PerCP/Cyanine5.5 anti-mouse/human CD44 Antibody

Pricing & Availability
Clone
IM7 (See other available formats)
Regulatory Status
RUO
Other Names
Hermes, Pgp-1, H-CAM, HUTCH-1, ECMR III, gp85, Ly-24
Isotype
Rat IgG2b, κ
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Product Citations
publications
IM7_PerCPCyanine55_CD44_Antibody_010319
C57BL/6 mouse splenocytes were stained with CD44 (clone IM7) PerCP/Cyanine5.5 (filled histogram) or rat IgG2b, ? PerCP/Cyanine5.5 isotype control (open histogram).
  • IM7_PerCPCyanine55_CD44_Antibody_010319
    C57BL/6 mouse splenocytes were stained with CD44 (clone IM7) PerCP/Cyanine5.5 (filled histogram) or rat IgG2b, ? PerCP/Cyanine5.5 isotype control (open histogram).
See PerCP/Cyanine5.5 spectral data
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103031 25 µg 152,00€
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103032 100 µg 360,00€
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Description

CD44 is a 80-95 kD glycoprotein also known as Hermes, Pgp1, H-CAM, or HUTCH. It is expressed on all leukocytes, endothelial cells, hepatocytes, and mesenchymal cells. As B and T cells become activated or progress to the memory stage, CD44 expression increases from low or mid levels to high levels. Thus, CD44 has been reported to be a valuable marker for memory cell subsets. High CD44 expression on Treg cells has been associated with potent suppressive function via high production of IL-10. CD44 is an adhesion molecule involved in leukocyte attachment to and rolling on endothelial cells, homing to peripheral lymphoid organs and to the sites of inflammation, and leukocyte aggregation.

Product Details
Technical Data Sheet (pdf)

Product Details

Reactivity
Mouse, Human, Cross-Reactivity: Chimpanzee, Baboon, Cynomolgus, Rhesus, Squirrel Monkey, Horse (Equine), Cattle (Bovine, Cow), Swine (Pig, Porcine), Dog (Canine), Cat (Feline)
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Dexamethasone-induced myeloid leukemia M1 cells
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography, and conjugated with PerCP/Cyanine5.5 under optimal conditions.
Concentration
0.2 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is = 0.25 µg per 106 cells in 100 µl. It is recommended that the reagent be titrated for optimal performance for each application.

* PerCP/Cyanine5.5 has a maximum absorption of 482 nm and a maximum emission of 690 nm.

Application Notes

Clone IM7 has been reported to recognize an epitope common to alloantigens and all isoforms of CD4417,18 that is located between amino acids 145 and 18620. This clone has been verified for immunocytochemistry (ICC) and frozen immunohistochemistry (IHC-F). Additional reported applications (for the relevant formats) include: immunohistochemistry of acetone-fixed frozen sections and formalin-fixed paraffin-embedded sections6,7, complement-mediated cytotoxicity1, immunoprecipitation1,3, and in vivo inhibition of DTH4,5. The Ultra-LEAF™ purified antibody (Endotoxin < 0.01 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. No. 103046, 103065 - 103069).

Cross-reactivity to ferret has been reported by a collaborator, but not verified in house.

Additional Product Notes
BioLegend is in the process of converting the name PerCP/Cy5.5 to PerCP/Cyanine5.5. The dye molecule remains the same, so you should expect the same quality and performance from our PerCP/Cyanine5.5 products. Contact Technical Service if you have any questions.
Application References

