Purified anti-Cytokeratin, HMW Antibody

Pricing & Availability
Clone
34βE12 (See other available formats)
Other Names
Cytokeratin, high-molecular weight keratin (HMWK), high-molecular weight cytokeratin (HMWCK)
Isotype
Mouse IgG1, κ
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Product Citations
publications
34betaE12_PURE_Cytokeratin_HMW_IHCP_071216
IHC staining of anti-Cytokeratin, HMW antibody (clone 34βE12) on formalin-fixed paraffin-embedded normal human skin. After antigen retrieval using Retrieve-All Antigen Unmasking System 1, the tissue was incubated with the primary antibody at 0.7 µg/ml for 60 minutes at room temperature. BioLegend's Ultra Streptavidin (USA) HRP Detection Kit was used for detection followed by hematoxylin counterstaining.
  • 34betaE12_PURE_Cytokeratin_HMW_IHCP_071216
    IHC staining of anti-Cytokeratin, HMW antibody (clone 34βE12) on formalin-fixed paraffin-embedded normal human skin. After antigen retrieval using Retrieve-All Antigen Unmasking System 1, the tissue was incubated with the primary antibody at 0.7 µg/ml for 60 minutes at room temperature. BioLegend's Ultra Streptavidin (USA) HRP Detection Kit was used for detection followed by hematoxylin counterstaining.
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Cat # Size Price Quantity Avail. Save
916504 100 µg 312€
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Description

This antibody is specific for high molecular weight cytokeratins 1, 5, 10 and 14. Clone 34βE12 is immunoreactive with squamous and adeno-squamous carcinomas. In normal epithelia, the antibody stains stratified epithelia, myoepithelial cells and basal cells in the prostate gland and bronchi. This antibody is also useful in differentiating benign and malignant tumors of the prostate gland.

Product Details
Technical Data Sheet (pdf)

Product Details

Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
This antibody was developed using a solubilized keratin extracted from human stratum corneum.
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

IHC-P - Quality tested
WB - Validated

Recommended Usage

Each lot of this antibody is quality control tested by formalin-fixed paraffin-embedded immunohistochemical staining. For immunohistochemistry, a concentration range of 0.5 - 5.0 μg/ml is suggested. For Western blotting, a concentration range of 1.0 - 10 μg/ml is suggested. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

The Cytokeratin, High Molecular Weight (HMW) antibody (clone 34βE12) reacts primarily with complex epithelium such as squamous, ductal and transitional epithelium and corresponding tumors.

Application References

(PubMed link indicates BioLegend citation)
  1. Gown AM, Vogel AM. 1985. Am. J. Clin. Pathol. 84:413. (IHC)
  2. Gown AM, Vogel AM. 1982. J. Cell Biol. 95:414. (IHC)
RRID
AB_2629548 (BioLegend Cat. No. 916504)

Antigen Details

Distribution

Tissue distribution: skin, prostate or squamous cell carcinoma.
Cellular distribution: cytoplasm.

Biology Area
Cell Biology, Cell Motility/Cytoskeleton/Structure, Neuroscience, Neuroscience Cell Markers
Molecular Family
Intermediate Filaments
Gene ID
NA
UniProt
View information about Cytokeratin on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 1    Revision Date: 07/20/2016

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

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Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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