PE anti-mouse/human CD44 Antibody

Pricing & Availability
Clone
IM7 (See other available formats)
Regulatory Status
RUO
Other Names
Hermes, Pgp-1, H-CAM, HUTCH-1, ECMR III, gp85, Ly-24
Isotype
Rat IgG2b, κ
Ave. Rating
Submit a Review
Product Citations
publications
IM7_PE_071608.jpg
C57BL/6 mouse splenocytes stained with IM7 PE
  • IM7_PE_071608.jpg
    C57BL/6 mouse splenocytes stained with IM7 PE
  • IM7_PE_human
    Human peripheral blood lymphocytes stained with IM7 PE
See PE spectral data
Cat # Size Price Quantity Check Availability Save
103023 25 tests 56,00€
Check Availability


Need larger quantities of this item?
Request Bulk Quote
103007 50 µg 72,00€
Check Availability


Need larger quantities of this item?
Request Bulk Quote
103024 100 tests 152,00€
Check Availability


Need larger quantities of this item?
Request Bulk Quote
103008 200 µg 192,00€
Check Availability


Need larger quantities of this item?
Request Bulk Quote
Description

CD44 is a 80-95 kD glycoprotein also known as Hermes, Pgp1, H-CAM, or HUTCH. It is expressed on all leukocytes, endothelial cells, hepatocytes, and mesenchymal cells. As B and T cells become activated or progress to the memory stage, CD44 expression increases from low or mid levels to high levels. Thus, CD44 has been reported to be a valuable marker for memory cell subsets. High CD44 expression on Treg cells has been associated with potent suppressive function via high production of IL-10. CD44 is an adhesion molecule involved in leukocyte attachment to and rolling on endothelial cells, homing to peripheral lymphoid organs and to the sites of inflammation, and leukocyte aggregation.

Product Details
Technical Data Sheet (pdf)

Product Details

Reactivity
Mouse, Human, Cross-Reactivity: Chimpanzee, Baboon, Cynomolgus, Rhesus, Squirrel Monkey, Horse (Equine), Cattle (Bovine, Cow), Swine (Pig, Porcine), Dog (Canine), Cat (Feline)
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Dexamethasone-induced myeloid leukemia M1 cells
Formulation
test sizes: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and 0.2% (w/v) BSA (origin USA).
µg sizes: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography, and conjugated with PE under optimal conditions.
Concentration
µg sizes: 0.2 mg/ml
test sizes: lot-specific (please contact technical support for concentration and total µg amount)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining using the test sizes, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood. For µg sizes, the suggested use of this reagent is ≤0.25 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Excitation Laser
Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application Notes

Clone IM7 has been reported to recognize an epitope common to alloantigens and all isoforms of CD4417,18 that is located between amino acids 145 and 18620. This clone has been verified for immunocytochemistry (ICC) and frozen immunohistochemistry (IHC-F). Additional reported applications (for the relevant formats) include: immunohistochemistry of acetone-fixed frozen sections and formalin-fixed paraffin-embedded sections6,7, complement-mediated cytotoxicity1, immunoprecipitation1,3, and in vivo inhibition of DTH4,5. The Ultra-LEAF™ purified antibody (Endotoxin < 0.01 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. No. 103046, 103065 - 103069).

Cross-reactivity to ferret has been reported by a collaborator, but not verified in house.

