PE anti-human TRA-2-54 Antibody

Pricing & Availability
TRA-2-54/2J (See other available formats)
Regulatory Status
Other Names
Liver/Bone/Kidney Alkaline Phosphatase
Mouse IgG1, κ
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Product Citations
Human teratocarcinoma cell line NCCIT cells were stained with TRA-2-54 (clone TRA-2-54/2J) PE (filled histogram) or mouse IgG1, κ PE isotype control (open histogram).
  • TRA-2-542J_PE_TRA-2-54_Antibody_FC_070313
    Human teratocarcinoma cell line NCCIT cells were stained with TRA-2-54 (clone TRA-2-54/2J) PE (filled histogram) or mouse IgG1, κ PE isotype control (open histogram).
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358803 25 tests 222€
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TRA-2-54, also known as Liver/Bone/Kidney Alkaline Phosphatase (ALP or AP), is a tissue non-specific isoform of alkaline phosphatase-related antigen. This antigen is highly expressed on undifferentiated human embryonal carcinoma (EC) and embryonic stem (ES) cells, and is also expressed by liver, bone and kidney cells. It has been reported that the expression level of TRA-2-54 is decreased upon differentiation; therefore measurement of TRA-2-54 expression can be used to monitor the differentiation status of pluripotent stem cells.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Antibody Type
Host Species
Human embryonal carcinoma cells
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
The antibody was purified by affinity chromatography and conjugated with PE under optimal conditions.
Lot-specific (to obtain lot-specific concentration, please enter the lot number in our Concentration and Expiration Lookup or Certificate of Analysis online tools.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.

FC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.

Excitation Laser
Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application Notes

Additional reported applications (for relevant formats) include: immunohistochemistry1 and immunofluorescence1.

Application References

(PubMed link indicates BioLegend citation)
  1. Zou XY, et al. 2012. Stem Cell Res. Ther. 3: 43. (IHC, IF)
AB_2562265 (BioLegend Cat. No. 358803)

Antigen Details


Undifferentiated embryonic stem (ES) cells, embryonal carcinoma (EC) cells, most EC cell lines, bone, liver, kidney

Cell Type
Embryonic Stem Cells
Biology Area
Immunology, Stem Cells
Molecular Family
Protein Kinases/Phosphatase
Antigen References

1. Andrews PW, et al. 1996. Int. J. Cancer. 66:806. 
2. Yan X, et al. 2010. Stem Cells Dev. 19:469. 
3. Zou XY, et al. 2012. Stem Cell Res Ther. 3:43.

Gene ID
249 View all products for this Gene ID
View information about TRA-2-54 on

Related FAQs

What type of PE do you use in your conjugates?
We use R-PE in our conjugates.
Go To Top Version: 1    Revision Date: 06/25/2013

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*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.


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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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