- Poly4053 (See other available formats)
- Regulatory Status
- Goat Polyclonal IgG
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- Product Citations
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This polyclonal goat anti-mouse IgG antibody reacts with the heavy chains of mouse IgG and with the light (kappa and lambda) chains common to most mouse immunoglobulins. No cross-reactivity has been detected against non-immunoglobulin serum proteins. This antibody has been solid-phase absorbed to ensure minimal cross-reaction with rat, human, bovine, horse, and rabbit immunoglobulins, but it may have minimal cross-reactivity with other subclasses of mouse immunoglobulins.Product Details
- Antibody Type
- Host Species
- Aqueous buffered solution containing a carrier protein and preservatives.
- The polyclonal antibody was purified from goat antiserum by mouse immunoglobulin affinity chromatography.
- Lot-specific (please contact technical support for concentration and total µg amount, or use our Lookup tool if you have a lot number.)
- Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
This product has a shelf-life of 12 months or less. Please use our Expiration Lookup Tool to verify the expiration date of your lot of product
ELISA - Quality tested
WB, IHC - Reported in the literature, not verified in house
- Recommended Usage
Each lot of this antibody is quality control tested by ELISA assay. Do not use this product beyond the expiration date. For ELISA or Western blot analysis, the reagent should be titrated between 1:2,000 - 1:5,000 to determine optimal conditions. For IHC on tissue sections, the reagent should be titrated between 1:500 - 1:2000 to determine optimal conditions. It is recommended that the reagent be titrated for optimal performance for each application. Avoid using diluent solutions containg sodium azide. Sodium azide is an inhibitor of horseradish peroxidase.
- Application Notes
This polyclonal goat anti-mouse IgG antibody is useful for capture or detection of mouse IgG in ELISA.
(PubMed link indicates BioLegend citation)
- Scotta C, et al. 2008. J Immunol. 181:1025-33. PubMed
- Gao X, et al. 2014. Cell Mol Neurobiol. 34:257-68 (ICC)
- Winters T, et al. 2014. EMBO J. 33:1256-70.
- Product Citations
AB_315009 (BioLegend Cat. No. 405306)
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