(PubMed link indicates BioLegend citation) -
- Zhang H, et al. 2009. Physiol Genomics. 38:372. (WB, IF)
- Chew CS, et al. 2008. Am J Physiol Gastrointest Liver Physiol. 295:G37. (WB, IF)
- Chew CS, et al. 2000. J Cell Sci. 113:2035.
- Product Citations
- RRID
-
AB_2565095 (BioLegend Cat. No. 909301)
Antigen Details
- Biology Area
- Cell Biology, Cell Motility/Cytoskeleton/Structure, Neuroscience, Neuroscience Cell Markers
- Gene ID
- 3927 View all products for this Gene ID
- UniProt
- View information about LASP1 on UniProt.org
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