Cytokeratin 8 Monoclonal Antibody, Purified

Pricing & Availability
Clone
1E8 (See other available formats)
Isotype
Mouse IgG2a
1E8_Cytokeratin-8_Antibody_IHC_021315
Immunohistochemistry staining with cytokeratin-8 (clone 1E8) showing strong cytoplasmic staining of the ducts in normal human skin tissue.
  • 1E8_Cytokeratin-8_Antibody_IHC_021315
    Immunohistochemistry staining with cytokeratin-8 (clone 1E8) showing strong cytoplasmic staining of the ducts in normal human skin tissue.
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904801 250 µL 598€
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Description

Cytokeratin-8 copolymerizes with cytokeratin-18 to form a component of the cytoskeleton in simple epithelia and many epithelial cell derived cancers.

Product Details
Instructions for Use (pdf)

Product Details

Product Information

Intended Use:
In Vitro Diagnostic (IVD). Use in immunohistochemistry (IHC) test methods only.

The monoclonal antibody 1E8 is used for the in vitro examination of human biological samples using immunohistochemistry (IHC) methods for the qualitative identification of Cytokeratin 8. The clinical interpretation of any staining or its absence should be complemented by morphological studies and proper controls and should be evaluated within the context of the patient's clinical history and other diagnostic tests by a qualified pathologist.

Reactivity
Human, Mouse, Dog, Primate
Immunogen
Antibody 1E8 was raised against the 12 carboxy-terminal amino acids of HK-8 preceded by a cysteine, CKLVSESSDVLPK.
Formulation
Phosphate-buffered solution + 0.03% Thimerosal.
Preparation
The antibody was purified by affinity chromatography.
Concentration
1.0 mg/ml
Storage & Handling
When stored at ≤ -20°C, product is stable until the date shown on the label. Avoid repeated freeze-thaw cycles to prevent denaturing the antibody. If thawed and stored at 2-8°C, product is stable for 24 months from the date of thaw or until the expiry date on the label, whichever comes first.
Recommended Usage

Each lot of this antibody is quality control tested by immunohistochemical staining.

The optimal working dilution should be determined for each specific assay condition.
IHC: 1:1,000 with either biotin based detection systems such as USA Ultra Streptavidin Detection (Cat. No. 929501).

Tissue Sections: Paraffin-embedded tissues
Pretreatment: For optimal staining, the sections should be pretreated with an antigen unmasking solution such as Citrate Buffer Retrieval solution (Cat. No. 928502, 928602).
Incubation: 60 minutes at room temperature

Precautions for Use: Use appropriate personal protective equipment and safety practices per universal precautions when working with this reagent. Refer to the reagent safety data sheet.

Application References

(PubMed link indicates BioLegend citation)
  1. Memarzadeh S, Zong Y, Janzen DM, Goldstein AS, Cheng D, Kurita T, Schafenacker AM, Huang J, and Witte ON. Cell-autonomous activation of the PI3-kinase pathway initiates endometrial cancer from adult uterine epithelium. PNAS, 107: 17298-17303, Oct 2010. [IHC]
  2. Hembrough TA, Li L, Gonias SL. Cell surface cytokeratin-8 is the major plasminogen receptor on breast cancer cells and is required for the accelerated activation of cell-associated plasminogen by tissue-type plasminogen activator. J Biol Chem 271:25684-25691, 1996.
  3. Hübner A, et al. JNK and PTEN cooperatively control the development of invasive adenocarcinoma of the prostate. PNAS, 109: 12046-12051, Jul 2012. [IHC] PubMed
  4. Easter SL, et al. 2014. PLoS One. 9:113247. (IF, WB) PubMed
Product Citations
  1. Hübner A, et al. 2012. Proc Natl Acad Sci U S A. 109:12046-12051. PubMed
  2. Easter S, et al. 2014. PLoS One. 9:e113247. PubMed
  3. Johnson D, et al. 2016. PLoS One. 11: 0148851. PubMed
  4. Park J, et al. 2016. Proc Natl Acad Sci U S A. 113: 4482-4487. PubMed
  5. Linn D, et al. 2016. Cancer Res . 76: 1869-1881. PubMed
  6. Kwon O, et al. 2016. Stem Cell Res. 16: 682-691. PubMed
  7. Wang N, et al. 2016. Stem Cell Reports. 6: 668-678. PubMed
  8. Gao D, et al. 2016. PLoS One. 11: 0161084. PubMed
  9. Chiche A, et al. 2016. Oncogene. 10.1038/onc.2016.396. PubMed
  10. Wu M, et al. 2016. J Biol Chem. 291(49):25749-25760. PubMed
  11. Tao L, et al. 2017. Nat Commun. 8:14431. PubMed
  12. Ai J, et al. 2017. Prostate. 10.1002/pros.23386. PubMed
  13. Li Q, et al. 2018. Neoplasia. 20:233. PubMed
  14. Chiche A, et al. 2019. Breast Cancer Res. 21:13. PubMed
  15. Barros-Silva JD, et al. 2018. Cell Rep. 25:3504. PubMed
  16. Lee DH, et al. 2019. PLoS One. 14:e0211153. PubMed
  17. Jiao X, et al. 2019. Stem Cell Reports. 12:135. PubMed
  18. Yang CS, et al. 2017. Prostate. 77:1452. PubMed
  19. Li Q, et al. 2018. J Biol Chem. 293:6434. PubMed
  20. Kwon OJ, et al. 2019. iScience. 13:328. PubMed
  21. He Y, et al. 2018. Stem Cells. 36:891. PubMed
  22. Bresson L, et al. 2018. Development. 145. PubMed
  23. Livshits G et al. 2018. eLife. 7 pii: e35216. PubMed
  24. Xiong X et al. 2018. Cell reports. 25(11):3006-3020 . PubMed
Publication Library
RRID
AB_2565043 (BioLegend Cat. No. 904801)

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*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

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Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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