PE/Cyanine7 anti-mouse CD279 (PD-1) Antibody

Pricing & Availability
Clone
29F.1A12 (See other available formats)
Regulatory Status
RUO
Other Names
PD-1, Programmed Death-1
Isotype
Rat IgG2a, κ
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Product Citations
publications
29F.1A12_PECy7_1_110210
Con-A and IL-2 stimulated C57BL/6 mouse splenocytes (3 days) were stained with CD3 APC and CD279 (clone 29F.1A12) PE/Cyanine7 (top) or rat IgG2a, κ isotype control PE/Cyanine7 (bottom).
  • 29F.1A12_PECy7_1_110210
    Con-A and IL-2 stimulated C57BL/6 mouse splenocytes (3 days) were stained with CD3 APC and CD279 (clone 29F.1A12) PE/Cyanine7 (top) or rat IgG2a, κ isotype control PE/Cyanine7 (bottom).
  • 29F.1A12_PECy7_2_110210
Compare all formats See PE/Cyanine7 spectral data
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135215 25 µg 208€
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135216 100 µg 556€
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Description

CD279, also known as programmed death-1 (PD-1), is a 50-55 kD glycoprotein belonging to the CD28 family of the Ig superfamily. PD-1 is expressed on activated splenic T and B cells and thymocytes. It is induced on activated myeloid cells as well. PD-1 is involved in lymphocyte clonal selection and peripheral tolerance through binding its ligands, B7-H1 (PD-L1) and B7-DC (PD-L2). It has been reported that PD-1 and PD-L1 interactions are critical to positive selection and play a role in shaping the T cell repertoire. PD-L1 negative costimulation is essential for prolonged survival of intratesticular islet allografts.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
PD-1 cDNA followed by PD-1-Ig fusion protein
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography, and conjugated with PE/Cyanine7 under optimal conditions.
Concentration
0.2 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.25 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Excitation Laser
Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application Notes

Additional reported applications (for the relevant formats) include: immunohistochemical staining of acetone-fixed frozen tissue3, in vivo blocking of PD-1 binding to its ligands2,3, and spatial biology (IBEX)5,6.

Additional Product Notes

BioLegend is in the process of converting the name PE/Cy7 to PE/Cyanine7. The dye molecule remains the same, so you should expect the same quality and performance from our PE/Cyanine7 products. Please contact Technical Service if you have any questions.

