Purified anti-human CD328 (Siglec-7) Antibody

Pricing & Availability
Clone
S7.7 (See other available formats)
Other Names
QA79 membrane protein, Adhesion inhibitory receptor molecule 1, p75/AIRM1, D-siglec
Isotype
Mouse IgG1, κ
Ave. Rating
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Product Citations
publications
S7dot7_PE_030510.jpg
Human peripheral blood lymphocytes stained with anti-human CD8 PerCP/Cy5.5 and purified S7.7 conjugated to PE
  • S7dot7_PE_030510.jpg
    Human peripheral blood lymphocytes stained with anti-human CD8 PerCP/Cy5.5 and purified S7.7 conjugated to PE
  • S7dot7_PE_0305102.jpg
    Human peripheral blood monocytes stained with purified S7.7 conjugated to PE
Cat # Size Price Quantity Avail. Save
347702 100 µg 304€
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Description

Siglec-7 is a 75 kD type I transmembrane protein and a member of the family of sialic acid-binding immunoglobulin-like lectins (Siglecs), also known as p75/AIRM1. It is primarily found on NK cells and monocytes. The cytoplasmic domain of Siglec-7 contains immunoreceptor tyrosine-based inhibitory motif (ITIM). CD328 mediates sialic acid-dependent cell-cell binding and functions as an inhibitory receptor of NK cells. CD328 preferentially binds to sialylated glycans with α2,8 disialyl and α2,6 sialyl residues.

Product Details
Technical Data Sheet (pdf)

Product Details

Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Siglec-7 3T3 transfected cells
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

FC - Quality tested
IP - Reported in the literature

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.5 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Application References

(PubMed link indicates BioLegend citation)
  1. Nicoll G, et al. 1999. J. Biol Chem. 274:34089.
RRID
AB_2189411 (BioLegend Cat. No. 347702)

Antigen Details

Structure
Type I transmembrane protein, Siglec family member, 75 kD
Distribution

Monocytes, NK cells

Function
Inhibitory receptor of NK cells
Interaction
After tyrosine phosphorylation interacts with PTPN6 and SHP-1
Ligand/Receptor
Sialylated glycans (with α,8 disialyl and α2,6 sialyl residues)
Bioactivity
Inhibition of signal transduction by recruiting cytoplasmic phosphatases
Cell Type
Monocytes, NK cells
Biology Area
Immunology
Molecular Family
Adhesion Molecules, CD Molecules, Siglec Molecules
Antigen References

1. Avril T, et al. 2006. Infect Immun. 74:4133.
2. Avril T, et al. 2006. J. Leukoc. Biol. 80:787.
3. Orr SJ, et al. 2007. J. Biol Chem. 282:3418.
4. Brunetta E, et al. 2009. Blood 114:3822.

Gene ID
27036 View all products for this Gene ID
UniProt
View information about CD328 on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 1    Revision Date: 11/30/2012

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