- FR70 (See other available formats)
- Regulatory Status
- Other Names
- Ki-24, CD27-ligand, TNFSF7
- Rat IgG2b, κ
- Ave. Rating
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- Product Citations
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CD70 is a type II transmembrane protein. It is a member of the TNF superfamily, expressed on activated B cells, activated dendritic cells, and some activated T cells (low levels). CD70 interacts with CD27 to promote T-B cell cross-stimulation and co-stimulate B cell proliferation and immunoglobulin production. Cells expressing CD70 can co-stimulate T cell proliferation and enhance the production of cytokines. The FR70 antibody blocks CD70 binding to CD27.Product Details
- Antibody Type
- Host Species
- BALB/c mouse B lymphoma A20.2J
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
- The antibody was purified by affinity chromatography, and conjugated with biotin under optimal conditions.
- 0.5 mg/ml
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C. Do not freeze.
FC - Quality tested
- Recommended Usage
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 µg per 106 cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.
- Application Notes
Additional reported applications (for the relevant formats) include: immunoprecipitation1, in vitro blocking of CD70-CD27 binding and inhibition of T and NK cell responses1-3 , and immunohistochemistry4 of aceton-fixed frozen sections. For the most successful immunofluorescent staining results, it may be necessary to maximize signal over background by using a relatively bright fluorochrome-antibody conjugate (Cat. No. 104605/104606) or by using a high sensitivity, three-layer staining technique (e.g., including a biotinylated antibody (Cat. No. 104604) or biotinylated anti-rat IgG (Cat. No. 405402) second step, followed by SAv-PE (Cat. No. 405204)). To reduce non-specific binding to cells bearing Fc-receptors, pre-incubation of cells with anti-mouse CD16/CD32, clone 93 (Cat. No. 101301/101302), is recommended prior to immunofluorescent staining. The LEAF™ purified antibody (Endotoxin <0.1 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. No. 104608).
(PubMed link indicates BioLegend citation)
- Oshima H, et al. 1998. Int. Immunol. 10:517. (Block IP)
- Akiba HH, et al. 1999. J. Immunol. 162:7058. (Block)
- Takeda K, et al. 2000. J. Immunol. 164:1741. (Block)
- Henke A, et al. 2000. J. Virol. 74:4284. (IHC)
- Brown SL, et al. 2007. J. Clin. Invest. 117:258.
- Product Citations
AB_313116 (BioLegend Cat. No. 104603)
- TNF/NGF superfamily, 30-33 kD
Activated B cells and some activated T cells
- T-B cell cross-stimulation
- Cell Type
- B cells, T cells
- Biology Area
- Immunology, Costimulatory Molecules
- Molecular Family
- CD Molecules
- Antigen References
1. Barclay AN, et al. 1997. The Leukocyte Antigen FactsBook Academic Press.
2. Tesselaar K, et al. 1997. J. Immunol. 159:4959.
3. Akiba H, et al. 1999. J. Immunol. 162:7058.
- Gene ID
- 21948 View all products for this Gene ID
- View information about CD70 on UniProt.org
- How many biotin molecules are per antibody structure?
- We don't routinely measure the number of biotins with our antibody products but the number of biotin molecules range from 3-6 molecules per antibody.
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