- Other Names
- Human Cytokine Panel 2 Mix and Match Subpanel, Multiplex assay, Flow cytometry, Inflammation, Flex set, Cytokines
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- Product Citations
The LEGENDplex™ Human Cytokine Panel 2 Standard product is intended for use with the Mix and Match Human Cytokine Panel 2 of products. The compatible corresponding reagents are as follows:
740116 (LEGENDplex™ Human Cytokine Panel 2 Detection Antibodies)
740075 (LEGENDplex™ Buffer Set)
740103 (LEGENDplex™ Human TSLP Capture Bead A4, 13X)
740104 (LEGENDplex™ Human IL-1α Capture Bead A5, 13X)
740105 (LEGENDplex™ Human IL-1β Capture Bead A6, 13X)
740106 (LEGENDplex™ Human GM-CSF Capture Bead A7, 13X)
740107 (LEGENDplex™ Human IFN-α2 Capture Bead A8, 13X)
740108 (LEGENDplex™ Human IL-23 Capture Bead A10, 13X)
740109 (LEGENDplex™ Human IL-12 (p40) Capture Bead B2, 13X)
740110 (LEGENDplex™ Human IL-12 (p70) Capture Bead B3, 13X)
740111 (LEGENDplex™ Human IL-15 Capture Bead B4, 13X)
740112 (LEGENDplex™ Human IL-18 Capture Bead B5, 13X)
740113 (LEGENDplex™ Human IL-11 Capture Bead B6, 13X)
740114 (LEGENDplex™ Human IL-27 Capture Bead B7, 13X)
740115 (LEGENDplex™ Human IL-33 Capture Bead B9, 13X)
Learn more about LEGENDplex™ at biolegend.com/legendplex
Download the LEGENDplex™ software here.
- Application Notes
Note: The target analyte IFN-α2 was previously listed as IFN-α. We have since determined that the antibody reagents used in this product are specific to only IFN-α2, and not all IFN-α isoforms.
- Biology Area
- Cell Biology, Neuroinflammation, Neuroscience, Innate Immunity, Immunology
- Molecular Family
- Gene ID
- After I finish the staining process, how long can I wait before reading my LEGENDplex™ samples?
The samples can be kept overnight at 4°C while being protected from exposure to light and be read the next day. There may be a decrease in signal, but overall, the assay results should not be affected. Storing the samples for extended periods of time is not recommended, as it could lead to further reductions in signal.
- If I don't have a vacuum, how do I remove the liquid from my plate?
Do NOT invert the plate to dump the liquid. The beads will not adhere to the plate. After centrifugation using a swing bucket rotor with a plate adaptor, you can remove the liquid by using a pipette.
- Is special software required for data analysis?
Typically flow cytometers generate output files in FCS format (e.g. FCS 2.0, 3.0, or 3.1) and in some cases in list mode file format (LMD). FCS files can be analyzed by different software. Data generated from using LEGENDplex™ kits can be analyzed using the LEGENDplex™ data analysis software freely available on the BioLegend website (biolegend.com/legendplex/software). A free of charge software license dongle is currently provided in the kit. Please check our website for the most updated versions of the software.
- Should I perform the assay with the filter plates or with V-bottom plates?
Filter plates or V-bottom plates have been included in some kits for your convenience. Performing the assay with filter plates is generally recommended. A vacuum filtration unit is required to work with the filter plates. If however, you don’t have access to a vacuum manifold, then you can use the V-bottom plates or micro-FACS tubes as described in the manual and follow the recommended assay protocols for the type of plates or tubes you choose. All tubes and plates should be made from low binding polypropylene. Polystyrene ELISA or cell culture plates should not be used.
- What is the shelf life of LEGENDplex™ kits?
LEGENDplex™ kits are valid for 6 months from the date of receipt.