Go-ChIP-Grade™ Purified anti-Bcl11b Antibody

Pricing & Availability
Clone
25B6 (See other available formats)
Other Names
B-cell CLL/lymphoma 11B (zinc finger protein), CTIP2, B-cell lymphoma/leukemia 11B
Isotype
Rat IgG2a, κ
Ave. Rating
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Product Citations
publications
25B6_GoChIP_Bcl11b_Antibody_1_ChIP_052317
Chromatin Immunoprecipitations (ChIP) were performed with cross-linked chromatin samples from 4 X 106 of Jurkat cells with either A)1:50 dilution of Go-ChIP-Grade™ Purified anti-Bcl11b (Clone 25B6, Cat. No 650603) or B) equal amount of Purified Rat IgG2a, κ Isotype Control Antibody (Clone RTK2758, Cat. No 400501) by using Go-ChIP-Grade™ Protein G Enzymatic Kit (Cat. No 699904). The enriched DNA was purified and quantified by real-time qPCR using primers targeting human CDKN1A gene region or &alpha-Satellite repeats. The amount of immunoprecipitated DNA in each sample is represented as signal relative to total amount of input chromatin.
  • 25B6_GoChIP_Bcl11b_Antibody_1_ChIP_052317
    Chromatin Immunoprecipitations (ChIP) were performed with cross-linked chromatin samples from 4 X 106 of Jurkat cells with either A)1:50 dilution of Go-ChIP-Grade™ Purified anti-Bcl11b (Clone 25B6, Cat. No 650603) or B) equal amount of Purified Rat IgG2a, κ Isotype Control Antibody (Clone RTK2758, Cat. No 400501) by using Go-ChIP-Grade™ Protein G Enzymatic Kit (Cat. No 699904). The enriched DNA was purified and quantified by real-time qPCR using primers targeting human CDKN1A gene region or &alpha-Satellite repeats. The amount of immunoprecipitated DNA in each sample is represented as signal relative to total amount of input chromatin.
  • 25B6_GoChIP_Bcl11b_Antibody_2_ChIP_052317
    Chromatin Immunoprecipitations (ChIP) were performed with cross-linked chromatin samples from 4 X 106 of Jurkat cells with either A)1:50 dilution of Go-ChIP-Grade™ Purified anti-Bcl11b (Clone 25B6, Cat. No 650603) or B) equal amount of Purified Rat IgG2a, κ Isotype Control Antibody (Clone RTK2758, Cat. No 400501) by using Go-ChIP-Grade™ Protein G Enzymatic Kit (Cat. No 699904). The enriched DNA was purified and quantified by real-time qPCR using primers targeting human CDKN1B gene region or α-Satellite repeats. The amount of immunoprecipitated DNA in each sample is represented as signal relative to total amount of input chromatin.
  • 25B6_GoChIP_Bcl11b_Antibody_2_WB_110317
    Total lysates (15 µg protein) from Jurkat (lane 1), HeLa (lane 2, negative control), and EL4 cells (lane 3) were resolved by electrophoresis (4-20% Tris-Glycine gel), transferred to nitrocellulose, and probed with 1:1000 diluted (0.5 µg/mL) Purified anti-Bcl11b Antibody, clone 25B6 (upper). Proteins were visualized by chemiluminescence detection using a 1:3000 diluted goat anti-rat-IgG secondary antibody conjugated to HRP for the anti-Bcl11b Antibody or 1:5000 diluted Direct-Blot HRP anti-β-Actin Antibody, clone 2F1-1(lower). Lane M: Molecular weight ladder.
  • 25B6_GoChIP_Bcl11b_Antibody_3_ICC_052317
    Jurkat cells were stained with anti-bcl11b, clone 25B6 (1:200 dilution), and tubulin-α, clone TU-01, followed by DyLight™ 549 conjugated anti-rat IgG and DyLight™ 488 conjugated anti-mouse IgG. Nuclei are stained with DAPI.
Cat # Size Price Quantity Avail. Save
650603 25 µg 240€
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650604 100 µg 560€
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Description

BCL11B is a C2H2-type zinc finger protein and is closely related to BCL11A whose translocation may be associated with B-cell malignancies. BCL11B is expressed in thymus, mainly T cells. It is indispensable for T lineage development. When BCL11B was deleted, T cells from all developmental stages acquired NK cell properties and concomitantly lost or decreased T cell-associated gene expression. These induced T-to-natural killer (ITNK) cells, which were morphologically and genetically similar to conventional NK cells, killed tumor cells in vitro, and effectively prevented tumor metastasis in vivo. Therefore, ITNKs may represent a new cell source for cell-based therapies. BCL11B is also known to be a transcriptional repressor, and is regulated by the NURD nucleosome remodeling and histone deacetylase complex. Recent research suggests that BCL11B is crucial for ameloblasts (the cells that produce tooth enamel) to form and work properly.

Product Details
Technical Data Sheet (pdf)

Product Details

Reactivity
Human, Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Human Bcl11b
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

ChIP - Quality tested
ICC, WB - Verified
IHC-P - Reported in the literature, not verified in house

Recommended Usage

Each lot of this antibody is quality control tested by ChIP Assay. The suggested dilution for ChIP application is 1:50-1:100 by volume. For western blotting, suggested working dilution(s): Use 0.5 µg antibody per ml antibody dilution buffer for each mini-gel. For immunofluorescence microscopy: Use a dilution of 1:200. The optimal concentration should be determined by titration for each individual assay of interest.

Application References

(PubMed link indicates BioLegend citation)
  1. Kosulin K, et al. 2013. PLoS One. 8:63646. PubMed. (IHC-P)
RRID
AB_2686936 (BioLegend Cat. No. 650603)
AB_2686935 (BioLegend Cat. No. 650604)

Antigen Details

Structure
894 amino acids with molecular weight of 96 kD. It encodes a C2H2-type zinc finger protein and is closely related to BCL11A.
Distribution

Nucleus

Function
Tumor-suppressor protein involved in T-cell lymphomas. A key regulator of both differentiation and survival during thymocyte development.
Interaction
Interacts with COUP1-TFI, SIRT1, ARP1, EAR2
Biology Area
Cell Biology, Immunology, Transcription Factors
Molecular Family
Nuclear Markers
Antigen References

1. Liu P, et al. 2011. Immunol. Rev. 238:138.
2. Ikawa T, et al. 2010. Science 329:93.
3. Li L, et al. 2010. Science 329:89.
4. Li P, et al. 2010. Science 329:85.
5. Tydell CC, et al. 2007. J. Immunol. 179:421.
6. Avram D, et al. 2002. Biochem. J. 368:555.
7. Golonzhka O, et al. 2009. Proc. Natl. Acad. Sci. U.S.A. 106:4278

Gene ID
64919 View all products for this Gene ID
UniProt
View information about Bcl11b on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 2    Revision Date: 05/07/2018

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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