Purified anti-Cytokeratin Antibody

Pricing & Availability
Clone
OSCAR (See other available formats)
Other Names
Osteoclast Associated, Immunoglobulin-Like Receptor, Osteoclast Associated Receptor OSCAR-S1, Osteoclast Associated Receptor OSCAR-S2, Osteoclast-Associated Receptor
Isotype
Mouse IgG2a, κ
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Product Citations
publications
OSCAR_PURE_Cytokeratin_Antibody_IHC-P_060916
IHC staining of anti-Cytokeratin (clone OSCAR) on formalin-fixed paraffin-embedded human bladdar tissue. Following antigen retrieval using BioLegend's Sodium Citrate Retrieval, the tissue was incubated with the primary antibody at 1 µg/ml for 20 minutes at room temperature. BioLegend's Ultra Streptavidin (USA) HRP Detection Kit (Multi-Species) was used for detection followed by hematoxylin.
  • OSCAR_PURE_Cytokeratin_Antibody_IHC-P_060916
    IHC staining of anti-Cytokeratin (clone OSCAR) on formalin-fixed paraffin-embedded human bladdar tissue. Following antigen retrieval using BioLegend's Sodium Citrate Retrieval, the tissue was incubated with the primary antibody at 1 µg/ml for 20 minutes at room temperature. BioLegend's Ultra Streptavidin (USA) HRP Detection Kit (Multi-Species) was used for detection followed by hematoxylin.
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Cat # Size Price Quantity Avail. Save
908205 25 µg 120€
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908204 100 µg 312€
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Description

This antibody has been shown to detect cytokeratins 7, 8, 18, and 19 and may detect other keratins as well. It does not exhibit cross-reactivity with non-epithelial cells or tissues such as glial and other cells in the brain and the central nervous system. This distinguishes it from the pan cytokerain antibodies (clones AE-1 and AE-3). In normal tissues, it does not stain the superficial layers of the epidermis which suggests it does not detect keratin 1 and keratin 10.

Product Details
Technical Data Sheet (pdf)

Product Details

Reactivity
Human, Cross-Reactivity: Dog, Primate
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
This antibody was developed using a crude cytokeratin extract prepared from RT-4 and MCF-7 cells.
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

IHC-P - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by formalin-fixed paraffin-embedded immunohistochemical staining. For immunohistochemistry, a concentration range of 0.5 - 5.0 µg/ml is suggested. It is recommended that the reagent be titrated for optimal performance for each application.

Application References

(PubMed link indicates BioLegend citation)
  1. Bahrami A, et al. 2008. Mod. Pathol. 21(7):795. (IHC) PubMed
  2. Gown AM, et al. 2003. US Canadian Acad. Pathol. Ann. Meeting (IHC)
RRID
AB_2734685 (BioLegend Cat. No. 908205)
AB_2616961 (BioLegend Cat. No. 908204)

Antigen Details

Structure
This antibody recognizes 30 kD proteins.
Distribution

Cytoskeleton and nucleus.

Function
Regulator of osteoclastogenesis which plays an important bone-specific function in osteoclast differentiation.
Biology Area
Cell Biology, Cell Motility/Cytoskeleton/Structure, Neuroscience, Neuroscience Cell Markers
Molecular Family
Intermediate Filaments
Gene ID
NA
UniProt
View information about Cytokeratin on UniProt.org

Related FAQs

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Go To Top Version: 0    Revision Date: 06/10/2016

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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