PE anti-mouse Ly-6A/E (Sca-1) Recombinant Antibody

Pricing & Availability
Clone
QA17A36 (See other available formats)
Regulatory Status
RUO
Other Names
Sca-1
Isotype
Rat IgG1, κ
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Product Citations
publications
QA17A36_PE_Ly-6A-E_RECOM_Antibody_1_030822_updated
C57BL/6 mouse splenocytes were stained with anti-mouse Ly-6A/E (Sca-1) recombinant (clone QA17A36) PE (filled histogram) or rat IgG2a, κ PE isotype control (open histogram).
  • QA17A36_PE_Ly-6A-E_RECOM_Antibody_1_030822_updated
    C57BL/6 mouse splenocytes were stained with anti-mouse Ly-6A/E (Sca-1) recombinant (clone QA17A36) PE (filled histogram) or rat IgG2a, κ PE isotype control (open histogram).
  • QA17A36_PE_Ly-6A-E_RECOM_Antibody_2_030822_updated
    C57BL/6 mouse bone marrow were stained with anti-mouse Ly-6A/E (Sca-1) recombinant (clone QA17A36) PE (filled histogram) or rat IgG2a, κ PE isotype control (open histogram). Data was gated on the lymphoid population.
See PE spectral data
Cat # Size Price Quantity Check Availability Save
163803 25 µg 176€
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Description

Ly-6A/E, also known as Sca-1, is an 18 kD member of the Ly-6 multigene family. Ly6A/E is a glycosylphosphatidylinositol (GPI)-linked protein expressed on hematopoietic stem cells. In mice expressing the Ly-6.2 haplotype (e.g., AKR, C57BL, C57BR, DBA/2, SJL, SWR, and 129), Ly-6A/E is also expressed on peripheral B lymphocytes and thymic and peripheral T lymphocytes. Strains expressing the Ly-6.1 haplotype (e.g., BALB/c, CBA, C3H/He, DBA/1, and NZB) have low Ly-6A/E expression on resting peripheral lymphocytes. The expression of Ly-6A/E on lymphocytes is upregulated upon activation from both Ly6.1 and Ly6.2 haplotype mice. Ly-6A/E is thought to be involved in the regulation of both T and B cell responses.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Mouse
Antibody Type
Recombinant
Host Species
Rat
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation
The antibody was purified by affinity chromatography and conjugated with PE under optimal conditions.
Concentration
0.2 mg/mL
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 µg per million cells in 100 µL volume. It is recommended that the reagent be titrated for optimal performance for each application.

Excitation Laser
Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
RRID
AB_2922499 (BioLegend Cat. No. 163803)

Antigen Details

Structure
Ly-6 multigene family, 18 kD
Distribution

Hematopoietic stem cells, activated T cells and B cells, subset of resting B cells and T cells

Function
Regulates B and T cell responses
Antigen References
  1. Upadhyay G. 2019. Front Immunol. 10:819.
  2. Morrison SJ, et al. 1994. Immunity. 1:661.
  3. Spangrude GJ, et al. 1988. J Immunol. 141:3697.
Gene ID
110454 View all products for this Gene ID
UniProt
View information about Ly-6A/E on UniProt.org

Related FAQs

What type of PE do you use in your conjugates?
We use R-PE in our conjugates.
Go To Top Version: 1    Revision Date: 03/08/2022

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

  • Purified anti-mouse Ly-6A/E (Sca-1) Recombinant Antibody

  • PE anti-mouse Ly-6A/E (Sca-1) Recombinant Antibody

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