LEGEND MAX™ Mouse OVA Specific IgE ELISA Kit

Pricing & Availability
Regulatory Status
RUO
Other Names
OVA-IgE Precoated ELISA Kit
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Product Citations
publications
OVA-IGE_Mouse_LegMax_ELISA_083112
  • OVA-IGE_Mouse_LegMax_ELISA_083112
Cat # Size Price Quantity Check Availability Save
439807 1 Pre-coated Plate 632,00€
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Description

Immunoglobulin E has long been regarded as a major molecular component of atopic diseases, including allergy and asthma. Asthma is a chronic respiratory disease characterized by bronchial inflammation and airway hyperresponsiveness. Clinical studies have found a close association between asthma and serum IgE levels. Allergen-specific IgE has been detected in nasal mucosa from allergic patient. Examination of murine models of human allergic asthma has provided novel insight into the pathobiologic events linking airway inflammation with airway dysfunction.

Ovalbumin (OVA) is the main protein found in egg white, making up 60-65% of the total protein. Ovalbumin is an important protein in several different areas of research and commonly used to stimulate an allergic reaction in mice (e.g. established model allergen for airway hyperresponsiveness).

The BioLegend LEGEND MAX™ Mouse OVA specific IgE ELISA Kit is a Sandwich Enzyme-Linked Immunosorbent Assay (ELISA) with a 96-well strip plate that is pre-coated with OVA. This kit is specifically designed for the accurate quantitation of mouse OVA specific IgE from cell culture supernatant, serum, plasma, and other biological fluids. It is analytically validated with ready-to-use reagents.

Product Details
Technical Data Sheet (pdf)

Kit Contents

Kit Contents
  • OVA Pre-coated 96-well Strip Microplate
  • Mouse IgE Dectection Antibody
  • Mouse OVA specific IgE Standard
  • Matrix A (for serum and plasma samples only)
  • Avidin-HRP D
  • Assay Buffer A
  • Wash Buffer (20X)
  • Substrate Solution F
  • Stop Solution
  • Plate Sealers

Product Details

Reactivity
Mouse
Application

ELISA

Application References

(PubMed link indicates BioLegend citation)
  1. Miller M, et al. 2014. J. Immunol. 192:3475. PubMed
  2. Nau F, et al. 2015. Am J Physiol Lung Cell Mol Physiol. 308:191. PubMed
Product Citations
  1. Le H, et al. 2017. Biochem Biophys Res Commun. 10.1016/j.bbrc.2017.06.038. PubMed
  2. Lee H, et al. 2017. Exp Lung Res. 10.1080/01902148.2017.1339141. PubMed
  3. Seo YS, et al. 2019. Sci Rep. 9:2312. PubMed
  4. Ding FX, et al. 2018. Pediatr Res. 84:125. PubMed
  5. Xu H, et al. 2019. Exp Ther Med. 1.278472222. PubMed
  6. Miller M, et al. 2014. J Immunol. 192:3475. PubMed
  7. Nau F, et al. 2015. Am J Physiol Lung Cell Mol Physiol. 308:191. PubMed
  8. Ren J, et al. 2015. PLoS One. 10: e0140018. PubMed
  9. Fakih D, et al. 2015. Am J Physiol Lung Cell Mol Physiol. 309: L1333 - L1343. PubMed
  10. Cho S, et al. 2016. J Exp Med. 213: 235 - 249. PubMed
  11. Li C, et al. 2016. PLoS One. 11: 0161164. PubMed
Sensitivity
20.7 pg/mL
Standard Range
0.156-10 ng/mL
Materials Not Included
  • Microplate reader able to measure absorbance at 450 nm
  • Adjustable pipettes to measure volumes ranging from 1 µL to 1,000 µL
  • Deionized water
  • Wash bottle or automated microplate washer
  • Log-Log graph paper or software for data analysis
  • Tubes to prepare standard dilutions
  • Timer
  • Plate Shaker
  • Polypropylene vials

Related FAQs

In your LEGEND MAX™ ELISA Kits, there is a step that calls for washing the plates before adding sample. What is the purpose of this step?

We typically use a stabilizer for pre-coated plates. The additional washing step is designed to remove these components before you start the assay. If you do not perform the washing, the effect on assay performance is negligible.

I have multiple LEGEND MAX™ ELISA kits that I want to run simultaneously. Can I use the same wash buffer for all the kits?

The wash buffer provided in all our LEGEND MAX™ kits is the same and the part numbers on the wash buffer bottles in these kits should identical. For ELISA MAX™ Deluxe and ELISA MAX™ Standard Sets, we provide a recipe for the wash buffer on each kit’s technical data sheet. This recipe is the same for all ELISA MAX™ sets.

For some of your ELISA kits, why do my serum samples require dilution with assay buffer?

In some cases, dilution with assay buffer is required to minimize the matrix difference between the samples and the standards to achieve better accuracy.

Go To Top Version: 1    Revision Date: 11/30/2012

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

BioLegend Inc., 8999 BioLegend Way, San Diego, CA 92121 www.biolegend.com
Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

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