PerCP/Cyanine5.5 anti-mouse TCR β chain Antibody

Pricing & Availability
Clone
H57-597 (See other available formats)
Regulatory Status
RUO
Other Names
TCR-β chain, TCR-β, β-TCR
Isotype
Armenian Hamster IgG
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Product Citations
publications
H57-597_PerCPCyanine55_TCR-B-chain_Antibody_110118
C57BL/6 splenocytes were stained with CD3e APC and TCR ß chain (clone H57-597) PerCP/Cyanine5.5 (left), or Armenian Hamster IgG PerCP/Cyanine5.5 isotype control (right).
  • H57-597_PerCPCyanine55_TCR-B-chain_Antibody_110118
    C57BL/6 splenocytes were stained with CD3e APC and TCR ß chain (clone H57-597) PerCP/Cyanine5.5 (left), or Armenian Hamster IgG PerCP/Cyanine5.5 isotype control (right).
Compare all formats See PerCP/Cyanine5.5 spectral data
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109227 25 µg 180€
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109228 100 µg 462€
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Description

T cell receptor (TCR) is a heterodimer consisting of an α and a β chain (TCR α/β) or a γ and a δ chain (TCR γ/δ). TCR-β is a member of the immunoglobulin superfamily and a component of the CD3/TCR complex (along with TCR-α). It is expressed on α/β TCR-bearing T cells and thymocytes. The CD3/TCR complex plays a key role in antigen recognition, signal transduction, and T cell activation.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Armenian Hamster
Immunogen
Affinity purified TCR from mouse DO-11.10 cells
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography, and conjugated with PerCP/Cyanine5.5 under optimal conditions.
Concentration
0.2 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is = 0.25 µg per 106 cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

* PerCP/Cyanine5.5 has a maximum absorption of 482 nm and a maximum emission of 690 nm.

Excitation Laser
Blue Laser (488 nm)
Application Notes

H57-597 is a hamster mAb directed to an epitope of the C region of TCR ß chain12. The H57-597 antibody does not cross-react with ?/d TCR-bearing T cells. Immobilized or soluble H57-597 antibody can activate a/ß TCR-bearing T cells. Additional reported applications (for the relevant formats) for this antibody include: immunoprecipitation2, in vitro stimulation2,3, in vivo depletion4-6, and immunohistochemical staining of acetone-fixed frozen sections7,8,9. The Ultra-LEAF™ purified antibody (Endotoxin <0.01 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. No. 109253-109258).

Additional Product Notes
BioLegend is in the process of converting the name PerCP/Cy5.5 to PerCP/Cyanine5.5. The dye molecule remains the same, so you should expect the same quality and performance from our PerCP/Cyanine5.5 products. Contact Technical Service if you have any questions.
Application References

