Cytokeratin (AE-1) Monoclonal Antibody, Purified

Pricing & Availability
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AE-1 (See other available formats)
Other Names
Cytokeratin (AE1)
Previously
Signet Catalog# 462-01
Covance Catalog# SIG-3462
Isotype
Mouse IgG1
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Cat # Size Price Quantity Avail. Save
914001 1 ml 322€
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Instructions for Use (pdf)

Product Details

Intended Use
In Vitro Diagnostic (IVD). Use in immunohistochemistry (IHC) test methods only.

The monoclonal antibody AE-1 is used for the in vitro examination of human biological samples using immunohistochemistry (IHC) methods for the qualitative identification of Cytokeratin. The clinical interpretation of any staining or its absence should be complemented by morphological studies and proper controls and should be evaluated within the context of the patient's clinical history and other diagnostic tests by a qualified pathologist.
Reactivity
Rat, Dog, Human, Primate
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
This antibody was developed using human epidermal keratins.
Formulation
Phosphate-buffered solution with BSA + 0.1% NaN3.
Preparation
The antibody was purified by affinity chromatography.
Storage & Handling
Store between 2°C and 8°C.
Application

IHC

Recommended Usage

Each lot of this antibody is quality control tested by immunohistochemical staining.

The optimal working dilution should be determined for each specific assay condition.
IHC: ≥1:40 (concentrated format) with Biotin based detection systems such as USA Ultra Streptavidin Detection (Cat. No. 929501).

Positive tissue (human): Skin

Tissue Sections: Formalin-fixed, paraffin-embedded tissues
Pretreatment: For optimal staining, the sections should be pretreated with an antigen unmasking solution such as Retrieve-All 1 (Cat. No. 927901).
Incubation: 20 minutes at room temperature

Precautions for Use: Use appropriate personal protective equipment and safety practices per universal precautions when working with this reagent. Refer to the reagent safety data sheet.

Application Notes

This antibody is effective in immunohistochemistry (IHC).

The cytokeratin AE-1 antibody recognizes low and intermediate molecular weight type I acidic keratins (40, 48, 50, and 56.5 kD). Cytokeratin AE-1 stains the basal layer of epidermis and most epithelia.

Concentrated Format:
Cat. No. 914001 (Formerly: SIG-3462-1000) [1 mL]

RRID
AB_2565148 (BioLegend Cat. No. 914001)

Antigen Details

Biology Area
Cell Biology, Cell Motility/Cytoskeleton/Structure, Neuroscience, Neuroscience Cell Markers
Molecular Family
Intermediate Filaments
Gene ID
NA
UniProt
View information about Cytokeratin on UniProt.org

Related FAQs

Does BioLegend manufacture antibodies at GMP grade?

BioLegend antibodies and reagents that carry the IVD or ASR designation are manufactured in a registered GMP facility, while our other products are manufactured under ISO 13485 certification. Both environments provide high quality products. To learn more about our quality standards visit our Quality Control page at: https://www.biolegend.com/QC.

Go To Top Version: 4    Revision Date: 05/11/2017

Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

BioLegend Inc., 8999 BioLegend Way, San Diego, CA 92121 www.biolegend.com
Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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