PE anti-human ZAP70 Phospho (Tyr493) Antibody

Pricing & Availability
Clone
A16043E (See other available formats)
Regulatory Status
RUO
Other Names
SRK, STD, TZK, p Zeta-Chain (TCR) Associated Protein Kinase 70 kD, p 70 KD Zeta-Chain Associated Protein, p Tyrosine-protein kinase ZAP-70
Isotype
Mouse IgG1, κ
Ave. Rating
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Product Citations
publications
A16043E_PE_ZAP70-Phospho_Antibody_1_020419_updated.png
Human peripheral blood lymphocytes were treated with (left), or without (right), hydrogen peroxide for five minutes, fixed with Fixation Buffer (Cat No. 420801), permiabilized with Intracellular Staining Permeabilization Wash Buffer (Cat No. 421002), then intracellularly stained with CD3 Brilliant Violet 421™ and anti-ZAP70 Phospho (Tyr 493) PE (clone A16043E).
  • A16043E_PE_ZAP70-Phospho_Antibody_1_020419_updated.png
    Human peripheral blood lymphocytes were treated with (left), or without (right), hydrogen peroxide for five minutes, fixed with Fixation Buffer (Cat No. 420801), permiabilized with Intracellular Staining Permeabilization Wash Buffer (Cat No. 421002), then intracellularly stained with CD3 Brilliant Violet 421™ and anti-ZAP70 Phospho (Tyr 493) PE (clone A16043E).
  • A16043E_PE_ZAP70-Phospho_Antibody_2_020419_updated.png
    Human peripheral blood lymphocytes were stimulated by CD3 and CD28 cross-linking (left) or unstimulated (right), fixed with Fixation Buffer (Cat. No. 420801), permiabilized with Intracellular Staining Permeabilization Wash Buffer (Cat. No. 421002), then intracellularly stained with CD3 Brilliant Violet 421™ and anti-ZAP70 Phospho (Tyr 493) (clone A16043E) PE. For CD3 and CD28 cross-linking, cells were incubated with Ultra-LEAF™ anti-CD3 (Cat. No. 300437) and LEAF™ anti-CD28 (Cat. No. 302914) on ice for 15 minutes followed by Purified anti-Mouse Ig (Cat. No. 405301) on ice for 15 minutes, and incubated at 37°C for 2 minutes.
See PE spectral data
Cat # Size Price Quantity Check Availability Save
396003 25 tests 200,00€
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396004 100 tests 472,00€
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Description

ZAP70 is a member of the Syk protein tyrosine kinase subfamily that is expressed exclusively in T cells and NK cells that plays an essential role in T cell receptor (TCR) signaling in combination with the Src family kinases, LCK and FYN. Upon TCR stimulation, phosphorylation of CD3 by LCK leads to the recruitment of ZAP70 to the activated receptor. Upon association of ZAP70 with the activated TCR complex, the kinase is phosphorylated on several tyrosine residues, including Tyrosine 493. Phosphorylation of Tyrosine 493, which is found within the activation loop of the protein, results in increased enzymatic activity. ZAP70 subsequently phosphorylates downstream targets, including the adaptor molecules LAT and LCP2, facilitating the recruitment and activation of other signaling molecules, ultimately leading to T cell proliferation, differentiation, and activation. ZAP70 mays also serve as a scaffold for recruiting additional factors to the activated TCR, and can function to regulate TCR-CD247/CD3Z expression at the plasma membrane. ZAP70 deficiency results in severely impaired immune function, while higher levels of ZAP70 expression are associated with certain B cell malignancies. PTN22 is required for the dephosphorylation of ZAP70 Tyrosine 493.

Product Details
Technical Data Sheet (pdf)

Product Details

Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and 0.2% (w/v) BSA (origin USA).
Preparation
The antibody was purified by affinity chromatography and conjugated with PE under optimal conditions.
Concentration
Lot-specific (please contact technical support for concentration and total µg amount, or use our Lookup tool if you have a lot number.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

ICFC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by intracellular immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.

Excitation Laser
Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application Notes

Positive staining is stimulation and donor dependent. Upon stimulation at high concentrations of H202 some staining of B cells can be seen. Staining for ZAP70 Phospho (Tyr493) using clone A16043E can also be successfully performed using the True-Nuclear™ Transcription Buffer Set and True-Phos™ Perm Buffer.

RRID
AB_2801068 (BioLegend Cat. No. 396003)
AB_2801069 (BioLegend Cat. No. 396004)

Antigen Details

Cell Type
NK cells, T cells
Biology Area
Cell Biology, Immunology, Signal Transduction
Molecular Family
Phospho-Proteins, Protein Kinases/Phosphatase, TCRs
Gene ID
7535 View all products for this Gene ID
UniProt
View information about ZAP70 Phospho Tyr493 on UniProt.org

Related FAQs

What type of PE do you use in your conjugates?
We use R-PE in our conjugates.
Go To Top Version: 1    Revision Date: 01/31/2019

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*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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