The 10- and 20-plate sizes of this assay have been discontinued. The assay will remain available for purchase under the 5-plate catalog number. Please contact your BioLegend sales representative for bulk pricing quotations.
Mouse IL-10 was originally described as Cytokine Synthesis Inhibitory Factor (CSIF) due to its ability to inhibit cytokine production by TH1 clones. IL-10 is expressed in activated CD8+ and CD4+ T cells, activated monocytes, mast cells, and Ly-1 B cells. IL-10 shares over 80% sequence homology with the Epstein-Barr virus protein BCRFI. The functions of IL-10 include inhibition of macrophage-mediated cytokine synthesis, suppression of the delayed-type hypersensitivity response, and stimulation of the TH2 cell response, which results in elevated antibody production.
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- Kit Contents
- Mouse IL-10 ELISA MAX™ Capture Antibody (200X)
- Mouse IL-10 ELISA MAX™ Detection Antibody (200X)
- Mouse IL-10 Standard
- Avidin-HRP (1000X)
- As a part of BioLegend's efforts to go green, each new lot of the ELISA MAX™ sets will include a summarized version of its manual. Click here for more information.
(PubMed link indicates BioLegend citation)
- Product Citations
- Standard Range
- 31.3-2,000 pg/mL
- Materials Not Included
- Microwell plates: 96-well Nunc MaxiSorp™ is recommended.
- A microplate reader capable of measuring absorbance at 450 nm
- Adjustable pipettes to measure volumes ranging from 2 µL to 1 mL
- Deionized (DI) water
- Coating Buffer: Phosphate-Buffered Saline (PBS), pH 7.4
- Assay Diluent: 10% Fetal Bovine Serum or 1% BSA in Phosphate-Buffered Saline (PBS) (BioLegend Cat. No. 421203 is recommended.)
- PBS: 8.0 g NaCl, 1.16 g Na2HPO4, 0.2 g KH2PO4, 0.2 g KCl, add DI water to 1.0 L, pH to 7.4
- Wash Buffer: Phosphate-Buffered Saline (PBS) + 0.05% Tween-20 (BioLegend Cat. No. 421601 is recommended.)
- Wash bottle or automated microplate washer
- TMB Substrate Solution - BioLegend Cat. No. 421101 is recommended.
- Stop Solution (2 N H2SO4)
- Log-Log graph paper or software for data analysis
- Tubes to prepare standard dilutions
- Absorbent paper
- Cell Sources
- Activated CD8+ T cells, Th0, Th2 subset of CD4+ T cells, Ly-1+ B cells, monocytes, macrophages, keratinocytes
- Cell Type
- Biology Area
- Molecular Family
- Gene ID
- 16153 View all products for this Gene ID
- View information about IL-10 on UniProt.org