Cytokeratin (OSCAR) Monoclonal Antibody, Purified

Pricing & Availability
Clone
OSCAR (See other available formats)
Isotype
Mouse IgG2a
OSCAR_Purified_Cytokeratin_Antibody_IHC_030315
Immunostaining of squamous cells surrounding sebaceous cells in skin using the anti-cytokeratin antibody (clone OSCAR).
  • OSCAR_Purified_Cytokeratin_Antibody_IHC_030315
    Immunostaining of squamous cells surrounding sebaceous cells in skin using the anti-cytokeratin antibody (clone OSCAR).
Cat # Size Price Quantity Avail. Save
908203 6 mL 322€
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908202 6 mL 387€
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908201 1 mL 590€
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Description

This antibody is effective in immunohistochemistry (IHC).

BioLegend’s anti-cytokeratin antibody clone OSCAR demonstrates a broad spectrum of cytokeratin reactivity. OSCAR is reactive with most epithelial types (in normal tissues) and with most carcinomas.

Concentrated Format:
Cat. No. 908201 (Formerly: SIG-3465-1000) [1 ml]

Prediluted Formats: 
Cat. No. 908202 (Formerly: SIG-3465-16) [6 ml] Ready-to-use with automated stainers with a Biotin-based (USA Ultra Streptavidin Detection, Cat. No. 929501) detection system.

Cat. No. 908203 (Formerly: SIG-3465-26) [6 ml] Ready-to-use with manual staining with a Biotin-based (USA Ultra Streptavidin Detection, Cat. No. 929501) detection system.

Product Details
Instructions for Use (pdf)

Product Details

Product Information

Intended Use:

In Vitro Diagnostic (IVD). Use in immunohistochemistry (IHC) test methods only.

The monoclonal antibody OSCAR is used for the in vitro examination of human biological samples using immunohistochemistry (IHC) methods for the qualitative identification of Cytokeratin. The clinical interpretation of any staining or its absence should be complemented by morphological studies and proper controls and should be evaluated within the context of the patient's clinical history and other diagnostic tests by a qualified pathologist.

Reactivity
Human
Immunogen
This antibody was developed using a crude cytokeratin extract prepared from RT-4 and MCF-7 cells.
Formulation
Phosphate-buffered solution with BSA + 0.1% NaN3.
Preparation
The antibody was purified by affinity chromatography.
Storage & Handling
Store between 2°C and 8°C.
Recommended Usage

Each lot of this antibody is quality control tested by immunohistochemical staining.

The optimal working dilution should be determined for each specific assay condition.
IHC: ≥1:40 (concentrated format) with Biotin based detection systems such as USA Ultra Streptavidin Detection (Cat. No. 929501).

Positive tissue (human): Skin, Liver, Bladder, Breast

Tissue Sections: Formalin-fixed, paraffin-embedded tissues, frozen sections
Pretreatment: For optimal staining, the sections should be pretreated with an antigen unmasking solution such as citrate buffer retrieval solution (Cat. No. 928502).
Incubation: 20 minutes at room temperature

Application References

(PubMed link indicates BioLegend citation)
  1. Bahrami A, Gown AM, Baird GS, Hicks MJ, Folpe AL. Aberrant expression of epithelial and neuroendocrine markers in alveolar rhabdomyosarcoma: a potentially serious diagnostic pitfall. Mod Pathol 21(7):795-806, 2008.
  2. Gown, AM, Yaziji H, Barry TS, Hwang HC. OSCAR, A novel broad anti-cytokeratin monoclonal antibody optimized for diagnostic immunohistochemistry. US Canadian Acad Pathol Ann Meeting, 2003.
Product Citations
  1. Drummond CJ et al. 2018. Cancer cell. 33(1):108-124 . PubMed
RRID
AB_2565083 (BioLegend Cat. No. 908203)
AB_2565082 (BioLegend Cat. No. 908202)
AB_2565081 (BioLegend Cat. No. 908201)
Disclaimer

WARNINGS AND PRECAUTIONS

  1. Use appropriate personal protective equipment and safety practices per universal precautions when working with this reagent. Refer to the reagent safety data sheet.
  2. This antibody contains sodium azide. Follow federal, state and local regulations to dispose of this reagent. Sodium azide build-up in metal wastepipes may lead to explosive conditions; if disposing of reagent down wastepipes, flush with water after disposal.
  3. All specimens, samples and any material coming in contact with them should be considered potentially infectious and should be disposed of with proper precautions and in accordance with federal, state and local regulations.
  4. Do not use this reagent beyond the expiration date stated on the label.
  5. Do not use this reagent if it appears cloudy or if there is any change in the appearance of the reagent as these may be indication of possible deterioration.

Related FAQs

There are no FAQs for this product.
Go To Top Version: 6    Revision Date: 06/10/2020

Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

BioLegend Inc., 8999 BioLegend Way, San Diego, CA 92121 www.biolegend.com
Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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