- H4A3 (See other available formats)
- P PR-63; BP 473; P P008
- Other Names
- Lysosome-Associated Membrane Protein 1, LGP-120, LAMP-1
- Mouse IgG1, κ
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- Product Citations
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CD107a, also known as Lysosome-Associated Membrane Protein 1 (LAMP-1) or LGP-120, is a 110-140 kD type I membrane glycoprotein. Mature CD107a is heavily glycosylated from a 40 kD core protein. This molecule is located on the luminal side of lysosomes. Upon activation, CD107a is transferred to the cell membrane surface of activated platelets, activated lymphocytes, macrophages, epithelial cells, endothelial cells, and some tumor cells. CD107a has been suggested to play a role in the protection of lysosomal membrane from lysosomal hydrolases which is involved in cell adhesion and regulation of tumor metastasis, and mediates autoimmune disease progression. CD107a is a ligand for galaptin and E-selectin. Surface expression of LAMP-1 has been shown to correlate with CD8+ T cell and NK cell cytotoxicity.Product Details
- Human, African Green, Baboon, Chimpanzee, Cynomolgus, Pigtailed Macaque, Rhesus
- Antibody Type
- Host Species
- Human adult adherent peripheral blood cells
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
- The antibody was purified by affinity chromatography, and conjugated with Pacific Blue™ under optimal conditions. The solution is free of unconjugated Pacific Blue™.
- 0.5 mg/ml
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
FC - Quality tested
- Recommended Usage
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 2.0 µg per 106 cells in 100 µl volume or 100 µl of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.
* Pacific Blue™ has a maximum emission of 455 nm when it is excited at 405 nm. Prior to using Pacific Blue™ conjugate for flow cytometric analysis, please verify your flow cytometer's capability of exciting and detecting the fluorochrome.
Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.
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- Excitation Laser
Violet Laser (405 nm)
- Application Notes
Additional reported applications (for the relevant formats) include: Western blotting8, immunohistochemical staining2, immunofluorescence5,7, and immunoprecipitation5.
This antibody is specific to human LAMP-1. Positive control: Hela cells; LAMP-1 molecular weight appears to be at ~110 kDa on the gel due to high glycosylation.
(PubMed link indicates BioLegend citation)
- Misse D, et al. 1999. Blood 93:2454.
- Furuta K, et al. 2001. Am. J. Pathol. 159:449. (IHC)
- Watanabe A, et al. 2011. J. Biol. Chem. 286:10702. PubMed
- Baron Gaillard CL, et al. 2011. Mol. Cell. Biol. 22:5459. PubMed
- Hauck CR and Meyer TF. 1997. FEBS Lett. 405:86. (IF, IP)
- De Keersmaecker B, et al. 2012. J. Virol. 86:9351. PubMed
- Knodler LA, et al. 2010. P. Natl. Acad. Sci. USA. 107:17733. (IF)
- Oh J, et al. 2000. Hum. Mol. Genet. 9:375. (WB)
- Salio M, et al. 2013 PNAS. 110:4753. PubMed
- Product Citations
AB_2134615 (BioLegend Cat. No. 328623)
AB_2265606 (BioLegend Cat. No. 328624)
- LAMP-1 is a 417 amino acid protein with a molecular mass of 45 kD.
Macrophages, epithelial cells, endothelial cells, some tumor cells; located on the luminal side of lysosomes or on the surface of cell membranes
- Protect lysosomal membrane from lysosomal hydrolases, adhesion
- Cell Type
- Endothelial cells, Epithelial cells, Macrophages
- Biology Area
- Cell Biology, Immunology, Neurodegeneration, Neuroscience, Protein Trafficking and Clearance
- Molecular Family
- Adhesion Molecules, CD Molecules
- Antigen References
1. Sarafian V, et al. 2006. Arch. Dermatol. Res. 298:7381.
2. Schlossman SF, et al. 1995. Leukocyte Typing V:White Cell Differentiation Antigens. New York:Oxford University Press.
3. Sawada R, et al. 1993. J. Biol. Chem. 268:12675.
4. Chen JW, et al. 1988. J. Biol. Chem. 263:8754.
5. Chen JW, et al. 1986. Biochem. Soc. Symp. 51:97112.
- Gene ID
- 3916 View all products for this Gene ID
- View information about CD107a on UniProt.org
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