Brilliant Violet 421™ anti-Oct4 (Oct3) Antibody

Pricing & Availability
Clone
3A2A20 (See other available formats)
Other Names
POU domain class 5, transcription factor 1 (POU5F1), OCT4, OCT3
Isotype
Mouse IgG2b, κ
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Product Citations
publications
1_3A2A20_BV421_Oct4_Antibody_ICC_080219
NCCIT pluripotent embryonal carcinoma cells were fixed with 4% paraformaldehyde (PFA) for ten minutes, permeabilized with 0.5% Triton X-100 for ten minutes, and blocked with 5% FBS for 30 minutes. Then the cells were intracellularly stained with 5 µg/ml anti-Oct4 (Oct3) (clone 3A2A20) Brilliant Violet 421™ (blue) in 5% FBS overnight at 4°C and followed by Alexa Fluor® 488 Phalloidin (green) staining for 20 minutes at 4°C. Nuclei were counterstained with DRAQ5 (red). The image was captured with a 40X objective.
  • 1_3A2A20_BV421_Oct4_Antibody_ICC_080219
    NCCIT pluripotent embryonal carcinoma cells were fixed with 4% paraformaldehyde (PFA) for ten minutes, permeabilized with 0.5% Triton X-100 for ten minutes, and blocked with 5% FBS for 30 minutes. Then the cells were intracellularly stained with 5 µg/ml anti-Oct4 (Oct3) (clone 3A2A20) Brilliant Violet 421™ (blue) in 5% FBS overnight at 4°C and followed by Alexa Fluor® 488 Phalloidin (green) staining for 20 minutes at 4°C. Nuclei were counterstained with DRAQ5 (red). The image was captured with a 40X objective.
  • 2_3A2A20_BV421_Oct4_Antibody_ICFC_060617
    Human teratocarcinoma cell line NCCIT cells were fixed and permeabilized with BioLegend’s FOXP3 Fix/Perm Buffer Set, and then stained with anti-Oct4 (clone 3A2A20) Brilliant Violet 421™ (filled histogram) or mouse IgG2b, κ Brilliant Violet 421™ isotype control (open histogram).
See Brilliant Violet 421™ spectral data
Cat # Size Price Quantity Avail. Save
653712 100 µg 632€
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Description

Oct4 (Octamer binding transcription factor 4) is an important marker of the undifferentiated state and central regulator of pluripotency in ES cells. When embryonic stem cells are triggered to differentiate, Oct4 is downregulated, thus providing a model for the early events linked to somatic differentiation in the developing embryo. Oct4 is a nuclear protein, with a predicted molecular weight of 39 kD.

Product Details
Technical Data Sheet (pdf)

Product Details

Reactivity
Human, Mouse
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Partial human Oct4 recombinant protein (1-141aa).
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA).
Preparation
The antibody was purified by affinity chromatography and conjugated with Brilliant Violet 421™ under optimal conditions.
Concentration
0.2 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

ICC - Quality tested
ICFC - Validated

Recommended Usage

Each lot of this antibody is quality control tested by immunocytochemistry. For immunocytochemistry, a concentration range of 0.1 - 10 μg/ml is recommended. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 μg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Brilliant Violet 421™ excites at 405 nm and emits at 421 nm. The standard bandpass filter 450/50 nm is recommended for detection. Brilliant Violet 421™ is a trademark of Sirigen Group Ltd.


Learn more about Brilliant Violet™.

This product is subject to proprietary rights of Sirigen Inc. and is made and sold under license from Sirigen Inc. The purchase of this product conveys to the buyer a non-transferable right to use the purchased product for research purposes only. This product may not be resold or incorporated in any manner into another product for resale. Any use for therapeutics or diagnostics is strictly prohibited. This product is covered by U.S. Patent(s), pending patent applications and foreign equivalents.
Excitation Laser
Violet Laser (405 nm)
Application Notes

Clone 3A2A20 has been found to weakly cross react with mouse.

RRID
AB_2566690 (BioLegend Cat. No. 653712)

Antigen Details

Structure
Belongs to the POU transcription factor family, predicted molecular weight of 39 kD
Distribution

Nuclear protein, expressed in undifferentiated embryonal carcinoma (EC) cells, embryonic stem (ES) cells and embryonic germ (EG) cells

Function
Transcription factor that binds to the octamer motif (5'-ATTTGCAT-3'). Oct4 is a prime candidate for an early developmental control gene. It also is an important marker of the undifferentiated state and central regulator of pluripotency in ES cells.
Interaction
SOX2, SOX8, SOX10, androgen receptor, and ETS2
Biology Area
Cell Biology, Immunology, Stem Cells, Transcription Factors
Antigen References

1. Niwa H, et al. 2000. Nature Genet. 24:372.
2. Wernig M, et al. 2007. Nature 448:318.
3. Pesce M, et al. 2001. Stem Cells 19:271.

Gene ID
5460 View all products for this Gene ID
UniProt
View information about Oct4 on UniProt.org

Related FAQs

What is the F/P ratio range of our BV421™ format antibody reagents?

It is lot-specific. On average it ranges between 2-4.

Go To Top Version: 2    Revision Date: 08/02/2019

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

BioLegend Inc., 8999 BioLegend Way, San Diego, CA 92121 www.biolegend.com
Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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