Application References

(PubMed link indicates BioLegend citation)
  1. Trowbridge IS, et al. 1982. Immunogenetics 15:299. (ICFC, IP, CMCD)
  2. Katoh S, et al. 1994. J. Immunol. 153:3440. (ELISA)
  3. Budd RC, et al. 1987. J. Immunol. 138:3120. (IP)
  4. Camp RL, et al. 1993. J. Exp. Med. 178:497. (Block)
  5. Weiss JM, et al. 1997. J. Cell Biol. 137:1137. (Block)
  6. Frank NY, et al. 2005. Cancer Res. 65:4320. (IHC) PubMed
  7. Cuff CA, et al. 2001. J. Clin. Invest. 108:1031. (IHC)
  8. Lee JW, et al. 2006. Nature Immunol. 8:181.
  9. Zhang N, et al. 2005. J. Immunol. 174:6967. PubMed
  10. Huabiao C, et al. 2005. J. Immunol. 175:591. PubMed
  11. Gui J, et al. 2007. Int. Immunol. 19:1201. PubMed
  12. Wang XY, et al. 2008. Blood 111:2436. PubMed
  13. Kenna TJ, et al. 2008. Blood 111:2091. PubMed
  14. Yamazaki J, et al. 2009. Blood PubMed
  15. Kmieciak M, et al. 2009. J. Transl. Med. 7:89. (FC) PubMed
  16. Chen YW, et al. 2010. Mol. Cancer Ther. 9:2879. PubMed
  17. Zheng Z, et al. 1995. J. Cell. Biol. 130:485.
  18. Wiranowska M, et al. 2010. Int. J. Cancer 127:532.
  19. Hirokawa Y, et al. 2014. Am J Physiol Gastrointerest Liver Physiol. 306:547. PubMed
  20. Sandmaier BM, et al. 1998. Blood 91:3494.
  21. Yang Y, et al. 2015. Hypertension. 65:1047. PubMed
  22. Peterson VM, et al. 2017. Nat. Biotechnol. 35:936. (PG)
Product Citations
  1. Lee Y, et al. 2012. Nat Immunol. 13:991. PubMed
  2. Blacking T, et al. 2011. Vet Immunol Immunopathol. 141:46. PubMed
  3. Shao W, et al. 2010. Transplant Proc. 42:2777. PubMed
  4. Tyne A, et al. 2013. Vaccine. 31:4322. PubMed
  5. MacDonald K, et al. 2014. J Immunol. 192:3180. PubMed
  6. Davila S, et al. 2014. J Immunol. 192:4284. PubMed
  7. Saenz F, et al. 2014. PLoS One. 9:97666. PubMed
  8. Zhu E, et al. 2014. J Immunol. 192:5599. PubMed
  9. Charlton J, et al. 2015. PLoS One. 10:119200. PubMed
  10. Bonaccorsi-Riani E, et al. 2015. PLoS One. 10: 0136106. PubMed
  11. Olguín J, et al. 2015. Microbes Infect. 17: 586-595. PubMed
  12. Wu Y, et al. 2015. J Immunol. 195: 2612-2623. PubMed
  13. Fiume G, et al. 2015. Sci Rep. 5: 13864. PubMed
  14. MaruYama T, et al. 2015. J Leukoc Biol. 98: 385-393. PubMed
  15. Kaji T, et al. 2016. Int Immunol. 28: 267 - 282. PubMed
  16. Rezende R, et al. 2015. Nat Commun. 6: 8726. PubMed
  17. Kondo M, et al. 2016. J Immunol. 196: 563 - 572. PubMed
  18. Serr I, et al. 2016. Nat Commun. 7:10991. PubMed
  19. Baptista M, et al. 2016. Nat Commun. 7:12175. PubMed
  20. M H, et al. 2016. Open Bio. 6: 150208. PubMed
  21. M H, et al. 2016. Cell Transplant. 25: 811-27. PubMed
  22. Hrdinka M, et al. 2016. PLoS One. 11: 0162863. PubMed
  23. Angela M, et al. 2016. Nat Commun. 7:13683. PubMed
  24. Hammer A, et al. 2016. Proc Natl Acad Sci U S A. 113(49):14109-14114. PubMed
  25. Fan C, et al. 2020. Stem Cell Res Ther. 0.765277778. PubMed
  26. Browning LM, et al. 2020. Cell Rep. 33:108219. PubMed
  27. Shapiro MR, et al. 2020. Front Immunol. 1.972222222. PubMed
  28. Fu X, et al. 2020. Aging (Albany NY). 12:15656. PubMed
  29. Yi X, et al. 2020. Sci Adv. 6:eaba3546. PubMed
  30. Chulpanova DS, et al. 2020. Bioengineering (Basel). 7:00. PubMed
  31. Yermanos A, et al. 2020. Front Immunol. 1.143055556. PubMed
  32. Isakova-Sivak I, et al. 2020. Vaccines (Basel). 8:00. PubMed
  33. Kong T, et al. 2020. Cancer Res. 80:444. PubMed
  34. Girón-Pérez DA, et al. 2020. J Cell Sci. 133:00:00. PubMed
  35. Zhuang Z, et al. 2021. J Exp Med. 218:00:00. PubMed
  36. Lv H, et al. 2020. Cell Metabolism. 33(1):110-127.e5. PubMed
  37. Byun JK, et al. 2020. Molecular Cell. 80(4):592-606.e8. PubMed
  38. Ringel AE, et al. 2020. Cell. 183(7):1848-1866.e26. PubMed
  39. Chakraborty M, et al. 2021. Cell Reports. 34(2):108609. PubMed
  40. Li P, et al. 2017. Oncogenesis. 10.1038/oncsis.2017.24. PubMed
  41. Trial J, et al. 2017. Basic Research in Cardiology. 10.1007/s00395-017-0623-4. PubMed
  42. Pardo E, et al. 2017. PLoS One. 12(6):e0177472. PubMed
  43. Hammer A, et al. 2017. J Neuroinflammation. 10.1186/s12974-017-0954-y. PubMed
  44. Hammer A, et al. 2017. Front Immunol. . 10.3389/fimmu.2017.01922. PubMed
  45. Zhao J, et al. 2018. Stem Cell Reports. 10:180. PubMed
  46. Li P, et al. 2018. J Cell Mol Med. 22:3423. PubMed
  47. Yip HYK, et al. 2018. PLoS One. 3. PubMed
  48. Fan MY et al. 2018. Cell reports. 25(5):1204-1213 . PubMed
  49. Biton M et al. 2018. Cell. 175(5):1307-1320 . PubMed
  50. Wang D, et al. 2018. Immunity. 48:659. PubMed
  51. Lu H, et al. 2019. EBioMedicine. 40:276. PubMed
  52. Miska J et al. 2019. Cell reports. 27(1):226-237 . PubMed
  53. LaFleur MW, et al. 2019. Nat Commun. 10:1668. PubMed
  54. Yue X, et al. 2019. Nat Commun. 10:2011. PubMed
  55. Qi X, et al. 2019. Nat Commun. 10:2141. PubMed
  56. Timilshina M, et al. 2020. Cell Reports. 27(10):2948-2961.e7.. PubMed
  57. Kumar MP, et al. 2018. Cell Rep. 25:1458. PubMed
  58. Yang BH, et al. 2020. Cell Reports. 27(12):3629-3645.e6.. PubMed
  59. Zhang Z, et al. 2019. Cell Death Dis. 10:530. PubMed
  60. Yang C, et al. 2019. Nat Commun. 10:3931. PubMed
  61. Sasaki K, et al. 2019. Nat Commun. 10:3878. PubMed
  62. He W et al. 2018. Immunity. 49(6):1175-1190 . PubMed
  63. Clemente–Casares X, et al. 2017. Immunity. 47:974. PubMed
  64. Shao B, et al. 2019. J Immunol. 10.4049/jimmunol.1900680. PubMed
  65. Bhattacharjee S, et al. 2019. Cell Rep. 28:231. PubMed
  66. Du L et al. 2019. Cell metabolism. 29(6):1363-1375 . PubMed
  67. Santos CP et al. 2019. Nat Commun. 10(1):4407 . PubMed
  68. Kälin S et al. 2017. Cell metabolism. 26(3):475-492 . PubMed
  69. Arima Y et al. 2017. eLife. 6 pii: e25517. PubMed
  70. Velazquez VM, et al. 2017. Mol Ther Methods Clin Dev. 0.277083333. PubMed
  71. Higashi AY, et al. 2019. J Am Soc Nephrol. 30:80. PubMed
  72. Zhang H, et al. 2019. Mol Cell. 76:110. PubMed
  73. Upasani V, et al. 2019. Front Immunol. 2.152777778. PubMed
  74. LaFleur MW, et al. 2019. Nat Immunol. 20:1335. PubMed
  75. Lin W, et al. 2020. Theranostics. 10:4871. PubMed
  76. Shi Y, et al. 2020. Med Sci Monit. 26:e923328. PubMed
  77. Hassan AO, et al. 2020. Cell. 183:169. PubMed
  78. Zhang NN, et al. 2020. Cell. 182(5):1271-1283. PubMed
  79. Yeung F, et al. 2020. Cell Host & Microbe. 27(5):809-822. PubMed
  80. Ge C, et al. 2020. Cell Reports. 29(13):4236-4244.e3.. PubMed
  81. Dong MB, et al. 2020. Cell. 178(5):1189-1204.e23.. PubMed
  82. Hu Q, et al. 2018. Nat Biomed Eng. 0.660416667. PubMed
  83. Chen H, et al. 2005. J Immunol. 175:591. PubMed
  84. Morley S, et al. 2008. J Immunol. 181:4696. PubMed
  85. Chen Y, et al. 2010. Mol Cancer Ther. 2.374305556. PubMed
  86. Sentman A 2011. Blood. 117:6571. PubMed
RRID
AB_493686 (BioLegend Cat. No. 103023)
AB_312958 (BioLegend Cat. No. 103007)
AB_493687 (BioLegend Cat. No. 103024)
AB_312959 (BioLegend Cat. No. 103008)

