Purified anti-LASP1 Antibody (Previously Covance catalog# MMS-426P)

Pricing & Availability
Clone
8C6 (See other available formats)
Other Names
LIM and SH3 domain protein 1, MLN 50, metastatic lymph node gene 50 protein
Previously
Covance Catalog# MMS-426P
Isotype
Mouse IgG1
Ave. Rating
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Product Citations
publications
1_8C6_PURE_LASP1_Antibody_1_WB_112817
Western blot analysis of cell lysates from Daudi (Human), Raw264.7 (Mouse) and UMR106 (Rat) cells using LASP1 Mouse primary antibody (clone 8C6, 1:1000 dilution) and HRP Goat anti-Mouse secondary antibody (Cat. No. 405306, 1:3000 dilution). Direct-Blot™ HRP anti-β-actin (Cat. No. 643807) was used as a loading control (1:8000 dilution).
  • 1_8C6_PURE_LASP1_Antibody_1_WB_112817
    Western blot analysis of cell lysates from Daudi (Human), Raw264.7 (Mouse) and UMR106 (Rat) cells using LASP1 Mouse primary antibody (clone 8C6, 1:1000 dilution) and HRP Goat anti-Mouse secondary antibody (Cat. No. 405306, 1:3000 dilution). Direct-Blot™ HRP anti-β-actin (Cat. No. 643807) was used as a loading control (1:8000 dilution).
  • 2_8C6_PURE_LASP1_Antibody_WB_113018
    Total cell lysates (15 µg total protein) from HEL, A431, A549 and NIH/3T3 cells were resolved by 4-12% Bis-Tris gel electrophoresis, transferred to a nitrocellulose membrane, and probed with 0.5 µg/mL (1:1000 dilution) of Purified anti-LASP1 Antibody, clone 8C6, overnight at 4°C. Proteins were visualized by chemiluminescence detection using HRP goat anti-mouse IgG Antibody (Cat. No. 405306) at a 1:3000 dilution. Direct-Blot™ HRP anti-GAPDH Antibody (Cat. No. 607904) was used as a loading control at a 1:50000 dilution (lower). Lane M: Molecular Weight marker. Cell lysates were loaded in order of increasing LASP1 mRNA expression levels (source: Human Protein Atlas).
  • 3_8C6_PURE_LASP1_Antibody_2_ICC_112817
    Immunofluorescence of HepG2 cells with (A) mouse IgG1, κ isotype control (Negative, Cat. No. 401402) or (B-D) LASP1 antibody (Clone 8C6). Alexa Fluor® 488 (Green) Goat anti-Mouse IgG (Cat. No. 405319) was used as secondary antibody. Nuclei were counterstained with DAPI (Blue, Cat. No. 422801). The image was captured with a 60X objective using KEYENCE BZ-X700 fluorescence microscope. Exposure time (Seconds) for (A-D) is 1/15. Concentrations for (A, B) is 10 µg/ml, (C) is 4 µg/ml and (D) is 2 µg/ml.
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909301 100 µl 344€
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Description

Lasp-1 (LIM and SH3 domain protein 1) is an actin-binding phosphoprotein expressed in a wide range of cell types rich in F-actin. Lasp-1 is believed to play a role in the reorganization of the actin cytoskeleton, and localizes to sites of cytoskeletal assembly in response to signalling inputs. Lasp-1 binding partners, in addition to actin, include the endocytic proteins dynamin 2, Hip1r and clathrin, and proteins involved in focal adhesion including zyxin and krp1. The functions of Lasp-1 protein are not well defined, but evidence suggests roles in cell migration, tumor formation, and membrane remodeling in the regulation of ion transport.

Product Details
Technical Data Sheet (pdf)

Product Details

Reactivity
Human, Mouse, Rat, Rabbit
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Clone 8C6 was raised against a KLH-coupled peptide from a conserved region of LASP-1.
Preparation
The antibody was purified by affinity chromatography.
Concentration
1 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C. Please note the storage condition for this antibody has been changed from -20°C to between 2°C and 8°C. You can also check your vial or your CoA to find the most accurate storage condition for this antibody.
Application

IHC - Quality tested
WB, ICC - Validated

Recommended Usage

Each lot of this antibody is quality control tested by immunohistochemistry. For immunohistochemistry, a dilution range of 1:100 - 1:200 is suggested. For Western blotting, the suggested use of this reagent is 0.5 - 1.0 µg per ml (1:1000 - 1:2000 dilution). For immunocytochemistry, a concentration of 10.0 μg/ml (1:100 dilution) is recommended. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

This antibody is effective in immunoblotting (WB) and immunohistochemistry (ICC).

Predicted MW = 37 kD; Positive Control: MDCK cell lysate

Application References

(PubMed link indicates BioLegend citation)
  1. Zhang H, et al. 2009. Physiol Genomics. 38:372. (WB, IF)
  2. Chew CS, et al. 2008. Am J Physiol Gastrointest Liver Physiol. 295:G37. (WB, IF)
  3. Chew CS, et al. 2000. J Cell Sci. 113:2035.
Product Citations
  1. Howard CM, et al. 2019. Front Oncol. 9:284. PubMed
Publication Library
RRID
AB_2565095 (BioLegend Cat. No. 909301)

Antigen Details

Biology Area
Cell Biology, Cell Motility/Cytoskeleton/Structure, Neuroscience, Neuroscience Cell Markers
Gene ID
3927 View all products for this Gene ID
UniProt
View information about LASP1 on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 4    Revision Date: 11/28/2017

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Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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