HRP anti-Gemin 3 Antibody

Pricing & Availability
Clone
12H12 (See other available formats)
Regulatory Status
RUO
Other Names
Gemin3
Isotype
Mouse IgG1, κ
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Product Citations
publications
12H12_HRP_Gemin3_Antibody_120919
Western blot of HRP anti-Gemin 3 (clone 12H12). Lane 1: Molecular weight marker; Lane 2: 20 µg of HeLa cell line lysate. The blot was incubated with 1 µg/mL of the primary antibody overnight at 4°C. Enhanced chemiluminescence (Cat. No. 426302) was used as the detection system.
  • 12H12_HRP_Gemin3_Antibody_120919
    Western blot of HRP anti-Gemin 3 (clone 12H12). Lane 1: Molecular weight marker; Lane 2: 20 µg of HeLa cell line lysate. The blot was incubated with 1 µg/mL of the primary antibody overnight at 4°C. Enhanced chemiluminescence (Cat. No. 426302) was used as the detection system.
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865503 25 µg 192,00€
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865504 100 µg 424,00€
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Description

Gemin 3, also known as DEAD-box helicase 20 (DDX20), is a putative RNA helicase that contains a conserved ASP-GLU-ALA-ASP (DEAD) motif. Gemin 3 is involved in a variety of cellular processes including initiation of protein translation, ribosome assembly, and alteration of RNA secondary structure. Gemin 3 is one of the core components of the survival of motor neurons (SMN) macromolecule protein complex, which plays an important role in the assembly and re-structuring of small nucleoproteins and transcriptosomes. Gemin 3 may also be involved in cancer as it acts as a tumor suppressor in hepatocellular carcinoma, and as a tumor promoter in breast cancer and prostate cancer2.

Product Details
Technical Data Sheet (pdf)

Product Details

Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Formulation
This antibody is provided in 50% glycerol in aqueous buffered solutions with preservatives.
Preparation
The antibody was purified by affinity chromatography and conjugated with HRP under optimal conditions.
Concentration
0.5 mg/mL
Storage & Handling
Upon receipt, the antibody solution should be stored undiluted at -20°C, and protected from prolonged exposure to light.
Application

WB - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by western blotting. For western blotting, the suggested use of this reagent is 0.2 - 1.0 µg/mL. It is recommended that the reagent be titrated for optimal performance for each application.

RRID
AB_2820162 (BioLegend Cat. No. 865503)
AB_2820163 (BioLegend Cat. No. 865504)

Antigen Details

Structure
Gemin 3 is an 824 amino acid protein with an apparent molecular mass of ~ 92 kD.
Distribution

Tissue Distribution: Testis and ovary
Cellular Distribution: Cytosol, nucleus, and cytoskeleton

Function
Gemin 3 is a component of SMN macromolecule protein complex.
Interaction
SMN, GEMIN4
Cell Type
Astrocytes, GABAergic Neurons, Glutamatergic Neurons, Mature Neurons, Microglia, Neural Stem Cells, Neurons, Oligodendrocytes
Biology Area
Cancer Biomarkers, Neurodegeneration, Neuroscience, Synaptic Biology, Transcription Factors
Antigen References
  1. Seng CO, et al. 2015. Hum. Mol. Genet. 24(8):2138.
  2. Chen W, et al. 2016. Int. J. Mol. Med. 37(6):1551.
Gene ID
11218 View all products for this Gene ID
UniProt
View information about Gemin 3 on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 1    Revision Date: 12/09/2019

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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