HRP Goat anti-mouse IgG (minimal x-reactivity) Antibody

Pricing & Availability
Clone
Poly4053 (See other available formats)
Regulatory Status
RUO
Isotype
Goat Polyclonal IgG
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Product Citations
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Cat # Size Price Quantity Avail. Save
405306 500 µL 192,00€
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Description

This polyclonal goat anti-mouse IgG antibody reacts with the heavy chains of mouse IgG and with the light (kappa and lambda) chains common to most mouse immunoglobulins. No cross-reactivity has been detected against non-immunoglobulin serum proteins. This antibody has been solid-phase absorbed to ensure minimal cross-reaction with rat, human, bovine, horse, and rabbit immunoglobulins, but it may have minimal cross-reactivity with other subclasses of mouse immunoglobulins.

Product Details
Technical Data Sheet (pdf)

Product Details

Reactivity
Mouse
Antibody Type
Polyclonal
Host Species
Goat
Formulation
Aqueous buffered solution containing a carrier protein and preservatives.
Preparation
The polyclonal antibody was purified from goat antiserum by mouse immunoglobulin affinity chromatography.
Concentration
Lot-specific (please contact technical support for concentration and total µg amount, or use our Lookup tool if you have a lot number.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.

This product has a shelf-life of 12 months or less. Please use our Expiration Lookup Tool to verify the expiration date of your lot of product
Application

ELISA - Quality tested
WB, IHC - Reported in the literature, not verified in house

Recommended Usage

Each lot of this antibody is quality control tested by ELISA assay. Do not use this product beyond the expiration date. For ELISA or Western blot analysis, the reagent should be titrated between 1:2,000 - 1:5,000 to determine optimal conditions. For IHC on tissue sections, the reagent should be titrated between 1:500 - 1:2000 to determine optimal conditions. It is recommended that the reagent be titrated for optimal performance for each application. Avoid using diluent solutions containg sodium azide. Sodium azide is an inhibitor of horseradish peroxidase.

Application Notes

This polyclonal goat anti-mouse IgG antibody is useful for capture or detection of mouse IgG in ELISA.

Application References

(PubMed link indicates BioLegend citation)
  1. Scotta C, et al. 2008. J Immunol. 181:1025-33. PubMed
  2. Gao X, et al. 2014. Cell Mol Neurobiol. 34:257-68 (ICC)
  3. Winters T, et al. 2014. EMBO J. 33:1256-70.
Product Citations
  1. Ortega-Prieto AM, et al. 2018. Nat Commun. 9:682. PubMed
  2. Miyamoto M, et al. 2018. Oncol Lett. 15:4005. PubMed
  3. Chen G, et al. 2018. Front Immunol. 9:549. PubMed
  4. Omer S et al. 2018. eLife. 7 pii: e36745. PubMed
  5. Wang C, et al. 2019. PLoS Pathog. 15:e1007938. PubMed
  6. Chung KP, et al. 2019. Nat Commun. 10:3390. PubMed
  7. Chiang CF, et al. 2019. Sci Rep. 9:12448. PubMed
  8. Martinov T, et al. 2019. J Immunol. 203:844. PubMed
  9. Ventura E, et al. 2018. J Cell Sci. 131. PubMed
  10. Biering SB et al. 2017. Cell host & microbe. 22(1):74-85 . PubMed
  11. Prakash P, et al. 2019. Methods Protoc. 2:48. PubMed
  12. Zhang B, et al. 2020. J Exp Med. 217:e20182365. PubMed
  13. Jin SW, et al. 2019. Cell Rep. 29:1449. PubMed
  14. Parks KR, et al. 2019. Cell Rep. 29:3060. PubMed
  15. Hoffmann D, et al. 2020. Int J Tryptophan Res. 12:1178646919891736. PubMed
  16. Schadt L, et al. 2020. Cell Reports. 29(5):1236-1248.e7.. PubMed
  17. Wu YH, et al. 2020. Mol Med Rep. 21:851. PubMed
  18. Nanna AR, et al. 2020. Nucleic Acids Res. 48:5281. PubMed
  19. Liu H, et al. 2020. Cell Death Dis. 0.767361111. PubMed
  20. Frank N, et al. 2011. Cancer Res. 71:1474. PubMed
  21. Hwang S, et al. 2012. Proc Natl Acad Sci U S A. 110:276. PubMed
  22. Horton N, et al. 2013. PLoS One. 8:e59552. PubMed
  23. Hsing C, et al. 2008. Cytokine. 44:221. PubMed
  24. Yan L, et al. 2014. Clin Vaccine Immunol. 21:1206. PubMed
  25. Copp J, et al. 2014. Proc Natl Acad Sci U S A. 111:13481. PubMed
  26. Collin R, et al. 2014. J Immunol. 193:3503. PubMed
  27. Shkoporov A, et al. 2015. FEMS Microbiol Lett. 362:83. PubMed
  28. Nishigami Y, et al. 2016. Sci Rep. 6:18964. PubMed
  29. Sertorio M, et al. 2016. J Immunol. 196: 2986-2994. PubMed
  30. Spanier J, et al. 2016. Nat Commun. 7:11804. PubMed
  31. Vrieling M, et al. 2016. Sci Rep. 6:37759. PubMed
  32. Hoffmann D, et al. 2020. Cancer Immunol Res. 8:19. PubMed
  33. Ram AK, et al. 2020. World J Gastroenterol. 26:7131. PubMed
  34. Lee K, et al. 2020. Biology (Basel). 9:00. PubMed
  35. Israelow B, et al. 2020. J Exp Med. 217:00:00. PubMed
  36. Hsu HW, et al. 2020. Development. 147:00:00. PubMed
  37. Furlong K, et al. 2020. J Virol. 94:00:00. PubMed
  38. Boyt DT, et al. 2020. J R Soc Interface. 17:20190815. PubMed
  39. Christin JR, et al. 2020. Cell Reports. 31(10):107742. PubMed
  40. Uka R, et al. 2020. Oncogene. 39:4132. PubMed
  41. Gupta A, et al. 2020. J Biol Chem. 295:2057. PubMed
  42. Song E, et al. 2021. J Exp Med. 218:00:00. PubMed
  43. Yaguchi J, et al. 2021. BMC Biology. 19(1):64. PubMed
  44. Lin YR, et al. 2020. Immunity. 53(4):840-851.e6. PubMed
  45. Seydoux E, et al. 2021. Cell Reports. 35(5):109084. PubMed
  46. de Mingo Pulido , et al. 2021. Immunity. 54(6):1154-1167.e7. PubMed
RRID
AB_315009 (BioLegend Cat. No. 405306)

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For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

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