Application References

(PubMed link indicates BioLegend citation)
  1. Good-Jacobson KL, et al. 2010. Nat. Immunol. 11:535. (FC) PubMed
  2. Lázár-Molnár E, et al. 2008. Proc. Natl. Acad. Sci. USA 105:2658. (Block)
  3. Liang SC, et al. 2003. Eur. J. Immunol. 33:2706. (FC, IHC, Block)
  4. Tobias J, et al. 2020. Front Immunol. 11:895 (FC, ELISA) PubMed
  5. Radtke AJ, et al. 2020. Proc Natl Acad Sci U S A. 117:33455-65. (SB) PubMed
  6. Radtke AJ, et al. 2022. Nat Protoc. 17:378-401. (SB) PubMed
Product Citations
  1. Logan K Smith et al. 2018. Immunity. 48(2):299-312 . PubMed
  2. Sandu I, et al. 2020. Nat Commun. 11:4454. PubMed
  3. Liu J, et al. 2021. Front Immunol. 12:747730. PubMed
  4. Aggen DH, et al. 2020. Clin Cancer Res. . PubMed
  5. Wang L, et al. 2019. Cell Rep. 29:1848. PubMed
  6. Mogilenko DA, et al. 2020. Immunity. 54(1):99-115.e12. PubMed
  7. Shi B, et al. 2018. J Immunol. 200:586. PubMed
  8. Wei H, et al. 2021. Malar J. 20:89. PubMed
  9. Zhang J, et al. 2021. MedComm (Beijing). 2:256. PubMed
  10. Liang S, et al. 2022. Acta Pharm Sin B. 12:2494. PubMed
  11. Delacher M, et al. 2021. Immunity. 54(4):702-720.e17. PubMed
  12. Browning LM, et al. 2020. Cell Rep. 33:108219. PubMed
  13. Choi H et al. 2019. Cell Rep. 27(3):806-819 . PubMed
  14. Wu J, et al. 2020. Sci Adv. 6:eaba3458. PubMed
  15. Wiede F, et al. 2021. Cancer Discov. Online ahead of print. PubMed
  16. Saumyaa, et al. 2016. J Immunol. 196: 3677 - 3685. PubMed
  17. Kelsey E Sivick et al. 2018. Cell reports. 25(11):3074-3085 . PubMed
  18. Cassidy BR, et al. 2020. J Neuroinflammation. 17:259. PubMed
  19. Saumyaa S, et al. 2013. Infect Immun . 81:3426. PubMed
  20. Grzelak A, et al. 2018. Int J Mol Sci. 19:. PubMed
  21. Yue X, et al. 2019. Nat Commun. 10:2011. PubMed
  22. Srivastava S, et al. 2019. Cancer Cell. 35:489. PubMed
  23. Cervera–Carrascon V, et al. 2020. Oncoimmunology. 9:1761229. PubMed
  24. Watanabe M, et al. 2020. Nat Commun. 4.808333333. PubMed
  25. Mirando AC, et al. 2020. Oncoimmunology. 9:1760685. PubMed
  26. Kennedy EM, et al. 2022. Nat Commun. 13:5907. PubMed
  27. Moore MJ et al. 2018. eLife. 7 pii: e33057. PubMed
  28. Ullrich L, et al. 2021. Front Immunol. 12:729607. PubMed
  29. Moguche AO et al. 2017. Cell host & microbe. 21(6):695-706 . PubMed
  30. Sandu I, et al. 2020. Cell Reports. 32(8):108078. PubMed
  31. Blanchard L, et al. 2022. STAR Protoc. 3:101444. PubMed
  32. Lou Y, et al. 2021. Int J Mol Sci. 22:. PubMed
  33. Dong X, et al. 2022. Front Immunol. 13:896472. PubMed
  34. Stephens WZ, et al. 2021. Cell Rep. 37:109916. PubMed
  35. Zhang YN, et al. 2021. Sci Adv. 7:eabj3107. PubMed
  36. Zeis P, et al. 2020. Immunity. 53:775. PubMed
  37. Burton B, et al. 2014. Nat Commun. 5:4741. PubMed
  38. Yermanos A, et al. 2020. Front Immunol. 1.143055556. PubMed
  39. Skadow M, et al. 2019. J Immunol. 203:370. PubMed
  40. Raju S, et al. 2020. Cell Reports. 29(9):2556-2564.e3.. PubMed
  41. Baumann D, et al. 2020. Nat Commun. 1.969444444. PubMed
  42. Tian D, et al. 2019. Nat Commun. 10:4246. PubMed
  43. Hu J, et al. 2022. J Immunother Cancer. 10:. PubMed
  44. Xu W, et al. 2021. Immunity. 54(3):526-541.e7. PubMed
  45. Srivastava S, et al. 2020. Cancer Cell. 39(2):193-208.e10. PubMed
  46. Sheriff L, et al. 2022. STAR Protoc. 11:. PubMed
  47. NB M, et al. 2017. J Immunol. 198:1142-1155. PubMed
  48. Guo Y, et al. 2021. Nat Immunol. 22:746. PubMed
  49. Liang Z, et al. 2022. iScience. 25:105233. PubMed
  50. Trindade BC, et al. 2021. Immunity. 54:2273. PubMed
  51. Liu Y, et al. 2021. Nat Commun. 12:6831. PubMed
RRID
AB_10696422 (BioLegend Cat. No. 135215)
AB_10689635 (BioLegend Cat. No. 135216)

Antigen Details

Structure
A 50-55 kD glycoprotein belonging to the CD28 family of the Ig superfamily.
Distribution

Induced on splenic T and B lymphocytes, thymocytes, and myeloid cells after stimulation.

Function
Involved in lymphocyte clonal selection and peripheral tolerance, prolonged survival of allografts.
Ligand/Receptor
B7-H1 (PD-L1) and B7-DC (PD-L2)
Cell Type
B cells, T cells
Biology Area
Cancer Biomarkers, Immunology, Inhibitory Molecules
Molecular Family
CD Molecules, Immune Checkpoint Receptors
Antigen References

1. Nishimura H, et al. 2001. Science 291:319
2. Agata Y, et al. 1996. Int. Immunol. 8:765
3. Liang SC, et al. 2003. Eur. J. Immunol. 33:2706
4. Barber DL, et al. 2006. Nature 439:682
5. Keir ME, et al. 2005. J. Immunol. 175:7372
6. Koehn BH. et al. 2008. J Immunol. 181:5313

Gene ID
18566 View all products for this Gene ID
UniProt
View information about CD279 on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 3    Revision Date: 09/11/2014

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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