- Regulatory Status
- Other Names
- IL-1b ELISA kit
- Ave. Rating
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- Product Citations
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The 10- and 20-plate sizes of this assay have been discontinued. The assay will remain available for purchase under the 5-plate catalog number. Please contact your BioLegend sales representative for bulk pricing quotations.
ELISA Development Systems Engineered for Sensitive, Precise and Reliable Serum and Plasma Cytokine Quantitation
BioLegend's ELISA Max™ Sets provide all the time-savings, convenience and performance of a high-end, commercial ELISA kit for a fraction of the price! BioLegend's ELISA Max™ Sets, in two appealing formats, address the need for sensitivity, accuracy, dependability, and versatility of an ELISA development system, while providing maximum cost effectiveness, value, and ease of use.
Both the ELISA Max™ Set Standard and ELISA Max™ Set Deluxe are specifically engineered for accurate recovery and measurement of analytes in complex fluids, such as serum and plasma (as well as activated cell cultures) without any interference by confounding serum factors, such as heterophilic antibody or rheumatoid factor.
- Kit Contents
- Mouse IL-1β ELISA MAX™ Capture Antibody (200X)
- Mouse IL-1β ELISA MAX™ Detection Antibody (200X)
- Mouse IL-1β Standard
- Avidin-HRP (1000X)
- As a part of BioLegend's efforts to go green, each new lot of the ELISA MAX™ sets will include a summarized version of its manual. Click here for more information.
- Application Notes
BioLegend's mouse and human IL-1ß ELISA kits use antibodies raised against the mature form of IL-1ß. However, because pro-IL-1ß contains the mature form, these antibodies likely recognize both the pro- and the mature forms of the protein. The human IL-1ß ELISA kits have been shown to equally recognize both pro- and mature forms of IL-1ß.
(PubMed link indicates BioLegend citation)
- Product Citations
AB_2883996 (BioLegend Cat. No. 432601)
- Standard Range
- 31.3-2,000 pg/mL
- Materials Not Included
- Microwell plates: 96-well Nunc MaxiSorp™ is recommended.
- A microplate reader capable of measuring absorbance at 450 nm
- Adjustable pipettes to measure volumes ranging from 2 µL to 1 mL
- Deionized (DI) water
- Coating Buffer: 8.4 g NaHCO3, 3.56 g Na2CO3, add DI H2O to 1.0 L, pH to 9.5 (BioLegend Cat. No. 421701 is recommended.)
- Assay Diluent: 10% Fetal Bovine Serum or 1% BSA in Phosphate-Buffered Saline (PBS) (BioLegend Cat. No. 421203 is recommended.)
- PBS: 8.0 g NaCl, 1.16 g Na2HPO4, 0.2 g KH2PO4, 0.2 g KCl, add DI water to 1.0 L, pH to 7.4
- Wash Buffer: Phosphate-Buffered Saline (PBS) + 0.05% Tween-20 (BioLegend Cat. No. 421601 is recommended.)
- Wash bottle or automated microplate washer
- TMB Substrate Solution - BioLegend Cat. No. 421101 is recommended.
- Stop Solution (2 N H2SO4)
- Log-Log graph paper or software for data analysis
- Tubes to prepare standard dilutions
- Absorbent paper
- Cell Sources
- Monocytes, tissue macrophages, Langerhan cells, dendritic cells, T lymphocytes, B lymphocytes, natural killer (NK) cells, large granular lymphocytes (LGL), vascular endothelium, smooth muscle, fibroblasts, thymic epithelia, astrocytes, microglia, glioma
- Biology Area
- Cell Biology, Immunology, Innate Immunity, Neuroinflammation, Neuroscience
- Molecular Family
- Gene ID
- 16176 View all products for this Gene ID
- View information about IL-1beta on UniProt.org
- Do your IL-1β ELISA kits detect pro-IL-1β or the mature form?
The antibodies in our IL-1β kits were generated against the mature form of the protein. However, because the pro-form of the protein contains the mature form, the antibodies should detect both forms.