Purified anti-human CD283 (TLR3) Antibody

Pricing & Availability
TLR-104 (See other available formats)
Regulatory Status
Other Names
Toll like receptor 3, TLR-3, CD283
Mouse IgG2a, κ
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Product Citations
Western blot analysis of extracts from 293T parent (lane 1) and 293T transfected with human TLR3 (lane 2) using anti-CD283 antibody(TLR-104).
  • TLR-104_CD283_Purified_Antibody_WB_012114.jpg
    Western blot analysis of extracts from 293T parent (lane 1) and 293T transfected with human TLR3 (lane 2) using anti-CD283 antibody(TLR-104).
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315002 100 µg 440€
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Toll-like receptor 3 (TLR3) is a 104 kD protein also known as CD283. It is expressed selectively on the cytoplasmic membrane and intracellularly in dendritic cells. TLR3 is also highly expressed in the placenta, pancreas, heart, liver, lung, and muscle. TLR3 is a pattern recognition receptor that participates in innate immune response to microbial pathogens. The ligands recognized by TLR3 are polyinosine-polycytidylic acid (Poly(I:C)) and dsRNA. TLR3 ligands induce NF-κB activation (via TRIF-linked RIP1/TLR3 interactions) and cytokine production. TLR3 has been shown to interact with a number of proteins including MYD88, TRAF6, TRIAD3, MAP3K7, and TAB2. This antibody is useful for flow cytometry and Western blotting.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Antibody Type
Host Species
Recombinant human Toll-like receptor 3 protein
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
The antibody was purified by affinity chromatography.
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.

ICFC, WB - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by intracellular immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.5 µg per 106 cells in 100 µl volume. For Western blotting, suggested working dilution(s)is 10 µg antibody in 5 ml antibody dilution buffer for each mini-gel. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

Additional reported applications (for the relevant formats) include: Western bloting. The fluorochrome-labeled TLR-104 antibody is useful for intracellular immunofluorescent staining and flow cytometric analysis. For intracellular staining protocol, please visit www.biolegend.com and click on the support tab.

Application References

(PubMed link indicates BioLegend citation)
  1. Fiala M, et al. 2007. P. Natl. Acad. Sci. USA 10.1073/P. Natl. Acad. Sci. USA.0701267104.
  2. Kong KF, et al. 2008. J. Virol. 82:7613. PubMed
  3. Sadik CD, et al. 2009. Nucleic Acids Res. 37:5041. PubMed
Product Citations
  1. Kong K, et al. 2008. J Virol. 82:7613. PubMed
  2. Sadik C, et al. 2009. Nucleic Acids Res. 37:5041. PubMed
AB_2303470 (BioLegend Cat. No. 315002)

Antigen Details

Toll-like receptor family member, type I membrane protein containing multiple leucine repeats and a interleukin-1 receptor like region in the extracellular domain, predicted molecular weight approximately 104 kD

Selective expression in dendritic cells. Highly expressed in placenta, pancreas, also expressed in heart, liver, lung and muscle

Pattern recognition receptor that participates in innate immune response to microbial pathogens. TLR3 ligands induce NF-κB (via TRIF-linked RIP1/TLR3 interactions) and cytokine production. TLR3 stimulation results in the production of the cytokines
Polyinosine-polycytidylic acid (Poly(I:C)), dsRNA
Cell Type
Dendritic cells
Biology Area
Cell Biology, Immunology, Innate Immunity, Neuroinflammation, Neuroscience, Neuroscience Cell Markers
Molecular Family
CD Molecules, Toll Like Receptors
Antigen References

1. Alexopoulou L, et al. 2001. Nature 413:732.
2. Doyle SE, et al. 2002. Immunity 17:251.
3. Meylan E, et al. 2004. Nature Immun. 5:503.
4. Muzio M, et al. 2000. J. Immunol. 164:5998.
5. Rock FL, et al. 1998. Proc. Natl. Acad. Sci. 95:588.

Gene ID
7098 View all products for this Gene ID
View information about CD283 on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 2    Revision Date: 01/21/2014

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*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.


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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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