Direct-Blot™ HRP anti-Granzyme B Antibody

Pricing & Availability
Clone
M3304B06 (See other available formats)
Other Names
Granzyme 2, cytotoxic T-lymphocyte-associated serine esterase 1, GZMB, CCP1, Asp-ase, CSPB, CTLA-1, CGL-1, CGL1
Isotype
Mouse IgG1
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Product Citations
publications
M3304B06_DBHRP_GranzymeB_Antibody_051017
Total cell lysate from HeLa (lane 1, negative control) and PBMC (lane 2) (15 µg/lane) were resolved by electrophoresis (4-12% Bis-Tris), transferred to nitrocellulose, and probed with 1:250 purified anti-Granzyme B (clone M3304B06) and 1:1000, 1:2500 and 1:10000 Direct-Blot™ HRP anti- Granzyme B Antibody(upper) or loading control GAPDH (poly6314) antibody (lower). Proteins were visualized by chemiluminescence detection using a goat anti-mouse-IgG secondary antibody conjugated to HRP for the purified antibody, and a donkey anti-rabbit IgG Antibody conjugated to HRP for GAPDH. Lane M is the molecular weight ladder.
  • M3304B06_DBHRP_GranzymeB_Antibody_051017
    Total cell lysate from HeLa (lane 1, negative control) and PBMC (lane 2) (15 µg/lane) were resolved by electrophoresis (4-12% Bis-Tris), transferred to nitrocellulose, and probed with 1:250 purified anti-Granzyme B (clone M3304B06) and 1:1000, 1:2500 and 1:10000 Direct-Blot™ HRP anti- Granzyme B Antibody(upper) or loading control GAPDH (poly6314) antibody (lower). Proteins were visualized by chemiluminescence detection using a goat anti-mouse-IgG secondary antibody conjugated to HRP for the purified antibody, and a donkey anti-rabbit IgG Antibody conjugated to HRP for GAPDH. Lane M is the molecular weight ladder.
Cat # Size Price Quantity Avail. Save
674603 100 µl 464€
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Description

Granzyme B is a serine protease that is mainly produced by cytotoxic T cells (CTL) and NK cells. It plays important roles in cytotoxic lymphocyte-mediated apoptosis, chronic inflammation, and impaired wound healing. Granzyme B is packaged in cytoplasmic granules that are exocytosed towards a bound target cell, and subsequently activates multiple protein substrates to induce apoptosis. Perforin facilitates the transfer of Granzyme B into the target cell. Most circulating CD56+ CD8- NK cells and approximately half of circulating CD8+ T cells coexpress both Granzyme A and B. The activation of CD8+ and CD4+ T lymphocytes induces substantial expression of Granzyme B, but not Granzyme A. Recently, Granzyme B has been found to be produced by other cell types such as CD34+ hematopoietic progenitor cells, keratinocytes, basophils, mast cells, plasmacytoid dendritic cells, B cells, and smooth muscle cells. Elevated levels of Granzyme B are also found in some kinds of autoimmune diseases, type 1 diabetes, and cardiovascular diseases.

Product Details
Technical Data Sheet (pdf)

Product Details

Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Recombinant human Granzyme B produced in the 293E cell line.
Formulation
This antibody is provided in 50% glycerol in aqueous buffered solutions with preservatives.
Preparation
The antibody was purified by affinity chromatography and conjugated with HRP under optimal conditions.
Concentration
Lot-specific (please contact technical support for concentration and total µg amount, or use our Lookup tool if you have a lot number.)
Storage & Handling
Upon receipt, the antibody solution should be stored undiluted at -20°C, and protected from prolonged exposure to light.
Application

WB - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested dilution is 1:1,000-1:10.000. The optimal dilution should be determined by titration for each individual assay of interest.

25 µl and 100 µl of Direct-Blot™ HRP antibody can be used for approximately 5 and 20 Western blots, respectively, at the recommended concentration/dilution.

Application Notes

This antibody can be used for at least 20 western blots.

RRID
AB_2687057 (BioLegend Cat. No. 674603)

Antigen Details

Structure
247 amino acids with a molecular weight of approximately 28 kD
Distribution

Cytotoxic T cells, NK cells, and neutrophils.

Function
Granzyme B is able to induce target cell apoptosis by activating caspase independent pathways. Granzyme B is induced in CD8+ T lymphocytes with ConA / IL-2 and CD4+ T lymphocytes with anti-CD3/CD28 or anti-CD3/CD46.
Interaction
Targets of CTL and NK cells.
Cell Type
T cells, NK cells, Neutrophils, B cells, Dendritic cells
Biology Area
Apoptosis/Tumor Suppressors/Cell Death, Cell Biology, Cell Cycle/DNA Replication, Immunology, Innate Immunity, Neuroscience, Signal Transduction
Molecular Family
Proteases, Enzymes and Regulators
Antigen References

1. Edwards KM, et al. 1999. J. Biol. Chem. 274:30468.
2. Grossman WJ, et al. 2004. Blood 104:2840.
3. Heusel JW, et al. 1994. Cell 76:977.
4. Schmid J and Weissmann C. 1987. J. Immunol. 139:250.
5. Trapani JA, et al. 1988. Proc. Natl. Acad. Sci. USA 85:6924.
6. Hiebert PR and Granville DJ. 2012. Trends Mol. Med. 18:732.
7. Saito Y, et al. 2011. J. Cardiol. 57:141.
8. Ewen CL, et al. 2012. Cell Death Differ. 1:28.

Gene ID
3002 View all products for this Gene ID
UniProt
View information about Granzyme B on UniProt.org

Related FAQs

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Go To Top Version: 0    Revision Date: 05/12/2017

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Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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