Purified anti-Fibrinogen Antibody

Pricing & Availability
Clone
G8 1-2 (See other available formats)
Other Names
Fibrinogen Alpha Chain
Isotype
Mouse IgG1, κ
Ave. Rating
Submit a Review
Product Citations
publications
G81-2_PURE_Fibrinogen_Antibody_1_102220
Western blot of purified anti-Fibrinogen antibody (clone G8 1-2). Lane 1, 5: Molecular weight marker; Lane 2: 2 µg of human liver lysate; Lane 3: 20 µg of HepG2 lysate; Lane 4: 20 µg of MCF7 cell lysate. The blot was incubated with 0.2 µg/mL primary antibody overnight at 4°C, followed by incubation with HRP goat anti-mouse IgG antibody (Cat. No. 405306). Enhanced chemiluminescence was used as the detection system. Cell lysates were loaded in order of descending EGFR expression; predicted expression data was obtained from Human Protein Atlas.
  • G81-2_PURE_Fibrinogen_Antibody_1_102220
    Western blot of purified anti-Fibrinogen antibody (clone G8 1-2). Lane 1, 5: Molecular weight marker; Lane 2: 2 µg of human liver lysate; Lane 3: 20 µg of HepG2 lysate; Lane 4: 20 µg of MCF7 cell lysate. The blot was incubated with 0.2 µg/mL primary antibody overnight at 4°C, followed by incubation with HRP goat anti-mouse IgG antibody (Cat. No. 405306). Enhanced chemiluminescence was used as the detection system. Cell lysates were loaded in order of descending EGFR expression; predicted expression data was obtained from Human Protein Atlas.
  • G81-2_PURE_Fibrinogen_Antibody_2_102220
    ICC staining of purified anti-Fibrinogen antibody (clone G8 1-2) on HepG2 cell. The cells were fixed with 4% PFA, permeabilized with a buffer containing 0.1% Triton X-100 and 0.25% BSA, and blocked with 2% normal goat serum and 0.02% BSA. The cells were then incubated with 5 µg/mL primary antibody overnight at 4°C, followed by incubation with 2.5 µg/mL Alexa Fluor® 594 goat anti-mouse IgG for one hour at room temperature. The cells were co-stained with Flash Phalloidin™ Green 488 (Cat. No. 424201). The slide was mounted with fluoromount G with DAPI. The image was captured with a 60X objective. Scale bar: 20 µm.
Cat # Size Price Quantity Avail. Save
871801 25 µg 144€
Check Availability


Need larger quantities of this item?
Request Bulk Quote
871802 100 µg 376€
Check Availability


Need larger quantities of this item?
Request Bulk Quote
Description

Fibrinogen is a glycoprotein that is synthesized by hepatocytes in the liver. Fibrinogen alpha chain (FGA) is the alpha component of the fibrinogen, which is released to plasma after fibrinogen is cleaved by thrombin. Upon vascular injury, FGA forms the fibrin complex with fibrinogen beta (FGB) and fibrinogen gamma (FGG). The complex is one of the primary components of blood clots. 

Product Details
Technical Data Sheet (pdf)

Product Details

Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/mL
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

WB - Quality tested
ICC - Verified

Recommended Usage

Each lot of this antibody is quality control tested by western blotting. For western blotting, the suggested use of this reagent is 0.2 - 1.0 µg/mL.  For immunocytochemistry, the suggested use of this reagent is 1.0 - 5.0 μg/mL. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

This antibody is specific for fibrinogen alpha chain.

RRID
AB_2888778 (BioLegend Cat. No. 871801)
AB_2888778 (BioLegend Cat. No. 871802)

Antigen Details

Structure
FGA is a 866 amino acid protein with predicted molecular mass of ~84 kD and observed molecular mass of ~62 kD.
Distribution

Tissue distribution: Liver, plasma
Cellular distribution: Extracellular, plasma membrane, endoplasmic reticulum

Function
FGA form fibrin complex with fibrinogen beta (FGB) and fibrinogen gamma (FGG). This complex is the primary components for blood clots.
Interaction
Forms homohexamer with 2-3 non-identical chains. Interacts with PLAT
Cell Type
Plasma cells, Platelets
Biology Area
Cell Biology, Immunology, Neuroinflammation
Molecular Family
Adhesion Molecules
Antigen References
1. Yazaki M, et al. 2018. Int. J. Mol. Sci. 19(1)
2. Lebrton A, et al. 2016. Ann. Biol. Clin., Paris. 74(4):405
3. Fish RJ, et al. 2012. Thromb. Haemost. 108(3):419
Gene ID
2243 View all products for this Gene ID
UniProt
View information about Fibrinogen on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 1    Revision Date: 10/22/2020

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

BioLegend Inc., 8999 BioLegend Way, San Diego, CA 92121 www.biolegend.com
Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

ProductsHere
Insert Note Here
Save Close Clear
Lab Timer
Tools
Login / Register
Remember me
Forgot your password? Reset password?
Create an Account