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This antibody is effective in immunohistochemistry (IHC).Product Details
- Product Information
In Vitro Diagnostic (IVD). Use in immunohistochemistry (IHC) test methods only.
The polyclonal antibody Poly18339 is used for the in vitro examination of human biological samples using immunohistochemistry (IHC) methods for the qualitative identification of Keratin 15. The clinical interpretation of any staining or its absence should be complemented by morphological studies and proper controls and should be evaluated within the context of the patient's clinical history and other diagnostic tests by a qualified pathologist.
- This monospecific polyclonal antibody was raised in chickens against a peptide sequence derived from the C-terminus of the human keratin 15 protein, CDGQVVSSHKREI.
- Phosphate-buffered solution + 0.03% Thimerosal.
- The antibody was purified by IgY affinity chromatography.
- 1.0 mg/mL
- Storage & Handling
- When stored at ≤ -20°C, product is stable until the date shown on the label. Avoid repeated freeze-thaw cycles to prevent denaturing the antibody. If thawed and stored at 2-8°C, product is stable for 24 months from the date of thaw or until the expiry date on the label, whichever comes first.
- Recommended Usage
Each lot of this antibody is quality control tested by immunohistochemical staining.
The optimal working dilution should be determined for each specific assay condition.
• IHC: 1:500 with a biotin based detection system such as biotinylated anti-chicken immunoglobulin and USA Ultra Streptavidin Detection (Cat. No. 931001).
Tissue Sections: Paraffin-embedded tissues
Pretreatment: For optimal staining, the sections should be pretreated with an antigen unmasking solution such as Citrate Buffer Retrieval solution (Cat. No. 928502).
Incubation: 60 minutes at room temperature
(PubMed link indicates BioLegend citation)
- Liu Y, Lyle S, Yang Z, Cotsarelis G. Keratin 15 promoter targets putative epithelial stem cells in the hair follicle bulge. J Invest Dermatol 121(5):963-8, 2003. [IHC]
- Jih DM, Lyle S, Elenitsas R, Elder DE, Cotsarelis G. Cytokeratin 15 expression in trichoepitheliomas and a subset of basal cell carcinomas suggests they originate from hair follicle stem cells. J Cutan Pathol 26(3):113-118, 1999.
- Leube RE, Bader BL, Bosch FX, Zimbelmann R, Achtstaetter T, Franke WW. Molecular characterization and expression of the stratification-related cytokeratins 4 and 15. J Cell Biol 106(4):1249-1261, 1988.
- Product Citations
AB_2564970 (BioLegend Cat. No. 833901)
WARNINGS AND PRECAUTIONS
- Use appropriate personal protective equipment and safety practices per universal precautions when working with this reagent. Refer to the reagent safety data sheet.
- All specimens, samples and any material coming in contact with them should be considered potentially infectious and should be disposed of with proper precautions and in accordance with federal, state and local regulations.
- Do not use this reagent beyond the expiration date stated on the label.
- Do not use this reagent if it appears cloudy or if there is any change in the appearance of the reagent as these may be indication of possible deterioration.
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