FITC anti-human CD90 (Thy1) Antibody

Pricing & Availability
Clone
5E10 (See other available formats)
Regulatory Status
RUO
Workshop
HCDM listed
Other Names
Thy-1, Thy1
Isotype
Mouse IgG1, κ
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Product Citations
publications
5E10_FITC_020708
Human erythroleukemic cell line (HEL) stained with 5E10 FITC
  • 5E10_FITC_020708
    Human erythroleukemic cell line (HEL) stained with 5E10 FITC
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328107 25 tests 152€
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328108 100 tests 344€
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Description

CD90 is a 25-35 kD GPI-anchored protein, also known as Thy-1. It belongs to the Ig superfamily. Human CD90 is expressed on neuronal cells, a subset of CD34+ cells, a subset of fetal liver cells and fetal thymocytes, fibroblasts, activated endothelial cells, and some leukemia cell lines. CD34+CD90+ cells are primitive hematopoietic stem cells. It has been reported that Thy-1 binds with β2 and β3 integrins and plays bimodal roles in the regulation of cell adhesion and neurite outgrowth, and inhibits hematopoietic stem cells proliferation and differentiation.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human
Reported Reactivity
African Green, Baboon, Cynomolgus, Pigtailed Macaque, Rhesus, Pig
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
HEL cells
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation
The antibody was purified by affinity chromatography, and conjugated with FITC under optimal conditions.
Concentration
Lot-specific (to obtain lot-specific concentration, please enter the lot number in our Concentration and Expiration Lookup or Certificate of Analysis online tools.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.

Excitation Laser
Blue Laser (488 nm)
Application Notes

Clone 5E10 recognizes an epitope on Thy-1 independent of its glycosylation, but is abolished under reducing conditions.4 Additional reported (for the relevant formats) applications include: immunohistochemical staining of acetone-fixed frozen sections, immunoprecipitation1, and immunofluorescence3.

Application References

(PubMed link indicates BioLegend citation)
  1. Craig W, et al. 1993. J. Exp. Med. 177:1331. (IP)
  2. Gundlach CW 4th, et al. 2011. Bioconjug. Chem. 22:1706. (Pig Reactivity)
  3. Touboul C, et al. 2013. J. Transl. Med. 11:28. (IF)
  4. Bradley JE, et al. 2013. Lab Invest. 93:365. (Epitope)
  5. Donnenberg VS, et al. 2010. Cytometry B. Clin. Cytom. 5:287. (IHC)
Product Citations
  1. Camerlingo R, et al. 2011. Lung Cancer. 72:23. PubMed
  2. Meng Z, et al. 2013. Mol Cancer Ther. 12:2067. PubMed
  3. Aomatsu E, et al. 2014. Sci Rep. 4:3652. PubMed
  4. Li L, et al. 2015. Int J Pharmaceutics. 488: 1-11. PubMed
  5. Thiel A, et al. 2015. Sci Rep. 5: 17685. PubMed
  6. Zhang H, et al. 2017. Oncogene. 10.1038/onc.2016.512. PubMed
  7. Hanse E, et al. 2017. Oncogene. 10.1038/onc.2017.36. PubMed
  8. Noz MP, et al. 2020. Elife. 9:00. PubMed
  9. Luzuriaga J, et al. 2020. Biomedicines. 8:00. PubMed
  10. Peng K, et al. 2020. Sci Rep. 10:18433. PubMed
  11. Miksiunas R, et al. 2020. Int J Mol Sci. 21:00. PubMed
  12. Nguyen MAT, et al. 2020. Immunohorizons. 0.465277778. PubMed
  13. Yan H, et al. 2020. Adv Sci (Weinh). 7:1903516. PubMed
  14. Okamura G, et al. 2020. Int J Mol Sci. 22:00. PubMed
  15. DAgostino S, et al. 2021. Frontiers in Oncology. 10:600980. PubMed
  16. Ayhan S, et al. 2021. Journal of Cell Science. 134(6):. PubMed
  17. Tu S, et al. 2020. Cellular Signalling. 73:109695. PubMed
  18. Dinh HQ, et al. 2020. Immunity. 53(2):319-334.e6. PubMed
  19. Fawkner-Corbett D, et al. 2021. Cell. 184(3):810-826.e23. PubMed
  20. Tidu F, et al. 2021. iScience. 24(6):102683. PubMed
  21. Narakornsak S, et al. 2017. Acta Histochemica. 10.1016/j.acthis.2017.04.006. PubMed
  22. Motedayyen H, et al. 2017. BMC Res Notes. . 10.1186/s13104-017-2880-6. PubMed
  23. Zhu YP et al. 2018. Cell reports. 24(9):2329-2341 . PubMed
  24. Kinchen J et al. 2018. Cell. 175(2):372-386 . PubMed
  25. Yang C et al. 2019. Int J Mol Med. 43(3):1395-1405 . PubMed
  26. Zhang Y, et al. 2018. Stem Cells Int. 2018:7159465. PubMed
  27. Sun W, et al. 2019. Exp Ther Med. 17:2199. PubMed
  28. Gao P, et al. 2017. Cytotechnology. 69:751. PubMed
  29. He H, et al. 2019. J Cell Mol Med. 23:4139. PubMed
  30. Sprouse ML, et al. 2019. Int J Mol Sci. 20:8. PubMed
  31. Radhakrishnan S, et al. 2019. Mol Med Rep. 20:813. PubMed
  32. Vishnoi M, et al. 2019. Mol Oncol. 13(9): 1913. PubMed
  33. Nguyen JT, et al. 2019. iScience. 17:190. PubMed
  34. Vishnoi M, et al. 2018. Cancer Res. 78:5349. PubMed
  35. Sun T, et al. 2019. Haematologica. 105:661. PubMed
  36. Zhang Y, et al. 2019. Aging (Albany NY). 11:12641. PubMed
  37. Liu X, et al. 2020. Exp Ther Med. 1.258333333. PubMed
  38. Huang X, et al. 2019. Oxid Med Cell Longev. 2019:1305049. PubMed
  39. Qin XY, et al. 2020. Cell Death Dis. 0.504166667. PubMed
  40. Hong Y, et al. 2020. Aging Cell. 19:e13128. PubMed
  41. Han X, et al. 2020. Int J Oral Sci. 12:10. PubMed
  42. He Z, et al. 2020. J Exp Clin Cancer Res. 39:140. PubMed
  43. Yu C, et al. 2020. Sci Rep. 10:14521. PubMed
RRID
AB_893438 (BioLegend Cat. No. 328107)
AB_893429 (BioLegend Cat. No. 328108)

Antigen Details

Structure
25-35 kD glycoprotein, Ig superfamily
Distribution

Subset of CD34+ hematopoietic stem cells, subset of fetal thymocytes, subset of fetal liver cells, fibroblast, activated endothelial cells, neurons and some leukemia cell lines

Function
Regulate hematopoiesis and neural cell growth, cell adhesion
Ligand/Receptor
β3 integrin, β2 integrin
Cell Type
Endothelial cells, Fibroblasts, Hematopoietic stem and progenitors, Leukemia, Mesenchymal Stem Cells, Neurons, Thymocytes
Biology Area
Immunology, Stem Cells
Molecular Family
CD Molecules
Antigen References

1. McKenzie JL, et al. 1981. J. Immunol. 126:843.
2. Avalos AM, et al. 2002. Biol. Res. 35:231.
3. Wetzel A, et al. 2004. J. Immunol. 172:3850.

Gene ID
7070 View all products for this Gene ID
UniProt
View information about CD90 on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 3    Revision Date: 08/04/2014

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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