Go-ChIP-Grade™ Purified anti-Histone H3 (C-terminus) Antibody

Pricing & Availability
Clone
1B1-B2 (See other available formats)
Other Names
Histone H3.1, histone H3/a, histone 1, H3a, H3 histone family, member A, H3/A, H3FA
Isotype
Mouse IgG3
Ave. Rating
Submit a Review
Product Citations
publications
a-1B1-B2_ChIP_Histone_H3_Antibody_040220
Chromatin Immunoprecipitations (ChIP) were done with fixed and sonicated chromatin samples from Raji cells. ChIP was performed with 5 µg of chromatin and either 1 µg of Go-ChIP-Grade™ purified anti-Histone H3 (C-terminus), or equal amount of Go-ChIP-Grade™ purified mouse IgG3, κ isotype ctrl antibody (clone MG3-35, Cat. No. 401326). The enriched DNA was purified and quantified by real-time qPCR using SYBR Green and primers for the human GAPDH exon 1 region, the human RPL30 exon 3 region and the human α-Satellite repeat element. The amount of immunoprecipitated DNA in each sample is represented as a percentage of the total amount of input chromatin, which is equivalent to 100%.
  • a-1B1-B2_ChIP_Histone_H3_Antibody_040220
    Chromatin Immunoprecipitations (ChIP) were done with fixed and sonicated chromatin samples from Raji cells. ChIP was performed with 5 µg of chromatin and either 1 µg of Go-ChIP-Grade™ purified anti-Histone H3 (C-terminus), or equal amount of Go-ChIP-Grade™ purified mouse IgG3, κ isotype ctrl antibody (clone MG3-35, Cat. No. 401326). The enriched DNA was purified and quantified by real-time qPCR using SYBR Green and primers for the human GAPDH exon 1 region, the human RPL30 exon 3 region and the human α-Satellite repeat element. The amount of immunoprecipitated DNA in each sample is represented as a percentage of the total amount of input chromatin, which is equivalent to 100%.
  • b-1B1-B2_Purified_Histone_H3_CO2_Terminus_Antibody_IHC_030415
    IHC staining of purified anti-Histone H3 (C-Terminus) antibody (clone 1B1-B2) on formalin-fixed paraffin-embedded rat brain tissue. Following antigen retrieval using Sodium Citrate H.I.E.R., the tissue was incubated with 1 µg/mL of the primary antibody overnight at 4°C. BioLegend's Ultra Streptavidin (USA) HRP kit (Multi-Species, DAB, Cat. No. 929901) was used for detection followed by hematoxylin counterstaining, according to the protocol provided. The image was captured with a 40X objective.
  • c-1B1-B2_PURE_a-HistoneH3_IHC_Antibody_2_043018
    IHC staining of purified anti-Histone H3 (C-Terminus) antibody (clone 1B1-B2) on formalin-fixed paraffin-embedded mouse brain tissue. Following antigen retrieval using Sodium Citrate H.I.E.R., the tissue was incubated with 1 µg/mL of the primary antibody overnight at 4°C. BioLegend's Ultra Streptavidin (USA) HRP kit (Multi-Species, DAB, Cat. No. 929901) was used for detection followed by hematoxylin counterstaining, according to the protocol provided. The image was captured with a 40X objective. Scale bar: 50 µm
  • d-1B1-B2_PURE_a-HistoneH3_IHC_Antibody_3_043018
    IHC staining of purified anti-Histone H3 (C-Terminus) antibody (clone 1B1-B2) on formalin-fixed paraffin-embedded rat brain tissue. Following antigen retrieval using Sodium Citrate H.I.E.R., the tissue was incubated with 1 µg/mL of the primary antibody overnight at 4°C. BioLegend's Ultra Streptavidin (USA) HRP kit (Multi-Species, DAB, Cat. No. 929901) was used for detection followed by hematoxylin counterstaining, according to the protocol provided. The image was captured with a 40X objective. Scale bar: 50 µm
  • e-1B1-B2_PURE_Histone_H3_Antibody_4_WB_102519_updated
    Western blot of purified anti-Histone H3 (C-terminus) antibody (clone 1B1-B2). Lane 1: Molecular weight marker; Lane 2: 20 µg of human brain lysate; Lane 3: 20 µg of mouse brain lysate; Lane 4: 20µg of rat brain lysate; Lane 5: 10µg of Drosophila head lysate. The blot was incubated with 0.1 µg/mL of the primary antibody overnight at 4°C, followed by incubation with HRP Goat anti-mouse IgG Antibody (Cat. No. 405306). Enhanced chemiluminescence was used as the detection system.
  • f-1B1-B2_PURE_Histone_H3_Antibody_2_WB_051519
    Western blot of purified anti-Histone H3 (C-terminus) antibody (clone 1B1-B2). Lane 1: Molecular weight marker; Lane 2: 20 µg of Drosophila head lysate; Lane 3: 20 µg of Drosophila S2 (embryonic) cell lysate. The blot was incubated with 0.1 µg/mL of the primary antibody overnight at 4°C, followed by incubation with HRP Goat anti-mouse IgG Antibody (Cat. No. 405306). Enhanced chemiluminescence was used as the detection system.
Cat # Size Price Quantity Avail. Save
819413 25 µg 120€
Check Availability


