Go-ChIP-Grade™ Purified anti-RUNX3 Antibody

Pricing & Availability
Clone
9F4A17 (See other available formats)
Other Names
Runt-related transcription factor 3, Polyomavirus enhancer-binding protein 2 alpha C subunit (PEBP2 alpha C), Acute myeloid leukemia 2 (AML2)
Isotype
Mouse IgG2a, κ
Ave. Rating
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Product Citations
publications
9F4A17_GoChIP_Runx3_Antibody_051818
Chromatin Immunoprecipitation (ChIP) was performed with cross-linked chromatin samples from 4 X 106 Jurkat cells with either A)1:50 dilution of Go-ChIP-Grade™ Purified anti-Runx3 (Clone 9F4A17, Cat. No. 653603) or B) equal amount of Purified Mouse IgG2a, κ Isotype Control Antibody (Clone MOPC-173, Cat. No. 400201) by using Go-ChIP-Grade™ Protein G Enzymatic Kit (Cat. No. 699904). The enriched DNA was purified and quantified by real-time qPCR using primers targeting human PR3 gene region or α-Satellite repeats. The amount of immunoprecipitated DNA in each sample is represented as signal relative to total amount of input chromatin.
  • 9F4A17_GoChIP_Runx3_Antibody_051818
    Chromatin Immunoprecipitation (ChIP) was performed with cross-linked chromatin samples from 4 X 106 Jurkat cells with either A)1:50 dilution of Go-ChIP-Grade™ Purified anti-Runx3 (Clone 9F4A17, Cat. No. 653603) or B) equal amount of Purified Mouse IgG2a, κ Isotype Control Antibody (Clone MOPC-173, Cat. No. 400201) by using Go-ChIP-Grade™ Protein G Enzymatic Kit (Cat. No. 699904). The enriched DNA was purified and quantified by real-time qPCR using primers targeting human PR3 gene region or α-Satellite repeats. The amount of immunoprecipitated DNA in each sample is represented as signal relative to total amount of input chromatin.
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653603 25 µg 120€
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653604 100 µg 376€
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Description

RUNX3 belongs to the runt-domain family of transcription factors that regulates gene expression in major developmental pathways, such as T-cell differentiation and neurogenesis. RUNX3 is epigenetically silenced in many types of cancers. In gastric cancer, RUNX3 has been frequently reported to be inactivated by gene silencing or protein mislocalization. The oncoprotein SRC phosphorylates RUNX3 at multiple tyrosine residues, resulting in cytoplasmic mislocalization of RUNX3. RUNX3 is regulated by the TGF-B/Smad pathway, and induces apoptosis or cell cycle arrest of gastric cancer cells through upregulating expression of p21 and Bim. The loss of RUNX3 function in T-cells cause suppression of Treg function and formation of colitis tumor. These findings suggest an important role of RUNX3 in tumor suppression.

Product Details
Technical Data Sheet (pdf)

Product Details

Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Partial human RUNX3 recombinant protein (194-304 aa).
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

ChIP - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by ChIP Assay. The suggested dilution for ChIP application is 1:50-1:100 by volume. It is recommended that the reagent to be titrated for optimal performance before each experiment.

Application Notes

The binding of RUNX3 antibody (9F4A17) to its target is sensitive to salt concentration.

RRID
AB_2687117 (BioLegend Cat. No. 653603)
AB_2687118 (BioLegend Cat. No. 653604)

Antigen Details

Structure
44 kD protein containing a runt domain and a proline/serine/threonine rich domain required for transcriptional activation.
Distribution

Nucleus, the tyrosine phosphorylated form is localized to the cytoplasm.

Function
RUNX3 is a transcription factor that forms a heterodimeric complex with CBF beta subunit. The RUNX3 heterodimer binds to numerous enhancers and promoters, activating or suppressing target genes. RUNX3 was reported to function as a tumor suppressor.
Interaction
RUNX3 interacts with TLE1, SUV39H1, SRC, FYN, and LCK.
Cell Type
Tregs
Biology Area
Apoptosis/Tumor Suppressors/Cell Death, Cell Biology, Cell Cycle/DNA Replication, Immunology, Transcription Factors
Molecular Family
Nuclear Markers
Antigen References

1. Goh YM, et al. 2010. J. Biol. Chem. 285:10122. 
2. Vogiatzi P, et al. 2006. Cancer Biol. Ther. 5:371. 
3. Inoue K, et al. 2008. Neural Dev. 3:20. 
4. Sugai M, et al. 2011. J. Immunol. 186:6515. 
5. Ito K, et al. 2005. Cancer Res. 65:7743. 
6. Hsu PI, et al. 2009. Ann. Surg. Oncol. 16:1686.

Gene ID
864 View all products for this Gene ID
UniProt
View information about RUNX3 on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 3    Revision Date: 05/18/2018

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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