PE anti-RPS6 Antibody

Pricing & Availability
Clone
A16009C (See other available formats)
Regulatory Status
RUO
Other Names
40S ribosomal protein S6, Ribosomal Protein S6
Isotype
Mouse IgG2b, κ
Ave. Rating
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Product Citations
publications
A16009C_PE_RPS6_Antibody_051818
HEK 293T cells were fixed, permeabilized, and intracellularly stained with anti-RPS6 (clone A16009C) PE (filled histogram) or purified Mouse IgG2b, κ PE isotype control (open histogram).
  • A16009C_PE_RPS6_Antibody_051818
    HEK 293T cells were fixed, permeabilized, and intracellularly stained with anti-RPS6 (clone A16009C) PE (filled histogram) or purified Mouse IgG2b, κ PE isotype control (open histogram).
See PE spectral data
Cat # Size Price Quantity Check Availability Save
691803 25 tests 184,00€
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691804 100 tests 488,00€
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Description

The eukaryotic ribosome is composed of 40S and 60S subunits, which is involved in the physiological process of protein translation. In many organisms, ribosomal proteins undergo various post-translational modifications, including phosphorylation, acetylation, methylation, O-linked b-N-acetylglucosaminylation, and ubiquitylation. Ribosomal proteinS6 (RPS6), the first identified ribosomal protein which can be phosphorylated, is a component of eukaryotic ribosomal 40S subunit. Functional studies of RPS6 phosphorylation suggested its role in the regulation of protein synthesis, cell proliferation, and glucose homeostasis. The phosphorylation of RPS6 can be triggered by multiple stimuli including growth factors, tumor promoting agents, and mitogens. The inhibition of RPS6 phosphorylation was found mainly under stress conditions such as hypoxia, heat shock, and hyperosmolarity. RPS6 phosphorylation is also commonly used as a marker for neuronal activity and a read out of mammalian target of rapamycin complex 1 (mTORC1) activity.

Product Details
Technical Data Sheet (pdf)

Product Details

Reactivity
Human, Mouse
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Human S6 peptide.
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and 0.2% (w/v) BSA (origin USA).
Preparation
The antibody was purified by affinity chromatography and conjugated with PE under optimal conditions.
Concentration
Lot-specific (please contact technical support for concentration and total µg amount, or use our Lookup tool if you have a lot number.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

ICFC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by intracellular immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.

Excitation Laser
Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
RRID
AB_2734535 (BioLegend Cat. No. 691803)
AB_2734536 (BioLegend Cat. No. 691804)

Antigen Details

Structure
249 amino acids with a predicted molecular weight of 28.7kD.
Distribution

Cytoplasm and nucleus.

Function
Ribosomal protein S6 is the major substrate of protein kinases in eukaryote ribosomes. The phosphorylation is stimulated by growth factors, tumor promoting agents, and mitogens. It is dephosphorylated at growth arrest.
Biology Area
Cell Biology, Signal Transduction
Antigen References

1. Kressler A, et al. 2010. Biochim. Biophys. Acta. 1803:673.
2. Biever A, et al. 2015. Front. Mol. Neurosci. 8:75.
3. Ventimiglia FA, Wool IG. 1974. Proc. Natl. Acad. Sci. USA 71:350.
4. Lastick SM, et al. 1977. Mol. Gen. Genet. 152:223.
5. Ruvinsky I, et al. 2005. Genes Dev. 19:2199.
6. Meyuhas O. 2008. Int. Rev. Cell. Mol. Biol. 268:1.
7. Meyuhas O. 2015. Int. Rev. Cell. Mol. Biol. 320:41.

Gene ID
6194 View all products for this Gene ID
UniProt
View information about RPS6 on UniProt.org

Related FAQs

What type of PE do you use in your conjugates?
We use R-PE in our conjugates.
Go To Top Version: 1    Revision Date: 05/21/2018

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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