PE anti-mouse CD178 (FasL) Antibody

Pricing & Availability
Clone
MFL3 (See other available formats)
Regulatory Status
RUO
Other Names
Fas Ligand, FasL, Apo-1 Ligand, CD95 Ligand, TNFSF6
Isotype
Armenian Hamster IgG
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Product Citations
publications
MFL3_PE_090507
Mouse Fas Ligand transfected cells stained with MFL3 PE
  • MFL3_PE_090507
    Mouse Fas Ligand transfected cells stained with MFL3 PE
See PE spectral data
Cat # Size Price Quantity Check Availability Save
106605 50 µg 144,00€
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106606 200 µg 400,00€
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Description

CD178 is a 40 kD member of the TNF/NGF superfamily also known as Fas ligand, FasL, Apo-1 ligand, and CD95 ligand. Cell surface CD178 is expressed on activated T cells and in testis and eye. CD178 is upregulated in activated T cells upon TCR re-engagement and has been shown to induce autocrine and paracrine T cell death. CD178 expression in the eye and testis has been shown to participate in immune privilege at these sites. CD178 can be cleaved from the surface by metalloproteases, and "soluble" CD178 may block the activities of membrane-bound CD178. CD178 binds to CD95 (Fas) to induce apoptotic cell death implicated in the maintenance of peripheral tolerance. CD178/CD95 interactions have also been implicated in the proliferation of CD8+ cells and neutrophil extravasation, chemotaxis, and survival. The MFL3 antibody recognizes CD178 in a wide array of mouse strains and has been reported to block CD178/CD95 induced apoptosis.

Product Details
Technical Data Sheet (pdf)

Product Details

Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Armenian Hamster
Immunogen
B6 mouse FasL cDNA-transfected baby hamster kidney (B6 FasL/BHK) cells
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography, and conjugated with PE under optimal conditions.
Concentration
0.2 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 µg per 106 cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Excitation Laser
Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application Notes

Additional reported applications (for the relevant formats) include: inhibition of the cytotoxicity1-3 and immunofluorescence microscopy4. Fas Ligand is expressed at low density on activated cells. For most successful immunofluorescent staining results, it may be important to maximize signal over background by using a relatively bright fluorochrome-antibody conjugate (Cat. No. 106606) or by using a high sensitivity, three-layer staining technique (e.g., including a biotinylated antibody (Cat. No. 106604) or biotinylated anti-Armenian hamster IgG (Cat. No. 405501) second step, followed by SAv-PE (Cat. No. 405204). The Ultra-LEAF™ purified antibody (Endotoxin < 0.01 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. Nos. 106612).

Application References

(PubMed link indicates BioLegend citation)
  1. Fuller CL, et al. 1999. J. Immunol. 162:6337.
  2. Kayagaki N, et al. 1997. P. Natl. Acad. Sci. USA 94:3914.
  3. Seko Y, et al. 2002. J. Am. Coll.Cardiol. 39:1399.
  4. Park H, et al. 2005. J. Immunol. 175:7193.
  5. Lundgvist A, et al. 2009. Blood 113:6120. PubMed
  6. Oida T, et al. 2011. PLoS ONE 6(4):e18365. (Block) PubMed.
  7. Oura R, et al. 2013. J. Immunol. 190:578. PubMed.
Product Citations
  1. Timilshina M, et al. 2020. Cell Reports. 27(10):2948-2961.e7.. PubMed
  2. Donado CA, et al. 2020. Cell Reports. 31(1):107466. PubMed
  3. Daglas M, et al. 2019. Cell Rep. 29:1178. PubMed
  4. Castillo–Dela Cruz P, et al. 2020. Cell Reports. 29(8):2270-2283.e7.. PubMed
  5. Jain A, et al. 2020. Nat Immunol. 0.920138889. PubMed
  6. Oida T, Weiner H 2010. J Immunol Methods. 362:195. PubMed
  7. Huang J, et al. 2014. J Immunol. 192:1972. PubMed
  8. Schleicher R, et al. 2015. Blood. 126: 1483-1493. PubMed
  9. Wallace JG, et al. 2021. J Allergy Clin Immunol. 147:743. PubMed
  10. Daneshmandi S, et al. 2021. Cell Reports. 34(10):108831. PubMed
RRID
AB_313278 (BioLegend Cat. No. 106605)
AB_313279 (BioLegend Cat. No. 106606)

Antigen Details

Structure
TNF superfamily, 40 kD
Distribution

Activated T cells, spleen, testis, eye

Function
Induces apoptosis
Ligand/Receptor
Fas (CD95)
Cell Type
T cells, Tregs
Biology Area
Apoptosis/Tumor Suppressors/Cell Death, Cell Biology, Immunology, Neuroscience
Molecular Family
CD Molecules
Antigen References

1. Barclay A, et al. 1997. The Leukocyte Antigen FactsBook Academic Press.
2. Nagata S. 1999. Annu Rev Genet. 33:209.
3. Takahashi T, et al. 1994. Cell. 76:969.
4. Hill LL, et al. 1999. Science. 285:898.

Gene ID
14103 View all products for this Gene ID
UniProt
View information about CD178 on UniProt.org

Related FAQs

What type of PE do you use in your conjugates?
We use R-PE in our conjugates.
Go To Top Version: 1    Revision Date: 11/30/2012

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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