Pricing & Availability
Regulatory Status
Other Names
IL-8 Pre-coated ELISA Kit
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Product Citations
human il-8_121609
  • human il-8_121609
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431507 1 Pre-coated Plate 656€
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Human IL-8 (CXCL8, neutrophil chemotactic factor, neutrophil activating protein, monocytes derived neutrophil chemotactic factor) is a member of the alpha (C-X-C) subfamily of chemokines. In response to proinflammatory stimuli, IL-8 is produced by monocytes, macrophages, T cells, neutrophils, and fibroblasts. IL-8 promotes neutrophil chemotaxis and degranulation leading to local inflammation in damaged or infected tissues.

BioLegend’s LEGEND MAX™ Human IL-8 ELISA Kit is a sandwich Enzyme-Linked Immunosorbent Assay (ELISA), in which, a Human IL-8 specific monoclonal antibody is pre-coated on a 96-well strip-well plate.

BioLegend’s LEGEND MAX™ Human IL-8 ELISA Kit is specifically designed for the accurate quantitation of Human IL-8 from cell culture supernatant, serum, plasma or other bodily fluids. It is ready-to-use, accurate, and sensitive.

Product Details
Technical Data Sheet (pdf)

Kit Contents

Kit Contents
  • Anti-Human IL-8 Pre-coated 96-well Strip Microplate
  • Human IL-8 Dectection Antibody
  • Human IL-8 Standard
  • Matrix A (for serum and plasma samples only)
  • Avidin-HRP A
  • Assay Buffer A
  • Wash Buffer (20X)
  • Substrate Solution F
  • Stop Solution
  • Plate Sealers

Product Details

Verified Reactivity


Application References

(PubMed link indicates BioLegend citation)
  1. Shi JX, et al. 2012. Am J Physiol Cell Mol Physiol. 302:793. PubMed
  2. Kummaraputugu AB, et al. 2013. J. Biol Chem. 288:4681. PubMed
  3. Shi JX, et al. 2014. Cell Signal. 26:2390. PubMed
Product Citations
  1. Zhou J, et al. 2021. Front Immunol. 12:656242. PubMed
  2. Shi J, et al. 2014. Int Immunopharmacol. 22:480. PubMed
  3. Zhang Y, et al. 2018. Cell Mol Gastroenterol Hepatol. 5:611. PubMed
  4. Shi J, et al. 2014. Cell Signal. 26:2390. PubMed
  5. Spittaels KJ, et al. 2021. iScience. 24:102575. PubMed
  6. Bigenwald C, et al. 2021. Nat Med. 27:851. PubMed
  7. Shi J, et al. 2012. Am J Physiol Lung Cell Mol Physiol. 302:L793. PubMed
  8. Shi JX, et al. 2018. Mol Med Rep. 2.361805556. PubMed
  9. Dudau M, et al. 2021. Front Pharmacol. 12:737571. PubMed
  10. Kummarapurugu A, et al. 2013. J Biol Chem. 288:4861. PubMed
4.4 pg/mL
Standard Range
15.6-1,000 pg/mL
Materials Not Included
  • Microplate reader able to measure absorbance at 450 nm
  • Adjustable pipettes to measure volumes ranging from 1 µL to 1,000 µL
  • Deionized water
  • Wash bottle or automated microplate washer
  • Log-Log graph paper or software for data analysis
  • Tubes to prepare standard dilutions
  • Timer
  • Plate Shaker
  • Polypropylene vials

Antigen Details

Cell Sources
Monocytes, lymphocytes, granulocytes, fibroblasts, endothelial cells, bronchial epithelial cells, keratinocytes, melanocytes, hepatocytes, mesangial cells, chondrocytes
Biology Area
Cell Biology, Immunology, Neuroinflammation, Neuroscience
Molecular Family
Gene ID
3576 View all products for this Gene ID
View information about IL-8 on UniProt.org

Related FAQs

In your LEGEND MAX™ ELISA Kits, there is a step that calls for washing the plates before adding sample. What is the purpose of this step?

We typically use a stabilizer for pre-coated plates. The additional washing step is designed to remove these components before you start the assay. If you do not perform the washing, the effect on assay performance is negligible.

I have multiple LEGEND MAX™ ELISA kits that I want to run simultaneously. Can I use the same wash buffer for all the kits?

The wash buffer provided in all our LEGEND MAX™ kits is the same and the part numbers on the wash buffer bottles in these kits should be identical. For ELISA MAX™ Deluxe and ELISA MAX™ Standard Sets, we provide a recipe for the wash buffer on each kit’s technical data sheet. This recipe is the same for all ELISA MAX™ sets.

For some of your ELISA kits, why do my serum samples require dilution with assay buffer?

In some cases, dilution with assay buffer is required to minimize the matrix difference between the samples and the standards to achieve better accuracy.

Go To Top Version: 2    Revision Date: 09/11/2013

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.


*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.


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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587


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