Go-ChIP-Grade™ Purified anti-STAT5 Phospho (Tyr694) Antibody

Pricing & Availability
Clone
A17016B (See other available formats)
Other Names
Signal transducer and activator of transcription 5A
Isotype
Mouse IgG1, κ
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Product Citations
publications
1_A17016B_ChIP_STAT5-Phospho_Antibody_1_042718
Chromatin Immunoprecipitations (ChIP) were performed with cross-linked chromatin samples from 4 x 106 of Ba/F3 cells deprived for IL-3 overnight and then treated with IL-3 with either A)1:50 dilution of Go-ChIP-Grade™ Purified anti-STAT5 Phospho (Tyr694) (Clone A17016B) or B) equal amount of Purified Mouse IgG1, κ Isotype Control Antibody (Clone MG1-45, Cat. No. 401401) by using Go-ChIP-Grade™ Protein G Enzymatic Kit (Cat. No. 699904). The enriched DNA was purified and quantified by real-time qPCR using primers targeting mouse SOCS3 gene region and α-Satellite repeats. The amount of immunoprecipitated DNA in each sample is represented as signal relative to total amount of input chromatin.
  • 1_A17016B_ChIP_STAT5-Phospho_Antibody_1_042718
    Chromatin Immunoprecipitations (ChIP) were performed with cross-linked chromatin samples from 4 x 106 of Ba/F3 cells deprived for IL-3 overnight and then treated with IL-3 with either A)1:50 dilution of Go-ChIP-Grade™ Purified anti-STAT5 Phospho (Tyr694) (Clone A17016B) or B) equal amount of Purified Mouse IgG1, κ Isotype Control Antibody (Clone MG1-45, Cat. No. 401401) by using Go-ChIP-Grade™ Protein G Enzymatic Kit (Cat. No. 699904). The enriched DNA was purified and quantified by real-time qPCR using primers targeting mouse SOCS3 gene region and α-Satellite repeats. The amount of immunoprecipitated DNA in each sample is represented as signal relative to total amount of input chromatin.
  • 2_A17016B_GoChIP-PURE_STAT5_Antibody_FC_091218
    Human peripheral blood mononuclear cells were treated with (filled histogram), or without (open histogram) Recombinant Human IL-2 (carrier free, Cat. No. 589102) for 15 minutes, fixed with Fixation Buffer (Cat. No. 420801), permeabilized with True-Phos™ Perm Buffer (Cat. No. 425401) then stained with Go-ChIP-Grade™ Purified anti-STAT5 Phospho (Tyr694) (clone A17016B), and followed by mouse IgG PE. Data was gated on the lymphocyte population.
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699603 25 µg 152€
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699604 100 µg 360€
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This product is scheduled for discontinuation. Please use the availability checker to determine current availability.
Description

STAT5 is a member of the signal transducer and activator of transcription factors (STAT) family, mediating growth and cytokine signaling. STAT5 consists of two closely related family members, STAT5A and STAT5B, which exhibit 96% sequence homology and are functionally redundant. Upon activation, STAT5 is phosphorylated by receptor tyrosine kinases and, in turn, forms homodimers or hererodimers with other family members through its SH2 domains. The dimerized STAT5 translocates to the nucleus and binds to the STAT5 response element (TTCXXXGAA). STAT5 plays crucial regulatory roles in cell proliferation, anti-apoptosis, early B cell differentiation, and T cell development.

Product Details
Technical Data Sheet (pdf)

Product Details

Reactivity
Human, Mouse†
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Phosphorylated human STAT5A at Tyr694
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

ChIP - Quality tested
ICFC - Validated

Recommended Usage

Each lot of this antibody is quality control tested by ChIP Assay. The suggested dilution for ChIP application is 1:50 - 1:200 by volume. For flow cytometric staining, the suggested use of this reagent is ≤0.125 µg per million cells in 100 µl volume. It is recommended that the reagent to be titrated for optimal performance before each experiment.

Application Notes

Does not cross-react with mouse for ICFC.

†Based on in-house testing, clone A17016B cross-reacts to Mouse STAT5 Phospho (Tyr694) in Western blots, but it is not recommended for intracellular flow cytometry application due to dim staining

RRID
AB_2734545 (BioLegend Cat. No. 699603)
AB_2734546 (BioLegend Cat. No. 699604)

Antigen Details

Structure
794 amino acids, predicted molecular weight of 91 kD; contains a SH2 domains responsible for homodimerization or heterodimerization.
Distribution

Cytoplasm; phosphorylated form translocates to the nucleus.

Function
STAT5 is a transcription regulator and is involved in cell differentiation, proliferation, and survival, in response to several cytokines, such as interleukins, erythropoietin, growth hormone, thrombopoietin, and prolactin.
Interaction
Forms a homodimer or a heterodimer with other family members. Interacts with NCOA1, SOCS7, CBP, p300, SMRT and ERBB4.
Cell Type
B cells, Th17, Tregs
Biology Area
Apoptosis/Tumor Suppressors/Cell Death, Cell Biology, Immunology, Signal Transduction, Transcription Factors
Molecular Family
Nuclear Markers, Phospho-Proteins
Antigen References

1. Bell BD, et al. 2013. Nat. Immunol. 14:364.
2. Cholez E, et al. 2012. Leukemia. 26:2390.
3. Heltemes-Harris LM, et al. 2012. Curr. Opin. Immunol. 24:146.
4. Heltemes-Harris LM, et al. 2011. Ann. N Y Acad. Sci. 1217:18.
5. Malin S, et al. 2010. Curr. Opin. Immunol. 22:168.
6. Xu J, et al. 2009. J. Neurosci. 29:2022.

Gene ID
6776 View all products for this Gene ID
UniProt
View information about STAT5 Phospho on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 1    Revision Date: 04/30/2018

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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