(PubMed link indicates BioLegend citation)
  1. Trowbridge IS, et al. 1982. Immunogenetics 15:299. (ICFC, IP, CMCD)
  2. Katoh S, et al. 1994. J. Immunol. 153:3440. (ELISA)
  3. Budd RC, et al. 1987. J. Immunol. 138:3120. (IP)
  4. Camp RL, et al. 1993. J. Exp. Med. 178:497. (Block)
  5. Weiss JM, et al. 1997. J. Cell Biol. 137:1137. (Block)
  6. Frank NY, et al. 2005. Cancer Res. 65:4320. (IHC) PubMed
  7. Cuff CA, et al. 2001. J. Clin. Invest. 108:1031. (IHC)
  8. Lee JW, et al. 2006. Nature Immunol. 8:181.
  9. Zhang N, et al. 2005. J. Immunol. 174:6967. PubMed
  10. Huabiao C, et al. 2005. J. Immunol. 175:591. PubMed
  11. Gui J, et al. 2007. Int. Immunol. 19:1201. PubMed
  12. Wang XY, et al. 2008. Blood 111:2436. PubMed
  13. Kenna TJ, et al. 2008. Blood 111:2091. PubMed
  14. Yamazaki J, et al. 2009. Blood PubMed
  15. Kmieciak M, et al. 2009. J. Transl. Med. 7:89. (FC) PubMed
  16. Chen YW, et al. 2010. Mol. Cancer Ther. 9:2879. PubMed
  17. Zheng Z, et al. 1995. J. Cell. Biol. 130:485.
  18. Wiranowska M, et al. 2010. Int. J. Cancer 127:532.
  19. Hirokawa Y, et al. 2014. Am J Physiol Gastrointerest Liver Physiol. 306:547. PubMed
  20. Sandmaier BM, et al. 1998. Blood 91:3494.
  21. Yang Y, et al. 2015. Hypertension. 65:1047. PubMed
  22. Peterson VM, et al. 2017. Nat. Biotechnol. 35:936. (PG)
Product Citations
  1. Sun Z, et al. 2013. PLoS One. 8:74672. PubMed
  2. Schmitt E, et al. 2013. J Immunol. 191:5640. PubMed
  3. Charlton J, et al. 2015. PLoS One. 10:119200. PubMed
  4. Schaffert S, et al. 2015. J Immunol. 195: 1470-1479. PubMed
  5. Delmas A, et al. 2016. PLoS One. 11: 0163305. PubMed
  6. Nakajima-Adachi H, et al. 2017. PLoS One. 12(2):e0172795. PubMed
  7. Lucas ED, et al. 2020. Cell Reports. 33(2):108258. PubMed
  8. Webb LMC, et al. 2021. Aging Cell. 20:e13295. PubMed
  9. Walsh SM, et al. 2021. eLife. 10:00. PubMed
  10. Hirai T, et al. 2020. Immunity. 54(1):84-98.e5. PubMed
  11. Ringel AE, et al. 2020. Cell. 183(7):1848-1866.e26. PubMed
  12. Paiva RA, et al. 2021. Cell Reports. 35(2):108967. PubMed
  13. Vaena S, et al. 2021. Cell Reports. 35(5):109076. PubMed
  14. Palazon A, et al. 2017. Cancer Cell. . 10.1016/j.ccell.2017.10.003. PubMed
  15. Jungwirth N et al. 2018. Journal of molecular neuroscience : MN. 65(1):60-73 . PubMed
  16. Yu X, et al. 2019. Nat Commun. 10:574. PubMed
  17. Hayatsu N et al. 2017. Immunity. 47(2):268-283 . PubMed
  18. Brown SM et al. 2017. Endocrinology. 158(10):3592-3604 . PubMed
  19. Yue X, et al. 2019. Nat Commun. 10:2011. PubMed
  20. Yadava K et al. 2019. Elife. 8 pii: e44821. PubMed
  21. Toshiro Hirai et al. 2019. Immunity. 50(5):1249-1261 . PubMed
  22. Benci JL et al. 2019. Cell. 178(4):933-948 . PubMed
  23. Amir M, et al. 2018. Cell Rep. 25:3733. PubMed
  24. Ying Zhang et al. 2017. Cancer cell. 32(3):377-391 . PubMed
  25. Nabet BY et al. 2017. Cell. 170(2):352-366 . PubMed
  26. Benci JL et al. 2016. Cell. 167(6):1540-1554 . PubMed
  27. Horkova V, et al. 2020. Cell Reports. 30(5):1504-1514.e7.. PubMed
  28. Han P, et al. 2020. Sci Adv. 6:eaaz1580. PubMed
  29. Qi S, et al. 2020. Theranostics. 10:1814. PubMed
  30. Jing Y, et al. 2020. Sci Adv. 6:eaax9455. PubMed
  31. Fleskens V, et al. 2019. Cell Rep. 26:3600. PubMed
  32. Van Den Eeckhout B, et al. 2020. NPJ Vaccines. 0.252777778. PubMed
  33. Bromley SK, et al. 2020. Cell Reports. 32(9):108085. PubMed
  34. Zhang H, et al. 2020. Cancer Cell. 37(1):37-54.e9.. PubMed
  35. Fernandes RA, et al. 2020. eLife. 9:e58463.. PubMed
  36. Chakraborty P, et al. 2020. Cell Reports. 28(7):1879-1893.e7.. PubMed
  37. Chen H, et al. 2005. J Immunol. 175:591. PubMed
RRID
AB_2076206 (BioLegend Cat. No. 103031)
AB_2076204 (BioLegend Cat. No. 103032)

Antigen Details

Structure
Variable splicing of CD44 gene generates many CD44 isoforms, 80-95 kD
Distribution

All leukocytes, epithelial cells, endothelial cells, hepatocytes, mesenchymal cells

Function
Leukocyte attachment and rolling on endothelial cells, stromal cells and ECM
Ligand/Receptor
Hyaluronan, MIP-1β, fibronectin, collagen
Cell Type
B cells, Endothelial cells, Epithelial cells, Leukocytes, Mesenchymal cells, Mesenchymal Stem Cells, Tregs
Biology Area
Cell Adhesion, Cell Biology, Immunology, Stem Cells
Molecular Family
Adhesion Molecules, CD Molecules
Antigen References

1. Barclay AN, et al. 1997. The Leukocyte Antigen FactsBook Academic Press.
2. Haynes BF, et al. 1991. Cancer Cells 3:347.
3. Goldstein LA, et al. 1989. Cell 56:1063.
4. Mikecz K, et al. 1995. Nat. Med. 1:558.
5. Hegde V, et al. 2008. J. Leukocyte Biol. 84:134.
6. Liu T, et al. 2009. Biol. Direct 4:40.

Gene ID
12505 View all products for this Gene ID 960 View all products for this Gene ID
UniProt
View information about CD44 on UniProt.org

Related FAQs

How stable is PerCP/Cyanine5.5 tandem as compared to PerCP alone?

PerCP/Cyanine5.5 is quite photostable and also better than PerCP alone in withstanding fixation.

Go To Top Version: 4    Revision Date: 01/03/2019

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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