Go-ChIP-Grade™ Purified anti-IRF4 Antibody

Pricing & Availability
Clone
IRF4.3E4 (See other available formats)
Other Names
Interferon regulatory factor 4
Isotype
Rat IgG1, κ
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Product Citations
publications
IRF43E4_GoChIP_IRF4_Antibody_1_020618
Chromatin Immunoprecipitation (ChIP) was performed using Go-ChIP-Grade™ Protein G Enzymatic Kit by loading 3 µg of cross-linked chromatin samples from U266 cells treated with IFNα2 with either A) 1:200 dilution of Go-ChIP-Grade™ Purified anti-IRF4 (Clone IRF4.3E4), or B) equal amount of Purified Rat IgG1, κ Isotype Control Antibody (Cat. No. 400401). The enriched DNA was purified and quantified by real-time qPCR using primers targeting human SUB1 gene region. The amount of immunoprecipitated DNA in each sample is represented as signal relative to the 5% of total amount of input chromatin.
  • IRF43E4_GoChIP_IRF4_Antibody_1_020618
    Chromatin Immunoprecipitation (ChIP) was performed using Go-ChIP-Grade™ Protein G Enzymatic Kit by loading 3 µg of cross-linked chromatin samples from U266 cells treated with IFNα2 with either A) 1:200 dilution of Go-ChIP-Grade™ Purified anti-IRF4 (Clone IRF4.3E4), or B) equal amount of Purified Rat IgG1, κ Isotype Control Antibody (Cat. No. 400401). The enriched DNA was purified and quantified by real-time qPCR using primers targeting human SUB1 gene region. The amount of immunoprecipitated DNA in each sample is represented as signal relative to the 5% of total amount of input chromatin.
  • IRF43E4_GoChIP_IRF4_Antibody_2_020618
    Total lysates (15 µg protein) from HeLa (lane 1, negative control) and Ramos (lane 2) were resolved by electrophoresis (4-12% Bis-Tris midi gel), transferred to nitrocellulose, and probed with 1:250 diluted (2 µg/mL) Purified anti-IRF4 Antibody, clone IRF4.3E4 (upper). Proteins were visualized by chemiluminescence detection using a 1:3000 diluted anti-rat-IgG secondary antibody conjugated to HRP for the anti-IRF4 Antibody or 1:5000 diluted Direct-Blot™ HRP anti-β-actin Antibody, clone 2F1-1(lower). Lane M: Molecular weight ladder.
Cat # Size Price Quantity Avail. Save
646411 25 µg 152€
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646412 100 µg 376€
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Description

The IRF family consists of at least nine members. IRF4 and IRF8 are highly homologous to each other and also redundant in function. IRF4 is critical for Th2 and Th17 development. Together with TRF8, it plays an essential role in macrophage and dendritic cell development and function. IRF4 is also reported to be essential for pre-B cell development, receptor editing, germinal center reactor and plasma cell differentiation.

Product Details
Technical Data Sheet (pdf)

Product Details

Reactivity
Mouse, Human
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
GST fusion protein containing C-terminal of murine IRF4
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

ChIP - Quality tested
WB - Validated

Recommended Usage

Each lot of this antibody is quality control tested by ChIP Assay. The suggested dilution for ChIP application is 1:50 - 1:200 by volume. For Western blotting, the suggested use of this reagent is 10.0 µg per ml. It is recommended that the reagent to be titrated for optimal performance before each experiment.

Application References

(PubMed link indicates BioLegend citation)
  1. Zheng Y, et al. 2009. Nature 458:351
  2. Yin SY, et al. 2011. Exp Cell Res. 317:2210. PubMed
RRID
AB_2728477 (BioLegend Cat. No. 646411)
AB_2728478 (BioLegend Cat. No. 646412)

Antigen Details

Structure
Molecular weight is approximately 51 kD.
Distribution

IRF4 is reported to be expressed exclusively in the immune system.

Biology Area
Cell Biology, Transcription Factors
Molecular Family
Nuclear Markers
Antigen References

1. Lu R. 2008. Trends Immunol 29:487.

Gene ID
3662 View all products for this Gene ID
UniProt
View information about IRF4 on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 1    Revision Date: 02/07/2018

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