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CD14+ expressing cells are either selected or depleted by incubating your sample with the directly conjugated CD14 Nanobeads. The magnetically labeled fraction is retained by the use of a magnetic separator. After collection of the CD14+ expressing cells, downstream applications include functional assays, gene expression, phenotypic characterization, etc.Product Details
- Kit Contents
For Cat# 480093:
- 1 vial containing 100 µl of Human TruStain FcX™ (Fc Receptor Blocking Solution)
- 1 vial containing 200 µl of Human CD14 Nanobeads
For Cat# 480094:
- 1 vial containing 1 ml of Human TruStain FcX™ (Fc Receptor Blocking Solution)
- 2 vials containing 1 ml of Human CD14 Nanobeads
- Verified Reactivity
- Aqueous solution containing 0.05% sodium azide and 0.3% BSA.
- Conjugated nanobeads: Protein-coated magnetic beads.
- Storage & Handling
- All components should be stored undiluted between 2°C and 8°C.
Cell Separation (MojoSort™) - Quality tested
- Recommended Usage
5 µl of Human TruStain FcX™ (Fc Receptor Blocking Solution) for 1 x 107 cells in 100 µl of buffer.
10 µl of Human CD14 Nanobeads for 1 x 107 cells in 100 µl of buffer.
- Application Notes
This kit is designed for the positive selection or depletion of human CD14+ cells from peripheral blood mononuclear cells (PBMCs).
Antibody or cocktail dilution to use in column: N/A
Nanobead dilution to use in columns: 10X
To find out the dilution to use this product in separation columns please download the CoA.
- Is there a way to detach your magnetic particles from the cell surface?
No, not currently. We have found that cells are functional without the need to detach the magnetic Nanobeads.
- What is the size of your magnetic particles?
The average diameter is approximately 130 nm.
- Are MojoSort™ Nanobeads compatible with other commercially available magnetic separation systems?
MojoSort™ magnetic particles can be used with other commercially available magnetic separators, both free standing magnets and column-based systems. Because MojoSort™ protocols are optimized for the MojoSort™ separator, the protocols may need to be adjusted for other systems. Please contact BioLegend Technical Service for more information and guidance. We do not recommend using MojoSort™ particles for BD’s IMag™ or Life Technologies’ DynaMag™.
- What antibodies are present in the depletion cocktails provided for isolation kits?
Please contact our technical service team for further assistance.