- E13-161.7 (See other available formats)
- Regulatory Status
- Other Names
- Rat IgG2a, κ
- Ave. Rating
- Submit a Review
- Product Citations
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Ly-6A/E is an 18 kD member of the Ly-6 multigene family also known as Sca-1. Ly6A/E is a glycosylphosphatidylinositol (GPI)-linked protein expressed on hematopoietic stem cells. In mice expressing the Ly-6.2 haplotype (e.g., AKR, C57BL, C57BR, DBA/2, SJL, SWR, and 129), Ly-6A/E is also expressed on peripheral B lymphocytes and thymic and peripheral T lymphocytes. Strains expressing the Ly-6.1 haplotype (e.g., BALB/c, CBA, C3H/He, DBA/1, and NZB) have low Ly-6A/E expression on resting peripheral lymphocytes. The expression of Ly-6A/E on lymphocytes is upregulated upon activation from both Ly6.1 and Ly6.2 haplotype mice. Ly-6A/E is thought to be involved in the regulation of both T and B cell responses. The E13-161.7 antibody has been reported to be useful for immunohistochemical staining of frozen sections, immunoprecipitation, flow cytometry, and complement-mediated cytotoxicity.Product Details
- Verified Reactivity
- Antibody Type
- Host Species
- mouse pre-T cells
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
- The antibody was purified by affinity chromatography, and conjugated with PerCP/Cyanine5.5 under optimal conditions.
- 0.2 mg/ml
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
FC - Quality tested
- Recommended Usage
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 µg per 106 cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.
* PerCP/Cyanine5.5 has a maximum absorption of 482 nm and a maximum emission of 690 nm.
- Application Notes
Additional reported applications (for the relevant formats) include: complement-mediated cytoxicity1, immunohistochemical staining of frozen sections2 and Bouin-fixed, paraffin-embedded samples4, immunofluorescence microscopy4, and immunoprecipitation3.
The two Sca-1 recognizing clones D7 and E13-161.7 have been shown to bind distinct epitopes due to the inability of D7 to block the binding of E13-161.7.7
- Additional Product Notes
- BioLegend is in the process of converting the name PerCP/Cy5.5 to PerCP/Cyanine5.5. The dye molecule remains the same, so you should expect the same quality and performance from our PerCP/Cyanine5.5 products. Contact Technical Service if you have any questions.
(PubMed link indicates BioLegend citation)
- Aihara Y, et al. 1986. Eur. J. Immunol. 16:1391. (CMCD)
- Spangrude GJ, et al. 1988. J. Immunol. 141:3697. (IHC)
- van de Rijin M, et al. 1989. P. Natl. Acad. Sci. USA 86:4634. (IP)
- van Bragt MPA, et al. 2005. Biol. Reprod. 73:634. (IHC, IF)
- Rosas M, et al. 2010. J. Leukoc. Biol. 88:169. PubMed
- Felthause O, et al. 2010. Neurosci Lett. 471:179. PubMed
- Bamezai A and Rock KL. 1995. Proc. Natl. Acad. Sci. USA 92:4294.
- Product Citations
AB_893621 (BioLegend Cat. No. 122523)
AB_893617 (BioLegend Cat. No. 122524)
- Ly-6 multigene family, 18 kD
- Hematopoietic stem cells, activated T cells and B cells, subset of resting B cells and T cells
- Regulates B and T cell responses
- Cell Type
- Hematopoietic stem and progenitors, T cells, B cells, Mesenchymal Stem Cells
- Biology Area
- Immunology, Stem Cells
- Antigen References
1. Rock KL, et al. 1989. Immunol. Rev. 111:195.
2. Morrison SJ, et al. 1994. Immunity 1:661.
3. Spangrude GJ, et al. 1988. J. Immunol. 141:3697.
4. Malek T, et al. 1986. J. Exp. Med. 164:709.
- Gene ID
- 110454 View all products for this Gene ID
- View information about Ly-6A/E on UniProt.org
- How stable is PerCP/Cyanine5.5 tandem as compared to PerCP alone?
PerCP/Cyanine5.5 is quite photostable and also better than PerCP alone in withstanding fixation.
|Purified anti-mouse Ly-6A/E (Sca-1)||E13-161.7||FC, IHC-F, IHC-P, IP|
|Biotin anti-mouse Ly-6A/E (Sca-1)||E13-161.7||FC|
|FITC anti-mouse Ly-6A/E (Sca-1)||E13-161.7||FC|
|PE anti-mouse Ly-6A/E (Sca-1)||E13-161.7||FC|
|APC anti-mouse Ly-6A/E (Sca-1)||E13-161.7||FC|
|PE/Cyanine7 anti-mouse Ly-6A/E (Sca-1)||E13-161.7||FC|
|Alexa Fluor® 488 anti-mouse Ly-6A/E (Sca-1)||E13-161.7||FC|
|Alexa Fluor® 647 anti-mouse Ly-6A/E (Sca-1)||E13-161.7||FC|
|Pacific Blue™ anti-mouse Ly-6A/E (Sca-1)||E13-161.7||FC|
|PerCP/Cyanine5.5 anti-mouse Ly-6A/E (Sca-1)||E13-161.7||FC|
|APC/Fire™ 750 anti-mouse Ly-6A/E (Sca-1)||E13-161.7||FC|
|PE/Dazzle™ 594 anti-mouse Ly-6A/E (Sca-1)||E13-161.7||FC|
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Compare Data Across All Formats
This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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Purified anti-mouse Ly-6A/E (Sca-1)
Biotin anti-mouse Ly-6A/E (Sca-1)
FITC anti-mouse Ly-6A/E (Sca-1)
PE anti-mouse Ly-6A/E (Sca-1)
APC anti-mouse Ly-6A/E (Sca-1)
PE/Cyanine7 anti-mouse Ly-6A/E (Sca-1)
Alexa Fluor® 488 anti-mouse Ly-6A/E (Sca-1)
Alexa Fluor® 647 anti-mouse Ly-6A/E (Sca-1)
Pacific Blue™ anti-mouse Ly-6A/E (Sca-1)
PerCP/Cyanine5.5 anti-mouse Ly-6A/E (Sca-1)
APC/Fire™ 750 anti-mouse Ly-6A/E (Sca-1)
PE/Dazzle™ 594 anti-mouse Ly-6A/E (Sca-1)