- 34βE12 (See other available formats)
- Other Names
- Cytokeratin, high-molecular weight keratin (HMWK), high-molecular weight cytokeratin (HMWCK)
- Mouse IgG1, κ
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- Product Citations
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This antibody is specific for high molecular weight cytokeratins 1, 5, 10 and 14. Clone 34βE12 is immunoreactive with squamous and adeno-squamous carcinomas. In normal epithelia, the antibody stains stratified epithelia, myoepithelial cells and basal cells in the prostate gland and bronchi. This antibody is also useful in differentiating benign and malignant tumors of the prostate gland.Product Details
- Antibody Type
- Host Species
- This antibody was developed using a solubilized keratin extracted from human stratum corneum.
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
- The antibody was purified by affinity chromatography.
- 0.5 mg/ml
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C.
IHC-P - Quality tested
WB - Validated
- Recommended Usage
Each lot of this antibody is quality control tested by formalin-fixed paraffin-embedded immunohistochemical staining. For immunohistochemistry, a concentration range of 0.5 - 5.0 μg/ml is suggested. For Western blotting, a concentration range of 1.0 - 10 μg/ml is suggested. It is recommended that the reagent be titrated for optimal performance for each application.
- Application Notes
The Cytokeratin, High Molecular Weight (HMW) antibody (clone 34βE12) reacts primarily with complex epithelium such as squamous, ductal and transitional epithelium and corresponding tumors.
(PubMed link indicates BioLegend citation)
- Gown AM, Vogel AM. 1985. Am. J. Clin. Pathol. 84:413. (IHC)
- Gown AM, Vogel AM. 1982. J. Cell Biol. 95:414. (IHC)
AB_2629548 (BioLegend Cat. No. 916504)
Tissue distribution: skin, prostate or squamous cell carcinoma.
Cellular distribution: cytoplasm.
- Biology Area
- Cell Biology, Cell Motility/Cytoskeleton/Structure, Neuroscience, Neuroscience Cell Markers
- Molecular Family
- Intermediate Filaments
- Gene ID
- View information about Cytokeratin on UniProt.org
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