PerCP/Cyanine5.5 anti-mouse CD11c Antibody

Pricing & Availability
Clone
N418 (See other available formats)
Other Names
αX integrin, integrin αX chain, CR4, p150, ITGAX
Isotype
Armenian Hamster IgG
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Product Citations
publications
N418_PerCPCy5dot5_011108
C57BL/6 mouse splenocytes stained with N418 PerCP/Cyanine5.5
  • N418_PerCPCy5dot5_011108
    C57BL/6 mouse splenocytes stained with N418 PerCP/Cyanine5.5
See PerCP/Cyanine5.5 spectral data
Cat # Size Price Quantity Avail. Save
117327 25 µg 160€
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117328 100 µg 440€
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Description

CD11c is a 150 kD glycoprotein also known as αX integrin, CR4, and p150. CD11c forms a αXβ2 heterodimer with β2 integrin (CD18). It is primarily expressed on dendritic cells, NK cells, a subset of intestinal intraepithelial lymphocytes (IEL), and some activated T cells. The αXβ2 integrin plays an important role in cell-cell contact by binding its ligands: iC3b, fibrinogen, and CD54.

Product Details
Technical Data Sheet (pdf)

Product Details

Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Armenian Hamster
Immunogen
Mouse spleen dendritic cells
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography, and conjugated with PerCP/Cyanine5.5 under optimal conditions.
Concentration
0.2 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤1.0 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.
* PerCP/Cyanine5.5 has a maximum absorption of 482 nm and a maximum emission of 690 nm.

Application Notes

Additional reported applications (for the relevant formats) include: immunoprecipitation3, immunohistochemical staining of acetone-fixed frozen sections3, and immunofluorescence microscopy5, 9 (Alexa Fluor® 488 conjugated N418 was used for IHC in frozen sections10).

Additional Product Notes
BioLegend is in the process of converting the name PerCP/Cy5.5 to PerCP/Cyanine5.5. The dye molecule remains the same, so you should expect the same quality and performance from our PerCP/Cyanine5.5 products. Contact Technical Service if you have any questions.
Application References

