- Other Names
- Mouse CD19 Positive Selection, CD19 Positive selection
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- Product Citations
- publications
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A single cell suspension from pooled C57BL/6 mouse lymphoid tissues was prepared to isolate CD19+ B cells using the MojoSort™ Mouse CD19 Nanobeads. Cells were stained with CD45R/B220 (clone RA3-6B2) PE. Dead cells were excluded by 7-AAD. -
| Cat # | Size | Price | Quantity Avail. | Save | ||
|---|---|---|---|---|---|---|
| 480001 | 20 tests | 240€ | ||||
| 480002 | 200 tests | 840€ | ||||
CD19+ expressing cells are either selected or depleted by incubating your sample with the directly conjugated CD19 Nanobeads. The magnetically labeled fraction is retained by the use of a magnetic separator. After collection of the CD19+ cells, downstream applications include functional assays, gene expression, phenotypic characterization, etc.
Product DetailsKit Contents
- Kit Contents
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For Cat# 480001:
- 1 vial containing 200 µl of CD19 Nanobeads.
For Cat# 480002:
- 2 vials containing 1 ml each of CD19 Nanobeads.
Product Details
- Reactivity
- Mouse
- Formulation
- Aqueous solution containing BSA and 0.05% sodium azide.
- Preparation
- Antibody-conjugated particles: protein-coated magnetic nanobeads.
- Storage & Handling
- Mouse CD19 Nanobeads should be stored undiluted between 2°C and 8°C.
- Application
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Cell Separation (MojoSort™) - Quality tested
- Recommended Usage
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10 µl Mouse CD19 Nanobeads for 1x107 cells in 100 µl of buffer.
- Application Notes
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This kit is designed for the positive selection of CD19+ B cells from lymphoid tissues.
Antibody or cocktail dilution to use in column: N/A
Nanobead dilution to use in columns: 10X - Additional Product Notes
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View more applications data for this product in our Scientific Poster Library.
Antigen Details
- Biology Area
- Immunology, Costimulatory Molecules
- Molecular Family
- CD Molecules
- Gene ID
- NA
Related FAQs
- Are MojoSort™ Nanobeads compatible with other commercially available magnetic separation systems?
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MojoSort™ magnetic particles can be used with other commercially available magnetic separators, both free standing magnets and column-based systems. Because MojoSort™ protocols are optimized for the MojoSort™ separator, the protocols may need to be adjusted for other systems. Please contact BioLegend Technical Service for more information and guidance. We do not recommend using MojoSort™ particles for BD’s IMag™ or Life Technologies’ DynaMag™.
- Can your magnetic particles be sterile filtered?
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Yes, they can be sterile filtered as the particles are smaller than 0.22 µm.
- Is there a way to detach your magnetic particles from the cell surface?
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No, not currently. We have found that cells are functional without the need to detach the magnetic Nanobeads.
- What is the size of your magnetic particles?
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The average diameter is approximately 130 nm.
