PE anti-human CD15 (SSEA-1) Antibody

Pricing & Availability
Clone
HI98 (See other available formats)
Regulatory Status
RUO
Workshop
IV M141
Other Names
Lewis X, 3-FAL, 3-FL, LNFP III, Lex , SSEA-1, X-hapten
Isotype
Mouse IgM, κ
Ave. Rating
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Product Citations
publications
HI98_PE_061807
Human peripheral blood granulocytes stained with HI98 PE
  • HI98_PE_061807
    Human peripheral blood granulocytes stained with HI98 PE
  • HI98_PE_CD15_Antibody_042922
    Pre-lysed human blood leukocytes were stained with CD15 (Clone HI98) PE (filled histogram) or mouse IgM, κ PE Isotype Control (Open Histogram)

    Data was acquired on the Moxi Flow, exported, and processed using FlowJo software.
Compare all formats See PE spectral data
Cat # Size Price Quantity Check Availability Save
301905 25 tests 176€
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301906 100 tests 388€
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Description

CD15 is 3-fucosyl-N-acetyllactosamine (3-FAL), also known as Lewis X, 3-FAL, X-hapten, and SSEA-1. CD15 is expressed on granulocytes and monocytes. It has also been shown to be expressed on Langerhans cells and some malignant cells. CD15 has been implicated in adhesion, as well as chemotaxis, phagocytosis, and bactericidal activity.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human
Reported Reactivity
Chimpanzee
Antibody Type
Monoclonal
Host Species
Mouse
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation
The antibody was purified by affinity chromatography and conjugated with PE under optimal conditions.
Concentration
Lot-specific (to obtain lot-specific concentration, please enter the lot number in our Concentration and Expiration Lookup or Certificate of Analysis online tools.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.

Excitation Laser
Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application Notes

Clone HI98 has been described to specifically react with 3-fucosyl-N-acetyllactosamine (3-FAL), also called X-hapten, SSEA-1, CD15, or Lewis X. Additional reported applications (for the relevant formats) include: immunohistochemical staining of acetone-fixed frozen sections5 and formalin-fixed paraffin-embedded tissue sections.

Application References

(PubMed link indicates BioLegend citation)
  1. Knapp W, et al. Eds. 1989. Leucocyte Typing IV. Oxford University Press. New York.
  2. Schlossman S, et al. Eds. 1995. Leucocyte Typing V. Oxford University Press. New York.
  3. McMichael A, et al. 1987. Leucocyte Typing III. Oxford University Press. New York.
  4. Yoshino N, et al. 2000. Exp. Anim. (Tokyo) 49:97. (FC)
  5. van Baarsen LG, et al. 2014. Arthritis Res. Ther. 16:426. (IHC)
Product Citations
  1. Carlucci PM, et al. 2018. JCI Insight. 3. PubMed
  2. Arce Vargas F et al. 2018. Cancer cell. 33(4):649-663 . PubMed
  3. Guzzi N et al. 2018. Cell. 173(5):1204-1216 . PubMed
  4. Crippa S, et al. 2019. J Clin Invest. 129:1566. PubMed
  5. Alhaj Hussen K, et al. 2017. Immunity. 47:680. PubMed
  6. Fu J et al. 2019. Cell stem cell. 24(2):227-239 . PubMed
  7. Horn LA, et al. 2017. Oncotarget. 8:57964. PubMed
  8. Kuang YL, et al. 2019. Stem Cell Res. 37:101434. PubMed
  9. Nirschl TR, et al. 2020. Am J Clin Exp Urol. 8:48. PubMed
  10. Zhai Y, et al. 2018. Autophagy. 1.714583333. PubMed
  11. Chabi S, et al. 2020. Cell Reports. 29(8):2307-2320.e6.. PubMed
  12. Saraiva DP, et al. 2018. Front Immunol. 2.184027778. PubMed
  13. Collison J, et al. 2015. J Immunol. 195: 1162-1170. PubMed
  14. Assadi G, et al. 2016. PLoS One. 11:e0168276. PubMed
  15. Zhao M, et al. 2021. Frontiers in Cell and Developmental Biology. 9:652972. PubMed
  16. Qu M, et al. 2021. Frontiers in Oncology. 11:659720. PubMed
RRID
AB_314197 (BioLegend Cat. No. 301905)
AB_314198 (BioLegend Cat. No. 301906)

Antigen Details

Structure
Poly-N-acetyllactosamine
Distribution

Neutrophils, eosinophils, monocytes

Function
Adhesion
Cell Type
Embryonic Stem Cells, Eosinophils, Monocytes, Neural Stem Cells, Neutrophils
Biology Area
Cell Biology, Immunology, Innate Immunity, Neuroscience, Neuroscience Cell Markers, Stem Cells
Molecular Family
Adhesion Molecules, CD Molecules
Antigen References

1. Stocks SC, et al. 1990. Biochem. J. 268:275.

Gene ID
2526 View all products for this Gene ID
UniProt
View information about CD15 on UniProt.org

Related FAQs

What type of PE do you use in your conjugates?
We use R-PE in our conjugates.
Go To Top Version: 2    Revision Date: 12/19/2013

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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