(PubMed link indicates BioLegend citation)
  1. Gascoigne NJ. 1990. J. Biol. Chem. 265:9296.
  2. Kruisbeek A, et al. 1991. In Current Protocols in Immunology. pp. 3.12.1. (Costim IP)
  3. Davenport C, et al. 1995. J. Immunol. 155:3742. (Costim)
  4. Drobyski W, et al. 1996. Blood 87:5355. (Deplete)
  5. Kummer U, et al. 2001. Immunol. Lett. 75:153. (Deplete)
  6. van der Heyde HC, et al. 1995. J. Immunol. 154:3985. (Deplete)
  7. Tomita K, et al. 1999. Genes Dev. 13:1203. (IHC)
  8. Podd BS, et al. 2006. J. Immunol. 176:6532. (IHC)
  9. Ponomarev ED, et al. 2007. J. Immunol. 178:39. (IHC)
  10. Chappaz S, et al. 2007. Blood doi:10.1182/blood-2007-02-074245. (FC) PubMed
  11. Tsukumo S, et al. 2006. J.Immunol. 177:8365. (FC) PubMed
  12. Grégoire C, et al. 1991. Proc. Natl. Acad. Sci USA 88:8077.
Product Citations
  1. Soon MSF, et al. 2020. Nat Immunol. 1.984027778. PubMed
  2. Oh–Hora M, et al. 2019. J Immunol. 202:2616. PubMed
  3. Lang J, et al. 2020. Nat Commun. 11:1338. PubMed
  4. Hutter K, et al. 2022. Front Immunol. 13:967914. PubMed
  5. Weulersse M, et al. 2020. Immunity. 53(4):824-839.e10. PubMed
  6. Shimizu K, et al. 2020. Molecular Cell. 77(5):937-950.e6.. PubMed
  7. Fachi JL et al. 2019. Cell reports. 27(3):750-761 . PubMed
  8. Christian LS, et al. 2021. Cell Reports. 35(6):109118. PubMed
  9. Saleh MM, et al. 2019. Cell Host Microbe. 25:756. PubMed
  10. Kobayashi T, et al. 2019. Cell. 176:982. PubMed
  11. Delacher M, et al. 2021. Immunity. 54(4):702-720.e17. PubMed
  12. Leech JM, et al. 2020. Cell Host & Microbe. 26(6):795-809.e5.. PubMed
  13. Nakamoto N, et al. 2019. Nat Microbiol. 4:492. PubMed
  14. Jtte BB, et al. 2021. iScience. 24(8):102833. PubMed
  15. Webster HC, et al. 2020. J Immunol Methods. 112702:477. PubMed
  16. Bartleson JM, et al. 2020. Nat Immunol. 1384:21. PubMed
  17. Hayatsu N et al. 2017. Immunity. 47(2):268-283 . PubMed
  18. Nakagawa S et al. 2017. Cell host & microbe. 22(5):667-677 . PubMed
  19. Elsner RA, et al. 2020. Cell Reports. 29(9):2796-2809.e5.. PubMed
  20. Zhong J, et al. 2018. Sci Adv. 4:eaas9864. PubMed
  21. Chen JS, et al. 2022. Sci Immunol. 7:eabl5652. PubMed
  22. Ostendorf BN, et al. 2020. Nat Med. 26:1048. PubMed
  23. Kunis G, et al. 2013. Brain. 136:3427. PubMed
  24. Anderson CK et al. 2019. Cell Rep. 27(2):537-548 . PubMed
  25. Ontiveros-Padilla L, et al. 2021. Microorganisms. 9:. PubMed
  26. Dikiy S, et al. 2021. Immunity. 54(5):931-946.e11. PubMed
  27. Ghilas S, et al. 2021. iScience. 24:103059. PubMed
  28. Tong Q, et al. 2022. FEBS Open Bio. 12:1542. PubMed
  29. Hutter K, et al. 2020. FEBS J. . PubMed
  30. Jia Q, et al. 2021. Theranostics. 4699:11. PubMed
  31. Brown CC, et al. 2020. Cell. 179(4):846-863.e24.. PubMed
  32. Tavazoie MF, et al. 2018. Cell. 172:825. PubMed
  33. Krollmann C, et al. 2022. STAR Protoc. 3:101309. PubMed
  34. Dodard G, et al. 2020. Cell Reports. 32(1):107855.. PubMed
  35. Gurusamy M, et al. 2021. Nat Commun. 12:6798. PubMed
  36. Covarrubias AJ, et al. 2020. Nat Metab. 1265:2. PubMed
  37. Huang D, et al. 2020. Nat Commun. 4.520833333. PubMed
  38. Frost JN, et al. 2021. Med (N Y). 2:164. PubMed
  39. Kim M, et al. 2018. Immunity. 49:151. PubMed
  40. Da Mesquita S, et al. 2021. Science Advances. 7(21):. PubMed
  41. Shissler SC, et al. 2020. Sci Rep. 10:8218. PubMed
  42. Bhatt D, et al. 2021. J Exp Med. 218:. PubMed
  43. Asadi Shahmirzadi A, et al. 2020. Cell Metabolism. 32(3):447-456.e6. PubMed
  44. Bajaña S, et al. 2022. iScience. 25:103732. PubMed
  45. Montes de Oca M, et al. 2016. PLoS Pathog. 12: 1005398. PubMed
  46. Ding L et al. 2018. Cell reports. 25(11):2972-2980 . PubMed
  47. Glodde N et al. 2017. Immunity. 47(4):789-802 . PubMed
  48. Sun T, et al. 2017. J Cell Biol. 216:199. PubMed
  49. Pelly V, et al. 2016. Mucosal Immunol. 10.1038/mi.2016.4. PubMed
  50. Nakamura K, et al. 2018. Cancer Cell. 33:634. PubMed
  51. Wang X, et al. 2021. Immunity. 54(6):1123-1136.e8. PubMed
  52. Koyama M et al. 2019. Immunity. 51(5):885-898 . PubMed
  53. Miao Y et al. 2019. Cell. 177(5):1172-1186 . PubMed
  54. Schäfer AL, et al. 2021. Front Immunol. 12:696810. PubMed
  55. Omenetti S, et al. 2019. Immunity. 51:77. PubMed
  56. Amend A, et al. 2021. Int J Mol Sci. 22:. PubMed
RRID
AB_1575176 (BioLegend Cat. No. 109227)
AB_1575173 (BioLegend Cat. No. 109228)

Antigen Details

Structure
Ig superfamily, CD3/TCR complex with CD3 and TCR α subunit
Distribution

Majority of T cells and thymocytes (correlated to differentiation)

Function
Antigen recognition, T cell activation
Ligand/Receptor
Peptide bound-MHC class I and II
Antigen References

1. Davis MM, et al. 1998. Ann. Rev. Immunol. 16:523.
2. Huppa JB, et al. 2003. Nat. Immunol. 4:749.
3. Kubo R, et al. 1989. J. Immunol. 142:2736.

