Pacific Blue™ anti-mouse CD3 Antibody

Pricing & Availability
Clone
17A2 (See other available formats)
Other Names
T cell antigen receptor complex, T3
Isotype
Rat IgG2b, κ
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Product Citations
publications
17A2_Blue_091207
C57BL/6 mouse splenocytes stained with Pacific Blue™ 17A2
  • 17A2_Blue_091207
    C57BL/6 mouse splenocytes stained with Pacific Blue™ 17A2
See Pacific Blue™ spectral data
Cat # Size Price Quantity Avail. Save
100213 25 µg 136€
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100214 100 µg 312€
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Description

CD3, also known as T3, is a member of the Ig superfamily and primarily expressed on T cells, NK-T cells, and at different levels on thymocytes during T cell differentiation. CD3 is composed of CD3ε, δ, γ and ζ chains. It forms a TCR complex by associating with TCR α/β or γ/δ chains. CD3 plays a critical role in TCR signal transduction, T cell activation, and antigen recognition by binding the peptide/MHC antigen complex

Product Details
Technical Data Sheet (pdf)

Product Details

Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
γδTCR-positive T-T hybridoma D1
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography, and conjugated with Pacific Blue™ under optimal conditions.
Concentration
0.5 mg/ml
Storage & Handling
The CD3 antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤1.0 µg per 106 cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

* Pacific Blue™ has a maximum emission of 455 nm when it is excited at 405 nm. Prior to using Pacific Blue™ conjugate for flow cytometric analysis, please verify your flow cytometer's capability of exciting and detecting the fluorochrome.


Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.

View full statement regarding label licenses
Excitation Laser
Violet Laser (405 nm)
Product Citations
  1. Ma W, et al. 2017. Cell Death & Disease. 10.1038/cddis.2017.47. PubMed
  2. Sitrin J, et al. 2017. J Immunol. 10.4049/jimmunol.1700608. PubMed
  3. Ma W, et al. 2017. Sci Rep. . 10.1038/s41598-017-15661-6. PubMed
  4. Divan A, et al. 2018. PLoS One. 13:e0196893. PubMed
  5. Aurélien Trompette et al. 2018. Immunity. 48(5):992-1005 . PubMed
  6. Baratin M, et al. 2017. Immunity. 47:349. PubMed
  7. Wang X, et al. 2018. Elife. 7:. PubMed
  8. Luo H, et al. 2019. Cell Rep. 26:945. PubMed
  9. Koh KH, et al. 2019. J Clin Invest. 129:1713. PubMed
  10. Choi H et al. 2019. Cell Rep. 27(3):806-819 . PubMed
  11. Lasso P, et al. 2019. Integr Cancer Ther. 18:1534735419848040. PubMed
  12. Bitschar K, et al. 2019. Nat Commun. 10:2730. PubMed
  13. Li C, et al. 2018. Cell. 174:285. PubMed
  14. Schneider C, et al. 2018. Cell. 174:271. PubMed
  15. Cuburu N, et al. 2019. J Immunol. 202:1250. PubMed
  16. Thompson PJ et al. 2019. Cell metabolism. 29(5):1045-1060 . PubMed
  17. García–Rodríguez S, et al. 2018. Sci Rep. 2.664583333. PubMed
  18. Liu QZ, et al. 2018. Front Immunol. 1.131944444. PubMed
  19. Nowlan B, et al. 2019. Haematologica. 105:71. PubMed
  20. van Erp EA, et al. 2019. Viruses. 0.811111111. PubMed
  21. Phalke SP, et al. 2019. PLoS One. 14:e0218827. PubMed
  22. Fernandes RA, et al. 2020. eLife. 9:e58463.. PubMed
  23. Doo DW, et al. 2020. Ther Adv Med Oncol. 12:1758835920913798. PubMed
RRID
AB_493644 (BioLegend Cat. No. 100213)
AB_493645 (BioLegend Cat. No. 100214)

Antigen Details

Structure
Ig superfamily, CD3/TCR, 20 kD
Distribution

Thymocytes (differentiation dependent), mature T cells, NK-T cells

Function
Antigen recognition, TCR signal transduction, T cell activation
Ligand/Receptor
Peptide antigen/MHC-complex
Antigen References

1. Barclay A, et al. 1997. The Leukocyte Antigen FactsBook Academic Press.
2. Davis MM. 1990. Annu. Rev. Biochem. 59:475.
3. Weiss A, et al. 1994. Cell 76:263.

Gene ID
12502 View all products for this Gene ID
UniProt
View information about CD3 on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 1    Revision Date: 11/30/2012

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

BioLegend Inc., 8999 BioLegend Way, San Diego, CA 92121 www.biolegend.com
Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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