- OSCAR (See other available formats)
- Other Names
- Osteoclast associated receptor (OSCAR)
Signet Catalog# 465-01
Signet Catalog# 465-16 L1 Predilute
Signet Catalog# 465-26 L2 Predilute
Covance Catalog# SIG-3465
- Mouse IgG2a
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- Product Citations
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- Intended Use
In Vitro Diagnostic (IVD). Use in immunohistochemistry (IHC) test methods only.
The monoclonal antibody OSCAR is used for the in vitro examination of human biological samples using immunohistochemistry (IHC) methods for the qualitative identification of Cytokeratin. The clinical interpretation of any staining or its absence should be complemented by morphological studies and proper controls and should be evaluated within the context of the patient's clinical history and other diagnostic tests by a qualified pathologist.
- Human, Dog, Primate
- Antibody Type
- Host Species
- This antibody was developed using a crude cytokeratin extract prepared from RT-4 and MCF-7 cells.
- Phosphate-buffered solution with BSA + 0.1% NaN3.
- The antibody was purified by affinity chromatography.
- Storage & Handling
- Store between 2°C and 8°C.
- Recommended Usage
Each lot of this antibody is quality control tested by immunohistochemical staining.
The optimal working dilution should be determined for each specific assay condition.
• IHC: ≥1:40 (concentrated format) with Biotin based detection systems such as USA Ultra Streptavidin Detection (Cat. No. 929501).
Positive tissue (human): Skin, Liver, Bladder, Breast
Tissue Sections: Formalin-fixed, paraffin-embedded tissues, frozen sections
Pretreatment: For optimal staining, the sections should be pretreated with an antigen unmasking solution such as citrate buffer retrieval solution (Cat. No. 928502, 928602).
Incubation: 20 minutes at room temperature
Precautions for Use: Use appropriate personal protective equipment and safety practices per universal precautions when working with this reagent. Refer to the reagent safety data sheet.
- Application Notes
This antibody is effective in immunohistochemistry (IHC).
BioLegend’s anti-cytokeratin antibody clone OSCAR demonstrates a broad spectrum of cytokeratin reactivity. OSCAR is reactive with most epithelial types (in normal tissues) and with most carcinomas.
Cat. No. 908201 (Formerly: SIG-3465-1000) [1 ml]
Cat. No. 908202 (Formerly: SIG-3465-16) [6 ml] Ready-to-use with automated stainers with a Biotin-based (USA Ultra Streptavidin Detection, Cat. No. 929501) detection system.
Cat. No. 908203 (Formerly: SIG-3465-26) [6 ml] Ready-to-use with manual staining with a Biotin-based (USA Ultra Streptavidin Detection, Cat. No. 929501) detection system.
(PubMed link indicates BioLegend citation)
- Bahrami A, Gown AM, Baird GS, Hicks MJ, Folpe AL. Aberrant expression of epithelial and neuroendocrine markers in alveolar rhabdomyosarcoma: a potentially serious diagnostic pitfall. Mod Pathol 21(7):795-806, 2008.
- Gown, AM, Yaziji H, Barry TS, Hwang HC. OSCAR, A novel broad anti-cytokeratin monoclonal antibody optimized for diagnostic immunohistochemistry. US Canadian Acad Pathol Ann Meeting, 2003.
- Product Citations
AB_2565083 (BioLegend Cat. No. 908203)
AB_2565082 (BioLegend Cat. No. 908202)
AB_2565081 (BioLegend Cat. No. 908201)
- Biology Area
- Cell Biology, Cell Motility/Cytoskeleton/Structure, Neuroscience, Neuroscience Cell Markers
- Molecular Family
- Intermediate Filaments
- Gene ID
- View information about Cytokeratin on UniProt.org
- Does BioLegend manufacture antibodies at GMP grade?
BioLegend antibodies and reagents that carry the IVD or ASR designation are manufactured in a registered GMP facility, while our other products are manufactured under ISO 13485 certification. Both environments provide high quality products. To learn more about our quality standards visit our Quality Control page at: https://www.biolegend.com/QC.
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