- LG.3A10 (See other available formats)
- Regulatory Status
- Other Names
- T14, S152, Tp55, TNFRSF7
- Armenian Hamster IgG
- Ave. Rating
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- Product Citations
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CD27 is also known as S152 and T14. A member of the tumor necrosis factor receptor (TNFR) superfamily, it is a 45 kD protein expressed on peripheral T cells, memory B cells, NK cells, and thymocyte subset. Through its ligand, CD70, CD27 plays a key role in T cell and B cell interactions. Additionally, ligation of CD27 on naïve T cells may be important in their maturation to effector cells.Product Details
- Mouse, Rat, Human
- Antibody Type
- Host Species
- Armenian Hamster
- Armenian hamster fibroblast line ARHO12 transfected with mouse CD27 cDNA
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA).
- The antibody was purified by affinity chromatography and conjugated with Brilliant Violet 785™ under optimal conditions.
- 0.2 mg/ml
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
FC - Quality tested
- Recommended Usage
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.
Brilliant Violet 785™ excites at 405 nm and emits at 785 nm. The bandpass filter 780/60 nm is recommended for detection, although filter optimization may be required depending on other fluorophores used. Be sure to verify that your cytometer configuration and software setup are appropriate for detecting this channel. Refer to your instrument manual or manufacturer for support. Brilliant Violet 785™ is a trademark of Sirigen Group Ltd.
Learn more about Brilliant Violet™.
This product is subject to proprietary rights of Sirigen Inc. and is made and sold under license from Sirigen Inc. The purchase of this product conveys to the buyer a non-transferable right to use the purchased product for research purposes only. This product may not be resold or incorporated in any manner into another product for resale. Any use for therapeutics or diagnostics is strictly prohibited. This product is covered by U.S. Patent(s), pending patent applications and foreign equivalents.
- Excitation Laser
Violet Laser (405 nm)
- Application Notes
Additional reported applications (for the relevant formats) include: functional assay1,3 and immunohistomecial staining of acetone-fixed frozen sections2. The Ultra-LEAF™ purified antibody (Endotoxin < 0.01 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. Nos. 124243 & 124244).
(PubMed link indicates BioLegend citation)
- Gravestein LA, et al. 1995. Int. Immunol. 7:551. (FA)
- Gravestein LA, et al. 1996. J. Exp. Med. 184:675. (IHC)
- Takeda K, et al. 2000. J. Immunol. 164:1741. (FA)
- Welner RS, et al. 2009. J. Immunol. 183:7768. PubMed
- Vicetti Miguel RC, et al. 2012. J. Immunol. 189:3449. PubMed
- White CA, et al. 2014. J Immunol. 193:5933. PubMed
- Iwata S, et al. 2015. Lupus. 24:695. PubMed
AB_2800595 (BioLegend Cat. No. 124241)
- TNFR superfamily, 45 kD
Peripheral blood T cells, memory B cells, NK cells, thymocyte subset
- Costimulatory signal for T and B cell activation, T cell development
- TRAF2, TRAF5, CD27-binding protein (Siva)
- Cell Type
- B cells, NK cells, T cells, Thymocytes
- Biology Area
- Costimulatory Molecules, Immunology
- Molecular Family
- CD Molecules
- Antigen References
1. Hintzen RQ, et al. 1994. Immunol. Today 15:307.
2. Akiba H, et al. 1998. J. Biol. Chem. 273:13353.
3. Kobata T, et al. 1995. P. Natl. Acad. Sci. USA 92:11249.
4. Hendriks J, et al. 2000. Nat. Immunol. 1:433
5. Hintzen RQ, et al. 1994. Int. Immunol. 6:477
6. Camerini D, et al. 1991. J. Immunol. 147:3165
- Gene ID
- 21940 View all products for this Gene ID 939 View all products for this Gene ID 500318 View all products for this Gene ID
- View information about CD27 on UniProt.org
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