LEGEND MAX™ Human IL-29 (IFN-λ1) ELISA Kit

Pricing & Availability
Other Names
IL-29, IL29, Interleukin-29, Interleukin 29, IFN-λ1, IFNL1, interferon lambda 1
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HU_IL29_IFNl1_LEGENDMAX_121917
  • HU_IL29_IFNl1_LEGENDMAX_121917
Cat # Size Price Quantity Save
446307 1 Pre-coated Plate 632€
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Description

Interleukin-29 (IL-29, IFN-λ1) is a member of the type III interferon family of cytokines. It shares a high degree (~80%) of amino acid sequence homology with both IL-28A (IFN-λ2) and IL-28B (IFN-λ3). IL-29 is produced by human peripheral blood mononuclear and dendritic cells in response to viral infection, TLR stimulation, or other type I IFN agonists. IL-29 has been demonstrated to upregulate MHC I expression on target cells; in addition, IL-29 also mediates the antiviral immune response to several viruses including Hepatitis B (HBV), Hepatitis C (HCV), and Human Immunodeficiency Virus (HIV). Increased serum levels of IL-29 have also been reported in Hashimoto’s thyroiditis patients.

The BioLegend LEGEND MAX™ Human IL-29 (IFN-λ1) ELISA Kit is a Sandwich Enzyme-Linked Immunosorbent Assay (ELISA) with a 96-well strip plate that is pre-coated with a polyclonal goat anti-human IL-29 capture antibody. The detection antibody is a biotinylated polyclonal goat anti-human IL-29 antibody. This kit is specifically designed for the accurate quantitation of human IL-29 (IFN-λ1) from cell culture supernatant, serum, plasma, and other biological fluids. This kit is analytically validated with ready-to-use reagents.

Product Details
Technical Data Sheet (pdf)

Kit Contents

Kit Contents
  • Anti-human IL-29 Pre-coated 96-well Strip Microplate
  • Human IL-29 Dectection Antibody
  • Human IL-29 Standard
  • Avidin-HRP
  • Assay Buffer A
  • Wash Buffer (20X)
  • Substrate Solution F
  • Stop Solution
  • Plate Sealers

Product Details

Reactivity
Human
Application

ELISA

Sensitivity
2.4 pg/mL
Standard Range
7.8 - 500 pg/mL
Materials Not Included
  • Microplate reader able to measure absorbance at 450 nm
  • Adjustable pipettes to measure volumes ranging from 1 µL to 1,000 µL
  • Deionized water
  • Wash bottle or automated microplate washer
  • Log-Log graph paper or software for data analysis
  • Tubes to prepare standard dilutions
  • Timer
  • Plate Shaker
  • Polypropylene vials

Antigen Details

Biology Area
Immunology
Molecular Family
Cytokines/Chemokines, Growth Factors
Gene ID
282618 View all products for this Gene ID
UniProt
View information about IL-29 on UniProt.org

Related FAQs

For some of your ELISA kits, why do my serum samples require dilution with assay buffer?

Dilution with assay buffer is required to minimize the matrix difference between the samples and the standards to achieve better accuracy.

I have multiple LEGEND MAX™ ELISA kits that I want to run simultaneously. Can I use the same wash buffer for all the kits?

The Wash buffer is the same for all the current LEGEND MAX™ kits. All the part numbers on the Wash Buffer bottles in these kits should be the same. For ELISA MAX™ Deluxe and ELISA MAX™ Standard sets, we provide a recipe for the wash buffer on each kit’s technical data sheet. This recipe is the same for all ELISA MAX™ sets.

In your LEGEND MAX™ ELISA Kits, there is a step that calls for a washing of the plates before even adding any sample to it. What is the purpose of this step?

We typically use a stabilizer for pre-coated plates. The washings were designed to remove these components before you start the assay. If you do not do the washings, the effect on assay performance is negligible.

Go To Top Version: 1    Revision Date: 12/21/2017

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

BioLegend Inc., 8999 BioLegend Way, San Diego, CA 92121 www.biolegend.com
Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

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