PE/Cyanine7 anti-mouse TLR4 (CD284)/MD2 Complex Antibody

Pricing & Availability
MTS510 (See other available formats)
Regulatory Status
Other Names
Toll-like Receptor 4/MD2 complex, TLR4-MD2-complex, TLR4/MD2, CD284/MD2
Rat IgG2a, κ
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Product Citations
Mouse TLR4/MD2/CD14 transfected cells were stained with CD284 (clone MTS510) PE/Cyanine7 (red histogram), or rat IgG2a, κ PE/Cyanine7 (blue histogram).
  • MTS510_PECy7_020210
    Mouse TLR4/MD2/CD14 transfected cells were stained with CD284 (clone MTS510) PE/Cyanine7 (red histogram), or rat IgG2a, κ PE/Cyanine7 (blue histogram).
Compare all formats See PE/Cyanine7 spectral data
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117609 25 µg 212€
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117610 100 µg 494€
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Toll-like receptors are highly conserved from Drosophila to humans and share structural and functional similarities. They are type I transmembrane signaling receptors which activate the innate immune system in response to pathogen invasion. So far, at least 13 TLR members have been identified. The secretory protein, MD2, associates with cell-surface bound TLR4 (CD284) on monocytes, B cells and T cells. TLR4 has been recognized as critical for host recognition of bacterial LPS in conjunction with MD2.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Antibody Type
Host Species
Ba/F3 cell line transfected with mouse TLR4 /MD2 complex
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
The antibody was purified by affinity chromatography, and conjugated with PE/Cyanine7 under optimal conditions.
0.2 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.

FC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.25 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Excitation Laser
Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application Notes

Anti-mouse TLR4 clones SA15-21 and MTS510 recognize distinct epitopes and don’t cross block each other.8 Clone MTS510 binds to an epitope of TLR4/MD-2 that is lost after LPS stimulation.8 In addition to flow cytometric uses, the MTS510 antibody has also been used for immunoprecipitation, immunofluorescence, and blocking of LPS-induced stimulation. The LEAF™ purified antibody (Endotoxin <0.1 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. No. 117607). For highly sensitive assays, we recommend Ultra-LEAF™ purified antibody (Cat. No. 117612) with a lower endotoxin limit than standard LEAF™ purified antibodies (Endotoxin <0.01 EU/µg).

Additional Product Notes
BioLegend is in the process of converting the name PE/Cy7 to PE/Cyanine7. The dye molecule remains the same, so you should expect the same quality and performance from our PE/Cyanine7 products. Please contact Technical Service if you have any questions.
Application References

(PubMed link indicates BioLegend citation)
  1. Akashi S, et al. 2000. J. Immunol. 164:3471. (FC, ICFC, IP, Block)
  2. Kawasaki K, et al. 2003. J. Immunol. 170:413. (FC)
  3. Nalbandian G, et al. 2005. J. Immunol. 175:2666. (FC) PubMed
  4. Kanada S, et al. 2011. Blood 117:2211. PubMed
  5. Laplate P, et al. 2011. J. Biol Chem. 286:42494. PubMed
  6. Khan MA, et al. 2006. Infect. Immun. 74:2522. (IF)
  7. Tanigawa T, et al. 2013. Innate Immun. 19:115. PubMed
  8. Akashi S, et al. 2003. J. Exp. Med. 198:1035.
Product Citations
  1. Lancaster GI et al. 2018. Cell metabolism. 27(5):1096-1110 . PubMed
  2. Chen Z, et al. 2019. J Exp Med. 216:152. PubMed
  3. Zanoni I et al. 2017. Immunity. 47(4):697-709 . PubMed
  4. Huggins MA, et al. 2019. Cell Rep. 28:1729. PubMed
  5. Yazji I, et al. 2013. Proc Natl Acad Sci U S A. 110:9451. PubMed
  6. Daringer N, et al. 2015. J Biol Chem. 290:8764. PubMed
  7. Zhang T, et al. 2021. Journal of Neurochemistry. 157(3):611-623. PubMed
AB_2044019 (BioLegend Cat. No. 117609)
AB_2044020 (BioLegend Cat. No. 117610)

Antigen Details

Type I transmembrane protein complex

Monocytes, B cells and T cells

Activates the innate immune system against Gram-negative bacterial pathogens by recognition of LPS
Bacterial LPS
Cell Type
B cells, Dendritic cells, Monocytes, T cells, Tregs
Biology Area
Immunology, Innate Immunity
Molecular Family
CD Molecules, Toll Like Receptors
Antigen References

1. Frleta D, et al. 2003. J. Leukocyte Biol. 74:1064.

Gene ID
21898 View all products for this Gene ID
View information about TLR4 MD2 Complex on

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Go To Top Version: 3    Revision Date: 02/19/2016

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.


*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.


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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

  • Purified anti-mouse TLR4 (CD284)/MD2 Complex

  • Biotin anti-mouse TLR4 (CD284)/MD2 Complex

  • PE anti-mouse TLR4 (CD284)/MD2 Complex

  • PE/Cyanine7 anti-mouse TLR4 (CD284)/MD2 Complex

  • Ultra-LEAF™ Purified anti-mouse TLR4 (CD284)/MD2 Complex

  • TotalSeq™-A0875 anti-mouse TLR4 (CD284)/MD2 Complex


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