- 7/4 (See other available formats)
- Other Names
- GKPIPNPLLGLDST epitope tag
- Mouse IgG2b, κ
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Epitope tags are useful for the labeling and detection of proteins using immunoblotting, immunoprecipitation, and immunostaining techniques. The V5 tag is a 14 amino acid peptide derived from a small epitope (Pk) on the P and V proteins of simian virus 5 (SV5), a member of the paramyxovirus family. Because of its small size, the addition of a V5 tag sequence (GKPIPNPLLGLDST) to a given gene creates a stable fusion product that does not appear to interfere with the biochemical properties of the protein. Many recombinant proteins have been engineered to express a short sequence derived from the V5 molecule known as V5-tag.Product Details
- GKPIPNPLLGLDST tag epitope
- Antibody Type
- Host Species
- This antibody is provided in 50% glycerol in aqueous buffered solutions with preservatives.
- The antibody was purified by affinity chromatography and conjugated with HRP under optimal conditions.
- Lot-specific (please contact technical support for concentration and total µg amount, or use our Lookup tool if you have a lot number.)
- Storage & Handling
- Upon receipt, the antibody solution should be stored undiluted at -20°C, and protected from prolonged exposure to light.
WB - Quality tested
- Recommended Usage
Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested dilution is 1:1000-1:2000. The optimal dilution should be determined by titration for each individual assay of interest.
25 µl and 100 µl of Direct-Blot™ HRP antibody can be used for approximately 5 and 20 Western blots, respectively, at the recommended concentration/dilution.
AB_2734532 (BioLegend Cat. No. 680604)
AB_2572008 (BioLegend Cat. No. 680603)
- 14 amino acid peptide (GKPIPNPLLGLDST)
- The V5 tag can be used to detect the expression of recombinant proteins in bacteria, yeast, insects, and mammalian cell systems.
- Biology Area
- Cell Biology
- Antigen References
1. Uhlen M, et al. 1990. Meth Enzymol. 185:129.
2. Hanke T, et al. 1995. J. Virol. Methods 53:149.
3. Southern JA, et al. 1991. J. Gen. Virol. 72:1551.
4. Thomas SM, et al. 1988. Cell 54:891.
- Gene ID
- View information about V5-tag on UniProt.org
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