Need larger quantities of this item?
Request Bulk Quote
819414 100 µg 376€
Check Availability


Need larger quantities of this item?
Request Bulk Quote
Description

Histone proteins are classified into core histones (H2A, H2B, H3, H4) and linker histones (H1, H5). Core histones form an octamer, which contains two H2A-H2B dimers and one H3-H4 tetramer. Core histones are predominantly globular except for the unstructured N-terminal tails. Posttranslational modifications, such as acetylation, methylation, phosphorylation, ubiquitination, SUMOylation and ADP-ribosylation occur in histone tails.

Histone modifications induce changes of chromatin structure and thereby affect the accessibility of transcription factors, nuclear proteins and enzymes to genomic DNA, resulting in gene activation or repression. It is known that histone modifications play critical roles in DNA repair, DNA replication, transcription regulation, alternative splicing and chromosome condensation and some diseases including autoimmune diseases and cancers.

Product Details
Technical Data Sheet (pdf)

Product Details

Reactivity
Human, Mouse, Rat, Drosophila
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
This monoclonal antibody was raised against a peptide sequence derived from the C-terminal domain of human Histone H3 protein.
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide with stabilizer
Concentration
0.5 mg/mL
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

ChIP - Quality tested
IHC-P, WB - Validated

Recommended Usage

The recommended starting antibody to chromatin ratio is 1 µg of antibody and 5 µg of chromatin per IP. Optimal antibody to chromatin ratio should be determined by the end user. For immunohistochemistry on formalin-fixed paraffin-embedded tissue sections, a concentration of 1.0 µg/mL is suggested. For western blotting, the suggested use of this reagent is 0.01 - 0.5 µg/mL. It is recommended that the reagent to be titrated for optimal performance before each experiment.

Application Notes

Reactivity to Drosophila was only validated with the purified format.

RRID
AB_2832856 (BioLegend Cat. No. 819413)
AB_2832857 (BioLegend Cat. No. 819414)

Antigen Details

Structure
Histone proteins H3 and H4 bind to form a H3-H4 dimer, two of these H3-H4 dimers combine to form a tetramer. This tetramer further combines with two H2a-H2b dimers to form the compact Histone octamer core.
Distribution

Nucleus

Function
Histones play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability.
Interaction
Rsk-2, MSK1, GCN5 family of HATs, HDACs, PARP, SET7/9, and CARM1
Biology Area
Cell Biology, Chromatin Remodeling/Epigenetics, Neuroscience, Transcription Factors
Antigen References

1. Chen HM, et al. 2015. Epigenetics and Dermatology 409.

Gene ID
8350 View all products for this Gene ID
UniProt
View information about Histone H3 C-terminus on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 2    Revision Date: 04/02/2020

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

BioLegend Inc., 8999 BioLegend Way, San Diego, CA 92121 www.biolegend.com
Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

ProductsHere
Insert Note Here
Save Close Clear
Lab Timer
Tools
Login / Register
Forgot your password? Reset password?
Create an Account