Gene ID
21577 View all products for this Gene ID
UniProt
View information about TCR beta chain on UniProt.org

Related FAQs

How stable is PerCP/Cyanine5.5 tandem as compared to PerCP alone?

PerCP/Cyanine5.5 is quite photostable and also better than PerCP alone in withstanding fixation.

Other Formats

View All TCR β chain Reagents Request Custom Conjugation
Description Clone Applications
APC anti-mouse TCR β chain H57-597 FC
Biotin anti-mouse TCR β chain H57-597 FC, IHC-F
FITC anti-mouse TCR β chain H57-597 FC, IHC-F
PE anti-mouse TCR β chain H57-597 FC
PE/Cyanine5 anti-mouse TCR β chain H57-597 FC
Purified anti-mouse TCR β chain H57-597 FC, CyTOF®, IHC-F, IP, Costim, Depletion
Alexa Fluor® 488 anti-mouse TCR β chain H57-597 FC, IHC-F
Alexa Fluor® 647 anti-mouse TCR β chain H57-597 FC, IHC-F
APC/Cyanine7 anti-mouse TCR β chain H57-597 FC
PE/Cyanine7 anti-mouse TCR β chain H57-597 FC
Alexa Fluor® 700 anti-mouse TCR β chain H57-597 FC
Pacific Blue™ anti-mouse TCR β chain H57-597 FC
Brilliant Violet 421™ anti-mouse TCR β chain H57-597 FC, IHC-F
PerCP/Cyanine5.5 anti-mouse TCR β chain H57-597 FC
Brilliant Violet 570™ anti-mouse TCR β chain H57-597 FC
Brilliant Violet 510™ anti-mouse TCR β chain H57-597 FC, IHC-F
Purified anti-mouse TCR β chain (Maxpar® Ready) H57-597 FC, CyTOF®
Alexa Fluor® 594 anti-mouse TCR β chain H57-597 FC, IHC-F
PE/Dazzle™ 594 anti-mouse TCR β chain H57-597 FC
Brilliant Violet 605™ anti-mouse TCR β chain H57-597 FC
Brilliant Violet 711™ anti-mouse TCR β chain H57-597 FC
APC/Fire™ 750 anti-mouse TCR β chain H57-597 FC
TotalSeq™-A0120 anti-mouse TCR β chain H57-597 PG
Brilliant Violet 785™ anti-mouse TCR β chain H57-597 FC
Brilliant Violet 650™ anti-mouse TCR β chain H57-597 FC
Ultra-LEAF™ Purified anti-mouse TCR β chain H57-597 FC, CyTOF®, IHC-F, IP, Costim, Depletion
TotalSeq™-C0120 anti-mouse TCR β chain H57-597 PG
TotalSeq™-B0120 anti-mouse TCR β chain H57-597 PG
Go To Top Version: 3    Revision Date: 11/01/2018

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

BioLegend Inc., 8999 BioLegend Way, San Diego, CA 92121 www.biolegend.com
Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

  • APC anti-mouse TCR β chain

  • Biotin anti-mouse TCR β chain

  • FITC anti-mouse TCR β chain

  • PE anti-mouse TCR β chain

  • PE/Cyanine5 anti-mouse TCR β chain

  • Purified anti-mouse TCR β chain

  • Alexa Fluor® 488 anti-mouse TCR β chain

  • Alexa Fluor® 647 anti-mouse TCR β chain

  • APC/Cyanine7 anti-mouse TCR β chain

  • PE/Cyanine7 anti-mouse TCR β chain

  • Alexa Fluor® 700 anti-mouse TCR β chain

  • Pacific Blue™ anti-mouse TCR β chain

  • Brilliant Violet 421™ anti-mouse TCR β chain

  • PerCP/Cyanine5.5 anti-mouse TCR β chain

  • Brilliant Violet 570™ anti-mouse TCR β chain

  • Brilliant Violet 510™ anti-mouse TCR β chain

  • Purified anti-mouse TCR β chain (Maxpar® Ready)

  • Alexa Fluor® 594 anti-mouse TCR β chain

  • PE/Dazzle™ 594 anti-mouse TCR β chain

  • Brilliant Violet 605™ anti-mouse TCR β chain

  • Brilliant Violet 711™ anti-mouse TCR β chain

  • APC/Fire™ 750 anti-mouse TCR β chain

  • TotalSeq™-A0120 anti-mouse TCR β chain

  • Brilliant Violet 785™ anti-mouse TCR β chain

  • Brilliant Violet 650™ anti-mouse TCR β chain

  • Ultra-LEAF™ Purified anti-mouse TCR β chain

  • TotalSeq™-C0120 anti-mouse TCR β chain

  • TotalSeq™-B0120 anti-mouse TCR β chain

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