(PubMed link indicates BioLegend citation)
  1. Granucci F, et al. 1997. J. Immunol. 159:1794.
  2. Stokes RW, et al. 1998. J. Immunol. 160:5514.
  3. Metlay JP, et al. 1990. J. Exp. Med. 171:1753. (IHC, IP)
  4. Ma XT, et al. 2006. Cancer Research 66:1169.
  5. Chin RK, et al. 2006. J. Immunol. 177:290. (IF)
  6. Cervantes-Barragan L, et al. 2007. Blood 109:1131. (FC) PubMed
  7. Turnquist HR, et al. 2007. J. Immunol. 178:7018. (FC) PubMed
  8. Benson MJ, et al. 2007. J. Exp. Med. doi:10.1084/jem.20070719. (FC) PubMed
  9. You Y, et al. 2009. J. Immunol. 182:7343. (IF) PubMed
  10. Roland CL, et al. 2009. Mol. Cancer Res. 8:1761. (IHC, FC) PubMed
  11. Wikstrom M, et al.2006. J. Immunol. 177:913. PubMed
  12. Pericolini E, et al. 2008. J. Leukocyte Biol. 83:1286. PubMed
  13. Randall LM, et al. 2008. Infect. Immun.76:3312. PubMed
  14. Fahlen-Yrild L, et al. 2009. J. Immunol. 183:5032. PubMed
  15. Osterholzer JJ, et al. 2009. J. Immunol. 183:8044. PubMed
  16. Bankoti J, et al. 2010. Toxicol. Sci. 115:422. (FC) PubMed
  17. Eisenach PA, et al. 2010. J Cell Sci. 123:4182. PubMed
  18. Leppin K, et al. 2014. Invest. Ophthalmol. Vis. Sci. 55:3603. PubMed
  19. Sakai F, et al. 2014. PLoS One. 9:105370. PubMed
  20. Gibbins JD, et al. 2014. Blood. 124:2953. PubMed
  21. White CE, et al. 2015. J Immunol. 194:697. PubMed
  22. Lu X, et al. 2015. J Immunol. 194:2011. PubMed
Product Citations
  1. Espinosa-Cueto P, et al. 2017. PLoS One.. 10.1371/journal.pone.0182126. PubMed
  2. Feng J,et al. 2017. Nat Commun. . 10.1038/s41467-017-01056-8. PubMed
  3. Ma W, et al. 2017. Sci Rep. . 10.1038/s41598-017-15661-6. PubMed
  4. Gaya M et al. 2018. Cell. 172(3):517-533 . PubMed
  5. Luo J et al. 2018. Immunity. 49(1):107-119 . PubMed
  6. Tordesillas L, et al. 2018. Nat Commun. 9:5238. PubMed
  7. Zhao J, et al. 2019. Nat Commun. 10:899. PubMed
  8. Kong XF, et al. 2018. Nat Immunol. 19:973. PubMed
  9. Isvoranu G, et al. 2019. Oncol Lett. 17:4197. PubMed
  10. LaFleur MW, et al. 2019. Nat Commun. 10:1668. PubMed
  11. Frisbee AL, et al. 2019. Nat Commun. 10:2712. PubMed
  12. Clemente–Casares X, et al. 2017. Immunity. 47:974. PubMed
  13. Deniset JF et al. 2019. Immunity. 51(1):131-140 . PubMed
  14. Zasłona Z, et al. 2017. Am J Physiol Lung Cell Mol Physiol. 312:L855. PubMed
  15. Speth JM, et al. 2019. JCI Insight. 4:e131340. PubMed
  16. Hurrell BP, et al. 2019. Cell Rep. 29:4509. PubMed
  17. Alikhanyan K, et al. 2020. Immun Inflamm Dis. 8:181. PubMed
  18. Kobayashi T, et al. 2019. Cell. 176:982. PubMed
  19. Horiguchi H, et al. 2019. Genes Dev. 33:1641. PubMed
  20. Bennion BG, et al. 2020. Cell Reports. 31(11):107771. PubMed
  21. Bartolacci C, et al. 2018. Cancer Immunol Res. 1.281944444. PubMed
  22. Peters N, et al. 2009. PLoS One. 5:e1000484. PubMed
  23. Peters N, et al. 2012. J Immunol. 189:4832. PubMed
  24. Vergadi E, et al. 2014. J Immunol. 192:394. PubMed
  25. Liang Y, et al. 2014. J Immunol. 192:1277. PubMed
  26. Ippolito G, et al. 2014. Proc Natl Acad Sci U S A. 111:998. PubMed
  27. Zasłona Z, et al. 2014. J Immunol. 193:4245. PubMed
  28. Blijswijk J, et al. 2015. J Immunol. 194:307. PubMed
  29. Kitur K, et al. 2015. PLoS Pathog. 11:1004820. PubMed
  30. Mooney J, et al. 2015. Sci Rep. 5: 14603. PubMed
  31. Steiger S, et al. 2015. J Immunol. 195: 5495 - 5502. PubMed
  32. Poczobutt J, et al. 2016. J Immunol. 196: 891 - 901. PubMed
  33. Poczobutt J, et al. 2016. J Immunol. 196: 2847 - 2859. PubMed
  34. Spanier J, et al. 2016. Nat Commun. 7:11804. PubMed
  35. Roufaiel M, et al. 2016. Nat Immunol. 10.1038/ni.3564. PubMed
  36. Martins R, et al. 2016. Nat Immunol. 17:1361-1372. PubMed
  37. Lee L, et al. 2016. PLoS One. 11:e0167693. PubMed
  38. Huo M, et al. 2017. FASEB J. 10.1096/fj.201601030R. PubMed
RRID
AB_2129642 (BioLegend Cat. No. 117327)
AB_2129641 (BioLegend Cat. No. 117328)

Antigen Details

Structure
Integrin α-chain, associates with integrin β2 (CD18), 150 kD
Distribution

Dendritic cells, NK cells, intestinal intraepithelial lymphocytes (IEL), some activated T cells

Function
Cellular adhesion
Ligand/Receptor
iC3b, fibrinogen
Cell Type
Dendritic cells, Epithelial cells, NK cells, T cells, Tregs
Biology Area
Cell Adhesion, Cell Biology, Costimulatory Molecules, Immunology, Innate Immunity, Neuroscience, Neuroscience Cell Markers
Molecular Family
Adhesion Molecules, CD Molecules
Antigen References

1. Barclay A, et al. 1997. The Leukocyte Antigen Facts Book Academic Press.
2. Springer TA. 1994. Cell 76:301.
3. Lopez-Rodriguez C, et al. 1996. J. Immunol. 156:3780.

Gene ID
16411 View all products for this Gene ID
UniProt
View information about CD11c on UniProt.org

Related FAQs

How stable is PerCP/Cyanine5.5 tandem as compared to PerCP alone?

PerCP/Cyanine5.5 is quite photostable and also better than PerCP alone in withstanding fixation.

Go To Top Version: 1    Revision Date: 11/30/2012

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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