- AE-1 (See other available formats)
- Other Names
- Cytokeratin (AE1)
Signet Catalog# 462-01
Covance Catalog# SIG-3462
- Mouse IgG1
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- Intended Use
In Vitro Diagnostic (IVD). Use in immunohistochemistry (IHC) test methods only.
The monoclonal antibody AE-1 is used for the in vitro examination of human biological samples using immunohistochemistry (IHC) methods for the qualitative identification of Cytokeratin. The clinical interpretation of any staining or its absence should be complemented by morphological studies and proper controls and should be evaluated within the context of the patient's clinical history and other diagnostic tests by a qualified pathologist.
- Rat, Dog, Human, Primate
- Antibody Type
- Host Species
- This antibody was developed using human epidermal keratins.
- Phosphate-buffered solution with BSA + 0.1% NaN3.
- The antibody was purified by affinity chromatography.
- Storage & Handling
- Store between 2°C and 8°C.
- Recommended Usage
Each lot of this antibody is quality control tested by immunohistochemical staining.
The optimal working dilution should be determined for each specific assay condition.
• IHC: ≥1:40 (concentrated format) with Biotin based detection systems such as USA Ultra Streptavidin Detection (Cat. No. 929501).
Positive tissue (human): Skin
Tissue Sections: Formalin-fixed, paraffin-embedded tissues
Pretreatment: For optimal staining, the sections should be pretreated with an antigen unmasking solution such as Retrieve-All 1 (Cat. No. 927901).
Incubation: 20 minutes at room temperature
Precautions for Use: Use appropriate personal protective equipment and safety practices per universal precautions when working with this reagent. Refer to the reagent safety data sheet.
- Application Notes
This antibody is effective in immunohistochemistry (IHC).
The cytokeratin AE-1 antibody recognizes low and intermediate molecular weight type I acidic keratins (40, 48, 50, and 56.5 kD). Cytokeratin AE-1 stains the basal layer of epidermis and most epithelia.
Cat. No. 914001 (Formerly: SIG-3462-1000) [1 mL]
AB_2565148 (BioLegend Cat. No. 914001)
- Biology Area
- Cell Biology, Cell Motility/Cytoskeleton/Structure, Neuroscience, Neuroscience Cell Markers
- Molecular Family
- Intermediate Filaments
- Gene ID
- View information about Cytokeratin on UniProt.org
- Does BioLegend manufacture antibodies at GMP grade?
BioLegend antibodies and reagents that carry the IVD or ASR designation are manufactured in a registered GMP facility, while our other products are manufactured under ISO 13485 certification. Both environments provide high quality products. To learn more about our quality standards visit our Quality Control page at: https://www.biolegend.com/QC.
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