- W16171A (See other available formats)
- Regulatory Status
- Other Names
- H2A.X Variant Histone, H2A Histone Family Member X, Histone H2A.X, Histone H2AX, H2AFX
- Rat IgG2a, κ
- Ave. Rating
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- Product Citations
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Histone subunit H2A, along with subunits 2B, 3, and 4, make up the core subunits of the nucleosome octomer. An octomer contains two protomers of each subunit tightly wrapped around a ~147 bp segment of DNA. Histones have integral roles in chromatin integrity, genomic stability, and gene regulation. Post-translational modification of histones in response to certain stimuli results in alterations of nucleosomal positioning relative to DNA. Histone H2A.X is a non-allelic variant of Histone 2A that harbors a C-terminal extension and is essential for checkpoint mediated cell cycle arrest and DNA double-stranded break (DSB) repair in response to both endogenous and exogenous agents, as well as meiotic recombination events and immunoglobulin class switching in lymphocytes. Phosphorylation of C-terminal residue serine 139 by ATM (γ-H2A.X) results in the recruitment of DSB-repair machinery. Phopshorylation of H2A.X is also critical for chromatin fragmentation during apoptosis.Product Details
- Human, Mouse
- Antibody Type
- Host Species
- Synthetic human histone H2A.X peptide (127-143) conjugated to KLH.
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
- The antibody was purified by affinity chromatography.
- 0.5 mg/ml
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C.
WB - Quality tested
ICC - Verified
- Recommended Usage
Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 1.0 - 5.0 µg per ml (1:100-1:500 dilution). For immunocytochemistry, a concentration range of 1.0 - 5.0 μg/ml (1:100-1:500 dilution) is recommended. It is recommended that the reagent be titrated for optimal performance for each application.
- Application Notes
This product is a monoclonal antibody raised against the C-terminus of H2A.X (residues 127-143); BioLegend’s existing antibody against H2A.X (Poly6133, cat# 613302) is a polyclonal antibody which was generated against (partial), N-terminal H2A.X.
This clone is not recommended for ChIP (Chromatin Immunoprecipitation) assays (as determined by in-house testing).
AB_2716198 (BioLegend Cat. No. 600201)
AB_2716199 (BioLegend Cat. No. 600202)
- Histone H2A.X is a 143 amino acid protein with a predicted molecular weight of 15.1 kD.
Ubiquitous tissue expression; nuclear localization
- H2A.X, upon phosphorylation, promotes DNA repair and maintains genomic stability. Important for recombination between immunoglobulin switch regions.
- ATM, MDC1, TP53BP1, BRCA1, MRE11, RAD50, NBN
- Biology Area
- Apoptosis/Tumor Suppressors/Cell Death, Cell Biology, Cell Cycle/DNA Replication, Chromatin Remodeling/Epigenetics, DNA Repair/Replication
- Antigen References
- Chen CC, et al. 2017. Proc. Natl. Acad. Sci. 114: 7665.
- Natale F, et al. 2017. Nat. Commun. 8: 15760.
- Bhargava R, et al. 2017. Proc. Natl. Acad. Sci. 114: 728.
- Weyemi U, et al. 2016. Nat. Commun. 7: 10711.
- Rezaeian AH, et al. 2017. Nat. Cell. Biol. 19: 38.
- Horn S, et al. 2015. Biochim. Biophys. Acta. 1853: 2199.
- Reina-San-Martin B, et al. 2003. J. Exp. Med. 197:1767
- Celeste A, et al. 2002. Science 296:922.
- Mannironi C, et al.1989. Nucleic Acids Res. 17:9113.
- Gene ID
- 3014 View all products for this Gene ID
- View information about H2A.X on UniProt.org
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