Antigen Details

Structure
Variable splicing of CD44 gene generates many CD44 isoforms, 80-95 kD
Distribution

All leukocytes, epithelial cells, endothelial cells, hepatocytes, mesenchymal cells

Function
Leukocyte attachment and rolling on endothelial cells, stromal cells and ECM
Ligand/Receptor
Hyaluronan, MIP-1β, fibronectin, collagen
Cell Type
B cells, Endothelial cells, Epithelial cells, Leukocytes, Mesenchymal cells, Mesenchymal Stem Cells, Tregs
Biology Area
Cell Adhesion, Cell Biology, Immunology, Stem Cells
Molecular Family
Adhesion Molecules, CD Molecules
Antigen References

1. Barclay AN, et al. 1997. The Leukocyte Antigen FactsBook Academic Press.
2. Haynes BF, et al. 1991. Cancer Cells 3:347.
3. Goldstein LA, et al. 1989. Cell 56:1063.
4. Mikecz K, et al. 1995. Nat. Med. 1:558.
5. Hegde V, et al. 2008. J. Leukocyte Biol. 84:134.
6. Liu T, et al. 2009. Biol. Direct 4:40.

Gene ID
12505 View all products for this Gene ID 960 View all products for this Gene ID
UniProt
View information about CD44 on UniProt.org

Related FAQs

What type of PE do you use in your conjugates?
We use R-PE in our conjugates.
Go To Top Version: 4    Revision Date: 10/15/2015

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

BioLegend Inc., 8999 BioLegend Way, San Diego, CA 92121 www.biolegend.com
Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

ProductsHere
Insert Note Here
Save Close Clear
Lab Timer
Tools
Login / Register
Remember me
Forgot your password? Reset password?
Create an Account